Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-01-25 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the effect of low-selenium diet on the liver and kidneys of rats and explore the role of macrophage polarization into M1 and M2 phenotypes in liver and kidney injuries. Methods Twenty-four rats (12 female and 12 male) were randomly divided into control group and low-selenium group and fed with normal chow (dietary selenium of 0.18 mg/kg) and low-selenium diet (dietary selenium of 0.02 mg/kg) for 109 days.After the feeding, the rats were sacrificed for HE staining to observe liver and kidney pathologies, and immunohistochemistry was performed for analyzing CCR7, CD206, CD163-positive cell numbers in the liver and kidneys. Results The rats in low-selenium group showed severer fibrosis in the liver and kidney than the control group. In either male or female rats in low-selenium group, CCR7 and CD206 expressions in the liver were comparable with those in control group, but CD163 expression was lower than that in the control group (P<0.05 for both female and male rats). In the kidney, the proximal tubule showed a slightly higher while the distal tubule showed a slightly lower CCR7 expression in low selenium group than in the control group (P>0.05). In low-selenium group, a significantly lower CD163 expression in the distal tubule and a significantly higher CD206 expression in the proximal tubule were noted as compared with the control group (P<0.05 in both female and male rats). Compared with the control rats, the male rats in low-selenium group, but not the female rats, showed a significantly lower CD163 expression in the proximal tubule of the kidney (P<0.05); the female but not the male rats in low-selenium group show a higher CD206 expression in the distal tubule (P<0.05). Conclusion Low-selenium diet can cause liver and kidney fibrosis in rats and may inhibit macrophage activationintotheM2phenotype.
Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2023-07-04 Cooperative journals: 《中国全科医学》
Abstract： Background With the accumulation of high-quality evidence，the clinical effectiveness of acupuncture for migraine has been increasingly recognized. Numerous acupuncture clinical studies in China highlight and validate various acupuncture intervention schemes through clinical trials. Integrating and selecting these advantageous schemes to guide clinical practice has become a new focus in acupuncture research. Objective To conduct multicriteria decision making（MCDM）by using technique for order preference by similarity to an ideal solution（TOPSIS）method based on meta-analysis，screen OPT of acupuncture for migraine and explore the ideas for constructing OPTs of acupuncture. Methods Wanfang Data Knowledge Service Platform，VIP，CNKI，SinoMed，PubMed，Web of Science，and Embase were searched by computer for randomizedcontrolled trials（RCTs）on acupuncture treatment for migraine published before April 2022. Two researchers independently screened the literature，extracted basic characteristics of literature〔author，year of publication，sample size，cure rate，efficiency rate and visual analogue score（VAS），etc.〕，and assessed the quality of the RCTs by using modified Jadad scale. Acupuncture treatment plans for migraine were summarized based on the characteristics of the included RCTs，andmeta#2;analysis was performed. The relative risk（RR）of cure rate and efficiency rate，and the mean difference（MD）of VAS scores for each plan were extracted based on the meta-analysis results. The treatment frequency and score of the modified Jadad scale were calculated for each plan using sample size as the weight. The data were all included in the MCDM analysis，the weights of different decision criterion were determined using the entropy weight method. The TOPSIS method was finally used to select OPT of acupuncture for migraine. Results A total of 17 RCTs were included and categorized into 8 types of treatment plans of acupuncture for migraine（Tongyuan acupuncture，six-spirit points，bone-nearby acupuncture，conventional acupuncture，Gallbladder Meridian acupoints，head acupoints，penetration needling method and Siguan acupoints）according to the results of meta-analysis. The TOPSIS evaluation indicated that the treatment plan of acupuncture focusing primarily on head acupoints is OPT，involving needling the head acupoints of Fengfu，Tiantu，Head Yingxiang，Taiyang，Shuaigu，and Fengchi once daily or every other day，with an average treatment number of 11，with the main advantages of shorter treatment time and significant reduction in VAS pain scores. Conclusion The OPT of acupuncture for migraine screened and constructed in the study can provide reference for clinical application. The utilization of the entropy weight-TOPSIS method based on meta-analysis presents a novel approach for constructing the OPTs of acupuncture for migraine.
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-06-15 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the protective effects of paeoniflorin against PM2.5-induced damage in BEAS-2B cells and explore the possible mechanism. Methods With a factorial design, this study was performed to observe the protective effects of different doses of paeoniflorin against PM2.5-induced BEAS-2B cell growth inhibition and the effects of paeoniflorin on the contents of malondialdehyde (MDA) and intracellular reactive oxygen species (ROS) in the cell cultures. Results Exposure to increased PM2.5 concentrations caused significant decrease in the cell survival rate (P<0.05) with a clear dose-response relationship (r=-0.759, P<0.05). Treatment of the cells with paeoniflorin significantly attenuated PM2.5-induced inhibition of BEAS-2B cell survival (P<0.05), but the effect of paeoniflorin was not dose-dependent (P>0.05). PM2.5 exposure also significantly increased the contents of MDA and intracellular ROS (P<0.05), and paeoniflorin obviously antagonized these effects of PM2.5. Conclusion Paeoniflorin can protect BEAS-2B cells from PM2.5-induced growth inhibition, and the mechanism might be related to the anti-oxidant effects of paeoniflorin.
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