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  • Study on the Correlation between Body Roundness Index and Metabolically Obese Normal Weight Phenotype in an Elderly Population of Different Genders:Triglyceride Glucose Index as a Potential Influencing Factor

    Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2024-05-15 Cooperative journals: 《中国全科医学》

    Abstract: Background Body roundness index(BRI)is a simple measure of central obesity and is strongly associated with cardiovascular disease. People with metabolically obese normal weight(MONW)have a higher risk of cardiovascular disease and are significantly associated with central obesity. The triglyceride glucose(TyG)index is an indicator of insulin resistance. There are limited studies on the relationship between BRI,TyG index and MONW phenotypes in gender#2;specific elderly populations. Objective To explore differences in the risk of prevalence of the BRI and MONW phenotypes in the elderly population by gender,and to explore the TyG index as a potential factor in their differences by gender. Methods Permanent residents ≥ 60 years of age who underwent physical examination in 10 cities and counties in Anhui Province from 2017-07-01 to 2021-06-30 were selected as study subjects. A unified questionnaire was used to collect information on gender, age,and disease history of the study subjects,collect information on physical examination and laboratory tests,and calculate BRI and TyG indices. Men and women will be classified into metabolically healthy normal weight(MHNW)phenotype and MONW phenotype according to metabolic status and BMI level,respectively:male MHNW phenotype(n=5 384),male MONW phenotype(n=6 251);female MHNW phenotype(n=4 498),female MONW phenotype(n=8 264). Males and females were divided into 4 levels according to BRI quartiles,males:M1,M2,M3,and M4,and females:F1,F2,F3, and F4,respectively.Logistic regression analyses were performed to explore the association between BRI quartile levels and the risk of elevated TyG index and the risk of prevalence of MONW phenotypes respectively,using the Z test was used to compare the differences between the OR value of male and female. Results A total of 24 397 investigators were included in this study, including 11 635(47.7%)males and 12 762(52.3%)females,with a median age of 67(64,70) years,and 9 882(40.5%) with MHNW phenotype and 14 515(59.5%)with MONW phenotype. Hypertension,diabetes mellitus,dyslipidaemia,systolic blood pressure,diastolic blood pressure,TyG index,BRI,fasting glucose(FPG),total cholesterol(TC),triglyceride (TG),and low-density lipoprotein cholesterol(LDL-C)in the elderly population of different genders with MONW phenotype were higher than in the MHNW phenotype,and smoking and high-density lipoprotein cholesterol(HDL-C)were lower than in the MHNW phenotype(P<0.05);prevalence of MONW phenotype,hypertension,diabetes mellitus,dyslipidaemia,TyG index,FPG,and TG were higher than those of low level BRI,and HDL-C was lower than those of low level BRI in the elderly population of different genders with high level BRI(P<0.05). The results of univariate logistic regression analysis showed that the risk of elevated TyG index was increased at BRI M2/F2,M3/F3,and M4/F4 levels in elderly populations of different genders compared with BRI M1/F1 levels(P<0.05);and the risk of elevated TyG index increased with increasing BRI levels(Ptrend<0.001 for male,Ptrend<0.001 for female);and the risk of elevated TyG index was higher at BRI M2,M3,and M4 levels in older men than at BRI F2,F3,and F4 levels in older women(P<0.05). Multifactorial Logistic regression analyses after correction for confounders showed that the risk of MONW phenotype prevalence was increased at the BRI M2/F2,M3/F3,and M4/F4 levels in the elderly population of different genders compared with the BRI M1/F1 level(P<0.05);and the risk of MONW phenotype prevalence increased with the increase of the BRI level(Ptrend<0.001 for male,Ptrend<0.001 for female);and the risk of the MONW phenotype was higher in older men than in older women at BRI levels M2 and M4(P<0.05). Conclusion BRI levels were significantly and positively associated with the risk of the MONW phenotype in older adults of different genders,with high levels of BRI more strongly associated with the risk of the MONW phenotype in older men,with the TyG index being a potential factor contributing to the gender difference.

  • Research Progress of Intelligent Robots in Grassroots Chronic Disease Management

    Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2024-05-15 Cooperative journals: 《中国全科医学》

    Abstract: The increasing prevalence of chronic diseases globally poses major challenges to the health of societies and individuals. Managing chronic diseases requires long-term treatment and monitoring,placing demands on patients' lifestyles. With the aging of the population and changes in lifestyle,chronic disease prevention and control are becoming more and more important. In recent years,as scientific and technological innovation in the field of healthcare develops in depth,and the application of artificial intelligence in healthcare has gradually become one of the important strategic directions of the country,the traditional method of chronic disease management relies too much on the offline communication between the doctor and the patient, which leads to the doctor not being able to maintain long-term and effective communication and follow up with the patient,and the patient may not be able to be detected and monitored by the doctor in a timely manner when his or her condition changes. In addition,the traditional chronic disease management approach is usually a generalized approach that fails to adequately consider the individual differences of each patient. Given the limitations of traditional chronic disease management methods,this study aims to provide more convenient and efficient primary care services using intelligent robots. Through personalized health management plans,assisted medical diagnosis,and timed medication reminders,the intelligent robot is committed to improving patients' quality of life,reducing the pressure on healthcare resources,and promoting the development of intelligent healthcare management globally.

  • The Integrated Development Model and Approach of University Libraries from a Multivariate Collaboration Perspective

    submitted time 2024-05-15 Cooperative journals: 《农业图书情报学报》

    Abstract: [Purpose/Significance] In the current era, integrated and coordinated development is the main trend of social development. University libraries need to explore how the Internet has changed the way people think and promote regional coordinated development to actively explore the integrated development model in a wider range, optimize the efficient allocation of resources in the internal and external integrated development, promote the coordinated development of industries, stimulate innovation capacity, promote the emergence of new models and new formats, and realize the superposition effect of resources and service aggregation and multiplication effects, improve social competitiveness, and promote their sustainable development. [Method/Process] The high-quality development of university libraries requires not only the integration and promotion of certain subject content, but also a comprehensive integration plan and development strategy that takes into account both internal and external factors. University libraries take "cultivating virtue and nurturing people" as the foundation, and improve their resource allocation, management platforms, and service levels through the internal integration construction of development concepts, digital technology, and composite resources. Based on the service of talent cultivation, teaching reform, and discipline construction in universities, university libraries are simultaneously integrating external services with publishers, bookstores, and their cities, seeking a mutually beneficial cooperative development model through the vertical integration of the cultural industry upstream, midstream, and downstream. In the development of multi-collaborative integration, they need to explore the construction of a diversified development system, establish a multi-domain cooperation mechanism, enhance service capabilities, innovate development ideas, and gradually achieve sustainable development goals from consolidating joint forces to stimulating vitality. [Results/Conclusions] Diversified and integrated development is an inevitable trend in the development of the times. In recent years, in the construction and service upgrading of smart libraries, university libraries have achieved certain results in the integration and development of digital academic platform services, new learning ecology construction, virtual real interaction space construction, intelligent service management, and other aspects. However, university libraries have low participation in the integration of extended services with publishers, bookstores, and their cities, and lack a systematic approach in terms of scientific planning and their high-level design has not yet yielded significant social benefits. Overall, there is still a lot of room for innovation and improvement in resource, service and platform integration in university libraries. It is necessary to keep up with the times, seize development opportunities, build an open development system, seek collaborative models of integration and mutual benefits, integrate quality and effectiveness, and achieve innovative transformation and development.

  • Network Analysis of Emergency Information Dissemination Considering the Strength Relationship Between Nodes

    submitted time 2024-05-15 Cooperative journals: 《农业图书情报学报》

    Abstract: [Purpose/Significance] With the rapid development of new media technology, social media platform has become the main carrier of information dissemination. Social network analysis (SNA) is used to study the information dissemination structure and mode of emergencies in microblog, which provides theoretical support for the government to effectively deal with emergencies and crises. [Methods/Process] Taking "Tangshan barbecue restaurant beating incident" as an example, Weibo data were collected to build an information dissemination network with strength relationship between nodes. Social network analysis has been used to analyze the user attributes, node attributes, network attributes and dissemination attributes of the information dissemination network, in order to explore the role of the strength relationship between nodes in emergency information transmission. [Results/Conclusions] 1) The factors of user gender, activity, and region affect their dissemination power. In particular, female users, users with high activity or influence and those in developed provinces have a stronger power of information transmission. 2) The core nodes that plays the role of "bridge" in the dissemination chain is particularly critical. Nodes with strong relationships generally occupy central positions in the information dissemination network and may mainly consist of opinion leaders and mainstream media with greater influence. The pathways of weak and authority relationships are mainly concentrated around a few core nodes, while the pathways of strong relationships are dispersed. 3) Emergency information transmission network has high efficiency and sparse characteristics. 4) The whole process of information transmission is still dominated by weak relationships. Authority relations play an important role in all stages of information transmission, while the role of strong relationships is mainly concentrated in the initial stage. The results of this paper help to deepen the understanding of the patterns and rules of emergency information dissemination, and provide some insights for more effective management and guidance of emergency information dissemination in a particular field such as agriculture. However, our research still has shortcomings, such as insufficient crawling of user attributes and insufficient research methods. In the future study, we will obtain more abundant characteristics of users involved in the dissemination such as age, occupation and education level through interviews or questionnaires, and introduce new methods such as machine learning and graph neural network to predict and analyze the transmission path and node relationship of emergency information, so as to cover these shortcomings, improve the comprehensiveness and effectiveness of the study and deeply explore the information dissemination rules of emergencies.

  • Discussion on Knowledge and Information Transfer Model for University Libraries Serving Rural Revitalization

    submitted time 2024-05-15 Cooperative journals: 《农业图书情报学报》

    Abstract: [Purpose/Significance] The implementation of the rural revitalization strategy is an important decision of the 19th National Congress of the Communist Party of China. It is worth discussing that university libraries should tap the potential of existing resources, better serve the knowledge and information transfer services of rural revitalization, and provide farmers with the necessary knowledge and information transfer services to improve farmers' cultural and information quality, increase agricultural labor productivity, and enrich farmers' spiritual and material lives. [Method/Process] From the perspective of knowledge and information transfer, this paper analyzes the main reasons that affect farmers' knowledge absorption at present, the changing characteristics of knowledge information transfer of university libraries in serving rural revitalization, and the main pathways and shortcomings of universities in serving rural revitalization at present. In order to effectively realize the knowledge information transfer service for most farmers, the study selects university libraries, rural libraries, agriculture-related enterprises and farmers as the subjects and nodes in the knowledge information transfer process and discusses the construction of two knowledge and information transfer pathway models. The first pathway is from libraries to rural libraries (intermediary platforms) to farmers, which mainly transfers general cultural knowledge to improve farmers' cultural quality and information quality. The second pathway is from libraries to agriculture-related enterprises (intermediary platforms) to farmers. This pathway mainly transfers agricultural science and technology, market and other information knowledge to improve farmers' labor productivity. At the same time, the roles and functions of the four participants in the pathway model are analyzed and expounded. In addition, in order to further implement the two new pathways, according to the four processes of knowledge socialization, externalization, integration and internalization described in the SECI theoretical model of knowledge transfer, as well as the transfer contents, transfer methods, transfer objects and the types of knowledge information in the two designed pathway models, the author constructs four knowledge information transfer process models, in which the transfer objects of model 1 and model 2 are rural libraries and farmers, and the transfer objects of model 3 and model 4 are agricultural enterprises and farmers. [Results/Conclusions] Finally, some suggestions are put forward, such as innovating the system and mechanism of university libraries to serve the rural revitalization of knowledge and information transfer, constructing the accurate knowledge information resource base and dissemination system for agriculture, countryside and farmers, improving the ability of rural personnel to obtain knowledge and information, tapping the potential of rural libraries, expanding the channels of knowledge and information transfer, giving play to the role of agriculture-related enterprises, and narrowing the gap of knowledge transfer situation, so as to realize the maximum transfer of knowledge and information to farmers and contribute to the implementation of China's rural revitalization strategy.

  • Transformation and Development of Intangible Cultural Heritage through Technology

    submitted time 2024-05-15 Cooperative journals: 《农业图书情报学报》

    Abstract: [Purpose/Significance] In recent years, the national leadership has attached great importance to the inheritance and innovation of China's excellent traditional culture, and the concepts of creative transformation and innovative development are constantly evolving. The Ministry of Science and Technology of China, among six departments, jointly formulated the "Guidelines for Promoting the Deep Integration of Culture and Technology", proposing to form a "cultural and technological integration innovation system covering key areas and critical links, to achieve the goal of in-depth integration of culture and technology". Intangible cultural heritage (ICH), as an essential part of China's excellent traditional culture, plays a crucial role in consolidating the sense of the Chinese national community and enhancing cultural self-confidence. This paper discusses the research paths for the creative transformation and innovative development of ICH in the context of culture and technology integration, emphasizing the significant role of modern technology in promoting the transformation and development of ICH. [Method/Process] The paper first interprets the connotation of "creative transformation and innovative development" in ICH, and clarifies the theoretical foundation and guiding principles of "creative transformation and innovative development" in ICH. It then analyzes the significant contributions of "creative transformation and innovative development" in ICH to society, economy, and cultural diversity, as well as the categorized scenarios of technology empowerment in "creative transformation and innovative development" of ICH. It discusses six types of common key technologies that enable technology-driven "creative transformation and innovative development" in ICH, including digital technology, virtual and augmented reality, big data analysis, artificial intelligence, cloud computing, and the Internet of Things. It summarizes a three-tier framework system for technology-driven "creative transformation and innovative development" in ICH, namely the method layer, technology layer, and object layer, forming a progressive relationship from theory to technology and then to specific practices. Finally, by integrating the specific example of the traditional craft of Wuhu iron painting, it demonstrates the facilitating role of modern technology in the "creative transformation and innovative development" of ICH and its impact on the protection, inheritance, and innovative development of ICH. [Results/Conclusions] The findings suggest that technological means can effectively protect and inherit ICH, facilitating its creative transformation and innovative development. However, it also requires careful consideration and precautions against the potential risks and challenges that modern technology poses to ICH projects in terms of intellectual property rights, the digital divide, and the indirect nature of experiences. Looking to the future, with the emergence of more innovative technologies, modern technology will not only help to protect and pass on traditional culture but also give new connotations and expressions to traditional culture, ensuring that its unique value and charm continue to play out in an ever-changing world.

  • Evolution of Public Opinion on Public Emergencies and Government Response underthe Influence of Emotional Factors: Based on the Perspective of the Tripartite Game

    submitted time 2024-05-15 Cooperative journals: 《农业图书情报学报》

    Abstract: [Purpose/Significance] With the rapid development of communication technology, the number of Internet users has increased rapidly, and the public opinion on the Internet has also developed according to the requirements of the times. Aiming at the online public opinion of public emergencies under the influence of emotional factors and the government's response strategies, a tripartite game model of netizens, online media and the government is constructed, and its development trend and the influence of emotional factors are simulated and analyzed. [Method/Process] This paper introduces the theory of hierarchical expected utility (RDEU), establishes a tripartite game model of netizens, online media and the government by using the replication dynamic equation, analyzes the influence of emotional factors on the behavioral decision-making of the main body and the development trend of public opinion of the three parties under the influence of different emotional states. Finally, through Matlab software simulation, the influence of emotional state on decision-making is analyzed, and the strategies and suggestions are proposed from the perspective of the government. Hierarchical expected utility theory (RDEU) is introduced to establish a tripartite evolutionary game model, the influence of emotional factors on the agent's behavior decision-making and the development trend of public opinion under the influence of different emotional states are analyzed, and the influence of emotional states on the agent's decision-making is also analyzed. [Results/Conclusions] Under the influence of emotional factors, different combinations of emotional states of each subject produce different results, and all three parties adopt different strategies according to the actual benefits, and the government's own emotions have the most obvious impact on the results of the evolutionary game. When the government has no mood or is in an optimistic mood, the online environment is relatively unstable, and it is necessary to strengthen the management of public opinion; when the government is in a pessimistic mood, the government needs to actively supervise and control public opinion so that public opinion will be well managed, and the network environment will be more harmonious. By analyzing the behavioral strategies of the tripartite game under the influence of emotional factors, the following suggestions are made on the management of public opinion from the government's perspective: 1) The government must always pay attention to the emotions of netizens, try to reduce the participation rate of netizens in online public opinion, strengthen education and improve the quality of netizens. 2) The government should reduce the dissemination of public opinion events by online media, encourage online media to report comprehensively and objectively, and avoid false information that causes negative emotions of the online public opinion.

  • Analysis of Farmers' Information Acquisition Behavior for Digital Inclusi on: Group Focus and Practical Concerns

    submitted time 2024-05-15 Cooperative journals: 《农业图书情报学报》

    Abstract: [Purpose/Significance] In the era of mobile Internet, mobile phones are the most important information access tools. From the perspective of mobile phone use, this paper examines and reveals the information acquisition behavior of farmers, explores the information gap in rural areas, analyzes the characteristics of the information acquisition behavior of key groups, and provide insights into how to improve the information acquisition behavior and narrowing the information gap. The significance of the research is that, with a focus on digital inclusion, we start from the inclusive feature of the Internet and modern information technology, find out the key groups to pay attention to in the information gap, and put forward ideas on how to realize digital inclusion from the perspective of farmers' information acquisition and needs. [Method/Process] Based on the rural sample survey at the national level, principal component analysis and RIF regression analysis were used to measure farmers' information acquisition behavior and identify the role of key influencing factors in improving information acquisition behavior. This study analyzes farmers' information acquisition behavior from the perspective of mobile phone use, describes the micro characteristics of the information gap in rural areas, and makes the conclusion more scientific and generalizable based on the sample survey of farmers at the national level. [Results/Conclusions] The low "long tail" group in the evaluation results of farmers' information acquisition behavior exists in the aged people groups and the groups with the education level below junior middle school, which highlights the phenomenon of information gap in rural areas. Key explanatory variables have a more pronounced marginal effect on groups with low evaluation results of information acquisition behavior (below the median). Improving the impact of mobile phone use among key groups such as the elderly and the undereducated makes an important contribution to narrowing the information gap, which reflects the inevitable requirement of the concept of digital inclusion. Based on the improvement of network facilities, it is also necessary to pay attention to the use of mobile phone functions and information content acquisition of key groups, strengthen the accuracy of information supply, activate the information needs of key groups, and improve the adaptability of such groups in the digital environment. There are still more relatively poor and aging groups in rural areas, which need to be included in the digital inclusion path, improve the situation of vulnerable groups in the digital age, and provide the means to realize people's needs. Future research will pay more attention to promoting the provision of high-quality public service resources through digital tools, realizing the continuous empowerment of digital technology for rural development, and improving the digital literacy of citizens, especially rural residents.

  • Developing the New Quality Productivity : Responses and Reflections on the Discipline of Information Resource Management

    submitted time 2024-05-15 Cooperative journals: 《农业图书情报学报》

    Abstract: The scientific connotation, strategic considerations and practical ways of "new quality productive forces" have received wide attention from the political, academic and industrial circles. Cultivating and developing new quality productive forces means fundamentally changing the mode of production. It is necessary to grasp the practical direction of new quality productive forces from their scientific connotation and internal logic, and explore the scientific way of cultivating and developing the new quality productive forces from China's national conditions. As an important engine of economic development in the new era, its connotation characteristics, development path, opportunities and challenges are worthy of further discussion. For this reason, seven experts were invited to share their perspectives. 1) The deep logic and realization path of the construction of new quality productive forces and high-quality data resources. This paper discusses in depth the background and connotation characteristics of the high-quality data resources, and analyzes the internal logic of the mutual promotion between high-quality data resources and the development of new quality productivity. It is proposed that the construction of high-quality data resources must implement the concepts of innovation, coordination, openness, credibility and sustainability, and follow the construction strategies of concept innovation, model innovation, structural innovation and technological innovation. 2) The theoretical logic and practical path of data elements empowering new quality productive forces. In the new era of green and intelligent development, there is a profound dialectical relationship between data elements and new quality productive forces, and a new situation of economic and social development is accelerated in the spiral ascent of complementarity. We should comprehensively build a data space governance model with clear data ownership, smooth data path, guaranteed data quality and orderly and standardized algorithm computing power, expand new quality production capacity and production factors with the goal of human-machine cooperation and promote sustainable economic and social development. 3) Accelerating the promotion of data rights. Data can act on new quality productive forces from the technical, factor and industrial dimensions, and accelerate the formation of new quality productive forces. The multiplier effect of data elements on new quality productive forces will involve many dimensions, levels and stages, the most important and fundamental of which is how to determine the ownership of data. The measures for speeding up the promotion of new quality productive forces are discussed, such as constructing the theory of property rights in accordance with the characteristics of data and the law of development of new quality productive forces, speeding up the clarification of the types and scope of data confirmation, and improving the supporting system of data confirmation. 4) Promoting China's modernization path with Chinese-style digitization. Accelerating the promotion of Chinese-style digitization is not only the only way to realize Chinese-style modernization, but also the core to fully release new quality productive forces and build China's asymmetric competitive advantage under the new situation. It is suggested that in the process of digitalization and informatization in China, the information resource management industry plays a vital role and bears the important responsibility of digital transformation. 5) Interpreting the mission of public libraries from the perspective of new quality productive forces. Since its establishment, public libraries have been based on the concept of enlightenment. Faced with the profound digital transformation of society, today's public libraries need to further develop the potential of empowering the people in the field of digital economy and make them play an active role in the development of new quality productive forces. Public libraries need to further develop their potential to empower people in the field of digital economy, so that they can play an active role in the development of new quality productive forces. They should actively participate in data commercialization, promote the effective use of data elements and the efficiency of resource allocation, and enable more people to obtain and use data elements equally through open access and digital services. 6) The intelligent transformation of university libraries from the perspective of new quality productive forces. In the process of developing new quality productive forces, we should fully understand the profound meaning of new productive forces, based on the core tasks of providing a strong guarantee for the development of new quality productive forces in higher education, grasp the development opportunities, and realize the high-quality development of the industry. This paper explains the understanding of university libraries on the three key words of new quality productive forces, and proposes that the re-understanding and realization of library intelligence is an important starting point for the development of new productive forces. 7) To support information resource management discipline to accelerate the development of new quality productive forces. From the perspective of information resource management discipline, this paper analyzes several aspects of "doing something" under the rise of new quality productivity concept, including theoretical system, information resource guarantee, information analysis and consultation, scenario building, knowledge popularization and civic literacy cultivation. The content of academic evaluation, information policy, intellectual property rights, digital consumption behavior and other directions related to new quality productivity are also important directions that the discipline needs to pay attention to.

  • Microhabitats and their colonized plants on rock outcrops surface in tropical karst forest

    Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》

    Abstract: Rock outcrop is a typical feature of karst ecosystem, on which there are complex microhabitats and various colonized plants, but the relationship between these plants and microhabitats is not clear. Taking a tropical seasonal humid forest in Xishuangbanna as the research object in this study, we measured the morphological characteristics and external environmental factors of 586 microhabitats on rock outcrops surface. All colonized vascular species on these microhabitats were identified and counted. Then, Pearson correlation analysis and redundancy analysis (RDA) were used to reveal the relationship between microhabitats (including morphological characteristics and environmental factors) and vascular plants richness. The results were as follows: (1) In tropical karst forest, the microhabitat characteristic such as soil depth [(4.92±4.00)] cm and area [(532.28±1 575.10) cm2] have great variability. (2) A total of 1 518 individuals of 90 species belonging to 82 genera and 44 families were recorded in all microhabitats, and karst preferent species that dominants on limestone accounted for 35.6%. (3) The area size and soil depth of microhabitat can explain more than 70% of species composition and colonized plants richness in RDA model. (4) The species-area relationship between the richness of the colonized plants and the area of rock outcrops microhabitats shows an obvious exponential decay. In conclusion, microhabitats on rock outcrops in the tropical karst forest is highly heterogeneous, the area size and soil depth are the key factors affecting plant colonization, and the species-area relationship dominates the species richness pattern on rock outcrops microhabitats.

  • Cloning, structural and function analysis of MiMYB44L gene in kernels of Macadamia integrifolia

    Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》

    Abstract: Macadamia nut (Macadamia integrifolia) is an evergreen nut tree with high economic value. Its kernel is rich in nutrients such as fatty acid and protein, etc. In order to further explore the main regulatory genes related to nutrient formation in M. integrifolia kernels, transcriptomics, gene cloning, fluorescence quantification PCR and bioinformatics techniques were used to screen potential regulatory genes from the kernel transcriptomes of ‘Guire No. 1’ and ‘A4’, which have significantly different nutrient content in M. integrifolia kernels. The results were as follows: (1) Transcriptome analysis showed that 1 667 genes were up-regulated and 1 798 genes down-regulated in ‘Guire No. 1’ kernel compared with those of ‘A4’ kernel and KEGG enrichment analysis showed that the differential genes were mainly in starch and glucose metabolism, amino acid biosynthesis and carbon metabolism. (2) A significant differential gene-LOC122077931 encoding the R2R3-MYB transcription factor MYB44L was discovered. The MiMYB44L gene was cloned in kernels of M. integrifolia variety ‘Guire No. 1’ using RACE technology, which was 1 165 bp in length, 999 bp in ORF in length, and encoded 332 amino acids. (3) Bioinformatics analysis confirmed the presence of the SANT domain in the MiMYB44L protein, a hallmark feature of the R2R3-MYB family. The protein lacked both a signal peptide and a transmembrane domain but featured phosphorylation sites. (4) The protein content in kernels of 10 M. integrifolia varieties was determined. And it was found that the expression of MiMYB44L gene in M. integrifolia varieties with high protein content was significantly higher than that in varieties with low protein content, and the overall correlation coefficient was 0.54, reaching a very significant level. The results of this study provide theoretical guidance for in-depth analysis of the regulatory mechanism of MiMYB44L gene in the formation of protein content in M. integrifolia.

  • Composition and floristic characteristics of national key protected wild plants distributed in Hainan Province, China

    Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》

    Abstract: Based on the ‘National Key Protected Wild Plants List’ and related data in 2021, combined with field investigation, the ‘National Key Protected Wild Plants List of Hainan Province’ was integrated, and its composition and floristic characteristics were analyzed. The results were as follows: (1) Among the national key protected wild plants, the original Cycas changjiangensis, C. hainanensis, C. lingshuigensis and C. taiwaniana were incorporated into C. taiwaniana; compared with the National Key Protected Wild Plant List in 1999, the original Semiliquidambar cathayensis and Amphicarpaea linearis were removed from the protection category, and Bretschneidera sinensis was degraded from the Category I protection to the Category II protection.127 species of national key protected wild plants were added, and a total of 173 species ( including varieties ) were added, belonging to 53 families and 83 genera, including 8 species of national Category I protected wild plants. There are 165 species of national Category II protected wild plants and 32 endemic species in Hainan Province, which belong to 4 groups. Among them, the angiosperm species are the most abundant, with 129 species. (2) Based on years of field investigation information and the results of this survey, Cycas rumphii, C. shanyagensis, Alsophila costularis and Paphiopedilum purpuratum have not been investigated. (3) The life forms of 173 protected plants were divided into 5 types, mainly phanerophytes ( 115 species ), followed by hemicryptophytes ( 34 species ) and hemicryptophytes ( 22 species ), and hemicryptophytes and cushion plants were the least ( 1 species ). (4) At the family level, Orchidaceae had the most species, including 47 species ( 27.17% ). At the genus level, Dendrobium has the largest number of species, including 21 species ( 12.14% ). (5) The floristic elements are complex and have obvious tropical characteristics. The families are mainly distributed in the pantropics and their variants. The genera are mainly distributed in tropical Asia and its variants. In summary, the tropical nature and unique phenomena of national key protected wild plants in Hainan Province are obvious, and there are many new protected species. Therefore, background investigation and dynamic monitoring should be carried out continuously, law enforcement should be strengthened, human factors should be reduced to interfere with and destroy habitats, and in-situ and ex-situ protection measures should be taken to strengthen protection and management.

  • Effects of water and soil microorganisms on the growth traits of Albizia kalkora and Leucaena leucocephala in a dry-hot valley

    Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》

    Abstract: Vegetation restoration is an effective way for ecological restoration in the dry-hot valley, and many alien species are introduced along with natural vegetation restoration. In order to compare the responses of native and alien species to water and microorganisms, the native Albizia kalkora and alien Leucaena leucocephala were selected as our research objects, and the effects of water and soil microorganisms on the growth and development of the two plants were studied by soil moisture control and soil microbial inoculation under climate chamber conditions. The results were as follows: (1) Compared with Leucaena leucocephala, the seed germination rate, root biomass, root-shoot ratio and plant nitrogen-phosphorus ratio of Albizia kalkora were significantly increased by 58.82%, 76.13%, 90.59% and 18.42%, respectively (P<0.05); (2) Under the drought conditions, the root biomass and plant nitrogen concentration of Albizia kalkora were significantly lower than those under moist conditions by 33.67% and 8.65% (P<0.05), and the plant height, aboveground biomass and root biomass of Leucaena leucocephala under drought conditions were 39.32%, 40.06% and 39.61% lower than those under moist conditions (P<0.05), indicating that drought inhibited the growth and nutrient uptake of both plants; (3) The number of nodules in the roots of Albizia kalkora under drought conditions was higher than that under moist conditions (P<0.05), while the number of nodules in the roots of Leucaena leucocephala under moist conditions was higher than that under drought conditions; (4) Except for the proportion of dead leaf biomass, soil microbial inoculation and the interaction between soil microorganisms and water had no significant effect on the growth of Albizia kalkora and Leucaena leucocephala. The above results suggest that Albizia kalkora is more suitable for drought environment than Leucaena leucocephala, which provides a theoretical basis for the selection of species for vegetation restoration in dry-hot valleys, but the effects of soil microorganisms on plant growth are worthy of further study.

  • Geographic distribution and research progresses of National Key Protected Wild Plants in Hubei Province

    Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》

    Abstract: The updated List of National Key Protected Wild Plants (LNKPWP) was released by 1National Forestry and Grassland Administration and Ministry of Agriculture and Rural Affairs of China in September 2021. By integrating literature search, herbarium record, field investigation and expert consultation, we compiled the updated list, investigated the geographic map, research progresses, threatened status, and protection status of National Key Protected Wild Plants (NKPWP) in Hubei Province. The results were as follows: (1) There are 155 species on the LNKPWP in Hubei Province, of which 11 species belong to Category I and the remaining 144 species belong to Category II. (2) Geographic distribution of NKPWP in Hubei Province is uneven and concentrated in west and southwest areas, with Shennongjia and Lichuan have the greatest numbers of NKPWP. (3) The most studied species are the species with economic value, flagship species and regional representative species. In addition, more than a third of the species (55 species) were almost unstudied. The research of NKPWP in Hubei Province focused on genetic diversity, genetic structure, future distribution prediction under global change conditions, and phylogeography. (4) According to the “China Red List of Biodiversity-Higher Plants Volume”, the NKPWP in Hubei Province contain 9 critically endangered species, 30 endangered species, 41 vulnerable species and 19 near-threatened species. The main threatened factors are direct mining or logging, habitat degradation or loss, and species intrinsic factors. (5) Until now, 137 species(88%) of NKPWP in Hubei Province are wholly or partially located in protected areas, and the remaining 18 species (12%) are completely located outside protected areas. At present, 93 species of NKPWP have been introduced and cultivated in Hubei Province, while the other 62 species have not been recorded under ex situ conservation. In addition, very few species have been reintroduced into the wild. (6) At present, although the distribution ranges of seven wild plants in Hubei Province (five of which are endemic to Hubei) are limited and they are negatively affected by internal causes and human interference, they have not been included in the LNKPWP. In addition, this study points out the knowledge gaps in the research and protection of NKPWP in Hubei Province, and puts forward specific research suggestions and protection strategies.

  • Effects of nitrogen forms on nitrogen uptake and allocation and andrographolide components accumulation in Andrographis paniculata

    Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》

    Abstract: Nitrogen (N) is a mineral nutrient with the highest demand for plants, and it is also a vital abiotic factor that limits plant yield and quality. Plants use inorganic and organic N sources from soil, and N form is a key factor affecting the active ingredients and their contents in medicinal plants. However, the utilization characteristics of different N forms by medicinal plants and the impact on active ingredients remain to be investigated. With nitrate nitrogen (NN), ammonium nitrogen (AN), amide nitrogen (urea, UN) and amino acid nitrogen (glycine, GN) as sole N source and combining 15N isotope tracing and physiological and biochemical analysis, this study aimed to clarify the characteristics of N uptake and utilization and its effect on the accumulation of andrographolide components at different growth stages (rapid growth stage, jointing stage, budding stage, flowering stage) of Andrographis paniculata. The results were as follows: (1) N content in leaves and roots decreased gradually with the growth period, and it was lower in the NN treatment. (2) The absorption rate of N was higher in the vegetative growth period, and rapidly decreased in the reproductive growth period. The absorption rate of AN, UN and GN was higher than that of NN in A. paniculata. (3) The allocation ratio of N in leaves was decreased, while the allocation ratio of N in stems was increased at budding stage. In comparison to the NN treatment, AN, UN, and GN treatments reduced N allocation ratio in leaves, but increased the ratio in stems and roots at this growth stage. (4) At rapid growth stage, the maximum carboxylation rate and maximum electron transport rate of photosynthesis were lower in the NN treatment, as well as the proportion of leaf N allocation in the carboxylation system and bioenergetics components. However, the allocation of leaf N in the carboxylation system in UN and AN treatment was reduced at budding stage and flowering stage, respectively. (5) AN, UN and GN increased andrographolide and dehydroandrographolide contents, and decreased 14- deoxyandrographolide content at budding and flowering stages, while neoandrographolide was slightly affected by N forms. (6) The contents of andrographolide and neoandrographolide were significantly negatively correlated with N contents in leaves, stems and roots, N uptake rate, and N allocation ratio in leaves and roots, while they were significantly positively correlated with N allocation ratio in stems. The opposite was true for 14-deoxyandrographolide. Taken together, the results indicate that the vegetative growth period is the main period for N uptake in A. paniculata, and that A. paniculata can better utilize AN, UN, and GN, and promote the accumulation of andrographolide components by optimizing N allocation. The results provide a theoretical reference for N management of A. paniculata.

  • Isolation and identification of symbiotic fungi in roots of young Quercus wutaishansea on Liupan Mountain

    Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》

    Abstract: The symbiotic fungi in roots of young Quercus wutaishansea at different ages were isolated directly from root tips to elucidate community structure and dynamic changes. The isolated fungi were identified based on the morphological characteristics of colony and molecular analysis. The results were as follows: (1)A total of 249 fungal strains were isolated from the roots of young Q. wutaishansea, 18 species belonging to 15 genera, 8 families, 7 orders, 8 classes and 2 phyla were recognized. Among them, 2 species are ascomycotes and 16 species are basidiomycets. Pezicula pruinosa was the species with the highest isolation frequency (81.93%), followed by Dactylonectria torresensis (4.02%), Ilyonectria robusta (2.01%) and Atrocalyx nordicus (2.01%). (2)Significant differences in species and quantities of symbiotic fungi demonstrate in different ages of young Quercus wutaishansea, with the highest frequency of isolation occurring in the 4-5 years old (44.98%) followed by the triennial (29.32%), biennial (19.68%) and annual (6.02%). To sum up, culturable symbiotic fungi in the roots of young Q. wutaishansea on Liupan Mountain were highly diverse. Species and quantities of isolates varied at different ages of trees, and the diversity increased with the growth of tree. Pezicula pruinosa is the most dominant culturable symbiotic fungi in roots of young Quercus wutaishansea. The results lay the foundation for further investigating symbiotic fungal resources and exploring the microbial symbiotic mechanism of Q. wutaishansea in adapting to local environment.

  • Cloning and expression of aerenchyma-related gene SmPAD4 in Saussurea medusa

    Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》

    Abstract: The aerenchyma is an adaptive structure of Saussurea medusa in response to extreme environments, and its formation is usually accompanied by programmed cell death (PCD). The death of cells and the formation of aerenchyma are typically regulated by the PAD4 gene (Phytoalexin deficient 4). However, the mechanism by which SmPAD4 regulates the formation of aerenchyma in S. medusa remains unclear. In this study, S. medusa was used as the experimental material, and the gene SmPAD4 related to ventilation tissue formation was cloned by homologous cloning and RACE technology, and its sequence, phylogenetic evolution, expression and subcellular localization were analyzed, and its promoter was amplified by hi-1IL PCR technology to explore its function in environmental adaptation. The results were as follows:(1) The cDNA of SmPAD4 gene was successfully cloned with a total length of 2 047 ( GenBank accession number OR766038), including an open reading frame of 1 866 bp, encoding 621 amino acids, a molecular formula of C3163H4906N848O910S26. The protein was an alkaline and hydrophilic unstable protein. (2)Phylogenetic tree analysis showed that SmPAD4 had high similarity with CcPAD4 of Cynara cardunculus. (3)A 1 049 bp promoter sequence of SmPAD4 was amplified, which included cis-acting elements such as light response element, hypoxia response element, methyl jasmonate response element, dry early, auxin response element and binding site of WRKY transcription factor.(4)Real-time quantitative fluorescence analysis showed that SmPAD4 gene was expressed in roots, stems and leaves, and the expression level was the highest in leaves. Under ultraviolet and hypoxia stress, the expression of SmPAD4 gene was up-regulated in leaves and stems, and down-regulated in roots. ( 5 ) Subcellular localization showed that SmPAD4 is distributed in the nucleus, cell membrane, and chloroplast. The results show that SmPAD4 gene has a unique protein domain and it responds to hypoxia and ultraviolet environmental stress, so it plays an important role in the formation of aerenchyma and the response to adversity stress. This study provides theoretical basis for further exploring the role of SmPAD4 gene in the environmental adaptation process of S. medusa.

  • Ethnobotanical survey and research on bsang plants in Xizang Autonomous Region

    Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》

    Abstract: Bsang is a folk activity with unique ethnic cultural characteristics on the Qinghai-Tibet Plateau (QTP), and it plays an important role in the daily life of Zang people. There are abundant bsang plant resources and related bsang cultural knowledge on the QTP, to investigate, document, and research Xizang bsang plants and related traditional knowledge, the research team conducted four ethnobotanical surveys on bsang plants in Xizang. Interviews were conducted with 459 informants from 22 townships, documenting 83 species of bsang plants belonging to 36 genera across 19 families. The results were as follows: (1) People of different regions, ages, and genders possess extensive knowledge of bsang plants; (2) Hordeum vulgare var. coeleste, Juniperus indica, Rhododendron anthopogon and have high cultural value (CV) indices, making them crucial in bsang activities; (3) The utilization of bsang plants demonstrates regional and substitutive characteristics, reflecting the local practices of scientifically managing and sustainably collecting of bsang plant resources. This study contributes to the inheritance of bsang activities and the biodiversity conservation and sustainable utilization of bsang plant resources.

  • Effects of continuous cropping on fungal community structure succession in rhizosphere and non-rhizosphere soils of cassava

    Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》

    Abstract: For revealing the mechanism of cassava continuous cropping obstacle formation, the current study planted cassava in a fixed field for three consecutive years, and applied high-throughput sequencing technology and bioinformatics to explore effects of continuous cropping on fungal community structure of both rhizosphere and non-rhizosphere of cassava soils. The results were as follows: (1) Continuous cropping caused significant effects on the α diversity and β diversity of rhizosphere and non-rhizosphere soils of cassava. (2) The dominant fungi phyla of cassava rhizosphere and non-rhizosphere soils were Ascomycota, SAR_k__norank, Basidiomycota, Mucoromycota, and unclassified _k __Fungi, while the main classes were Sordariomycetes, Eurotiomycetes, Dothideomycetes and Intramacronucleata. The composition of Ascomycota of the non-rhizosphere soils varied with the continuous cropping years significantly. In the rhizosphere, Hypocreales, Chaetothyriales, Myrothecium, Dothideomycetes, and Sordariomycetes were observed in 2019 and 2020, while Monosiga was found in 2021. (3) Soil pH, organic matter content, available nitrogen content, available phosphorus content, available potassium content, and urease activity significantly influenced the structure of fungal communities, especially for distributions of Ascomycota, SAR_k__norank, Basidiomycota, and Mucoromycota. In conclusion, continuous cropping of cassava influenced soil physicochemical properties through the accumulation of root exudates, thus altering the survival environment of fungi, leading to differences of the diversity and the abundance of fungal communities between cassava rhizosphere and non-rhizosphere soils. The relative abundance of Hypocreales, Myrothecium, and Sordariomycetes decreased with continuous cropping years, which may be one of main causes of the continuous cropping obstacles of cassava.

  • Floristic geography of woody plants in the big plot of Gulinqing karst forest in Yunnan Province

    Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》

    Abstract: Based on the 25 ha karst forest big plot in Gulinqing, Yunnan, through sample-plot investigation, specimen collection and identification, floristic analysis, Jaccard similarity coefficient and PCA, we explored its floristic characteristics and the status of the Gulinqing big plot in Yunnnan and the geographical relationships among Gulinqing in Yunnan, Bubeng in Yunnan, and Nonggang in Guangxi, China. The results were as follows: (1) There were about 78 families, 238 genera, and 406 species(including infraspecies taxa and excluding woody vines)of woody plants with DBH≥ 1cm. (2) In the plot, the families with two–five species and with one species were dominant and accounted for 37.18% and 34.62% of the total number of families, respectively, while the genera with two–four species and with one species were also dominant and accounted for 30.25% and 65.13% of the total number of genera, respectively. (3) Through analysis of the areal-types of families and genera, it was found that it had been divided into 9 areal-types and 7 variations at the family level, and the proportion of families was the highest at Pantropic, accounting for 50.77%, and the ratio of tropical flora to temperate flora (R/T) was 4.42; there were 11 areal-types at the genera level, mainly composed of Tropical Asian, Pantropic, Old World Tropics, 33.47%, 18.22%, and 13.98% respectively, and with R/T of the genera was 10.25. (4) The composition of Gulinqing, Bubeng and Nonggang big plots showed that Gulinqing and Bubeng had the highest similarity in family and genera level at Jaccard similarity coefficient (0.674 and 0.395, respectively), and Bubeng and Nonggang with the smallest Jaccard similarity coefficient (0.575 and 0.297, respectively). (5) PCA principal component analysis of the floristic spectrum had similar result of the Jaccard similarity coefficient above in the level of genera, but different one at the level of families, e.g. a higher geographical connection between Gulinqing and Nonggang. In summary, this plot has a high diversity of families and genera, significant tropical characteristics, ancient floristic characteristics, obvious transitional status and extensive flora connections. However, there is an inconsistent geographical connection among Gulinqing, Bubeng and Nonggang from the composition and floristic spectrum.