分类： 生物学 >> 生物物理学 分类： 材料科学 >> 生物材料 分类： 化学 >> 化学生物学 分类： 医学、药学 >> 基础医学 提交时间： 2023-02-09
摘要： Nanotechnology has provided considerable promise for the biological and medical fields, especially in the subjects of biological and medical imaging for the last two decades. Here, we outline different nanoparticles to contribute to biological and medical imaging disciplines. These concerned nanoparticles are soft nanoparticles, which are based on biomacromolecule/polymer or organic molecule components, hard nanoparticles that are derived from various inorganic components and hard-soft nanoparticles that are based on both inorganic components and biomacromolecule/polymer or organic molecule ones. We also discuss the imaging modalities in biology and medicine that various nanoparticles became involved in are: (1) optical imaging (OI), (2) computed tomography (CT), (3) magnetic resonance imaging (MRI), (4) ultrasonography (USG), (5) positron emission tomography (PET). We will also describe various nanoparticles to serve for one/some of those five modalities in biology and medicine imaging in this review paper.
分类： 材料科学 >> 纳米科学和纳米技术 提交时间： 2022-10-04
摘要： Nanotechnology has provided considerable promise for the biological and medical fields, especially in the subjects of biological and medical imaging for the last two decades. Here, we outline different nanoparticles to contribute to biological and medical imaging disciplines. These concerned nanoparticles are soft nanoparticles, which are based on biomacromolecule/polymer or organic molecule components, hard nanoparticles that are derived from various inorganic components and hard-soft nanoparticles that are based on both inorganic components and biomacromolecule/polymer or organic molecule ones. We also discuss the imaging modalities in biology and medicine that various nanoparticles became involved in are: (1) optical imaging (OI), (2) computed tomography (CT), (3) magnetic resonance imaging (MRI), (4) ultrasonography (USG), (5) positron emission tomography (PET). We will also describe various nanoparticles to serve for one/some of those five modalities in biology and medicine imaging in this review paper.
分类： 医学、药学 >> 基础医学 提交时间： 2018-06-15 合作期刊: 《南方医科大学学报》
摘要： 目的:探讨色素上皮衍生因子（PEDF）是否通过作用上皮间质转化（EMT）抑制乳腺癌浸润转移。方法免疫组化方法检测119例浸润性导管癌组织中PEDF、vimentin、E-cadherin表达情况；构建PEDF-siRNA-vector干扰载体，应用RNA干扰技术阻断乳腺癌SK-BR-3细胞中PEDF表达，并构建重组腺病毒载体构建（Lentivirus-PEDF-vector），转染SK-BR-3细胞。采用细胞划痕实验、细胞侵袭实验、Western bolt方法检测SK-BR-3细胞中PEDF表达变化，并釆用Western bolt方法检测乳腺癌细胞上皮性标志物E-cadherin和间质性标志物vimentin的表达改变，并观察乳腺癌细胞的体外增殖、侵袭、粘附特性的改变。统计分析采用卡方检验、Fisher精确概率法检验Spearman等级相关检验、配对计数资料检验。结果乳腺浸润性导管癌中PEDF阳性表达率明显低于正常乳腺组织，PEDF阳性表达与肿瘤大小相关，与E-cadherin表达正相关（r=0.473，P<0.001）, 与vimentin表达呈负相关（r=-0.412，P<0.001）。乳腺癌SK-BR-3细胞在PEDF条件培养基培养后：细胞形态学发生改变；Transwell侵袭实验表明：细胞侵袭转移力减弱；PEDF-siRNA增加SK-BR-3细胞的迁移与侵袭，而Lentivirus-PEDF-vector抑制SK-BR-3细胞的侵袭与迁移能力。Western blot结果显示：细胞中E-cadherin表达明显减少（P<0.05），vimentin表达明显增多（P<0.05）。结论PEDF基因与乳腺癌的浸润转移过程密切相关，PEDF可作用SK-BR-3细胞发生EMT进而抑制乳腺癌的浸润转移。
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