Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2023-09-21 Cooperative journals: 《中国全科医学》
Abstract： Background In recent years，the symptoms of perimenopausal hearing loss（PMS-HL）have received widespread attention，but there is no targeted treatment. Zuoci pill has been used clinically for a hundred years，but there is no relevant research on the treatment of PMS-HL with this drug. Objective To explore the mechanism and therapeutic targets of Zuoci pill on PMS-HL through further validation of animal experiments based on the network pharmacology and molecular docking technology. Methods The active components and action targets of Zuoci pill were obtained through TCMSP and Uniprot databases，and the protein targets of PMS-HL were obtained through GeneCards，OMIM，TTD，DrugBank，and PharmGKB databases according to the composition of Zuoci pill from inception to February 2023，the intersection was taken to screen the potential therapeutic targets of Zuoci pill for the treatment of PMS-HL，and the "drug-active ingredient-target" interaction network diagram was constructed. The protein interaction analysis function of the String database was utilized to screen core targets. The Metascape database was used for enrichment analysis of the function of the proteins and pathways. Molecular docking of the core proteins was performed using Autodock and Pymol software to determine the binding capacity between key active ingredients and core targets. The menopausal animal model was established from September 2022 to January 2023，including Sham operation group（SHAM）group，ovariectomized（OVX）group，and Zuoci pill（ZCW）group，and serum level of the key protein interleukin 1β（IL-1β）was detected and statistically analyzed using SPSS 26. Results There were 90 active components，226 potential protein targets，2 481 PMS-HL related targets，and 150 Zuoci pill and PMS-HL intersection targets for the compounds in the Zuoci pill formula. A total of 183 items for biological process，103 items for molecular function，and 103 items for cellular components were obtained in the Gene Ontology（GO）function. The top three Kyoto Encyclopedia of Genes and Genomes（KEGG）were cancer pathways，lipid and atherosclerotic lesion pathway，and receptor activation pathway for chemical carcinogenesis. Molecular docking showed that the main active ingredients of Zuoci pill in treating PMS-HL were quercetin，kaempferol，stigmasterol，β- Sitosterol，isorhamnetin，diosgenin，tetrahydrostilbene and kaempferone；the active components of Zuoci pill can bind to the core target proteins of serine/threonine kinase 1（AKT1），cellular tumor antigen p53（TP53），interleukin 6（IL-6），vascular endothelial growth factor（VEGFA），cystatinase 3（CASP3），IL-1β， epidermal growth factor receptor（EGFR），and estrogen receptor 1（ESR1）stably. Comparison of serum IL-1β level among the three groups of rats showed statistically significant difference（F=11.73，P<0.001）. Conclusion The 90 active ingredients in Zuoci pill，such as quercetin，act on 226 potential proteins such as AKT1 to regulate the antioxidant stress of tissues and cells，metabolism of blood lipids and glucose，and anti-tumor pathway，directly or indirectly protect the perimenopausal hearing function，IL-1β may be one of the key target proteins.
Subjects: Medicine, Pharmacy >> Preventive Medicine and Hygienics submitted time 2023-05-29 Cooperative journals: 《中国全科医学》
Abstract： Autologous platelet concentrate（APC）is a platelet concentrate obtained by centrifugation of human or animal blood，mainly includes first-generation platelet concentrate platelet-rich plasma（PRP）and second-generation platelet concentrate platelet-rich fibrin（PRF），which is widely applied in varies fields such as dental implants，bone defect repairing and burn plastic surgery because of its role in wound healing and tissue repair. In recent years，autologous platelet concentrate has been applied to improve ovarian function，promote the repair of endometrial hyperplasia，treat cervical and vulvo-related lesions，and has achieved significant efficacy. However，there is a lack of effective integration of PRP and PRF research results in the field of gynecology. This paper systematically and comprehensively review the preparation，biological properties and applications in various gynecological diseases，in order to to provide clinicians with new treatment ideas for gynecological diseases and reference for the further application of APC.
Subjects: Medicine, Pharmacy >> Preventive Medicine and Hygienics submitted time 2023-05-11 Cooperative journals: 《中国全科医学》
Abstract： Background Overweight/obese women are a growing population with high incidence of depression，anxiety and psychological abnormalities. Due to lack of effective responsive measures to emotions and behaviors，weight loss outcomes are often unsatisfactory in overweight/obese women with anxiety and depression. And less attention has been paid to weight loss outcomes and emotions in this group. Objective To explore the effect of daily self-weighing supported by online supervision on body composition and emotions in overweight/obese women with anxiety and depression. Methods A prospective，randomized，controlled study was conducted with 92 overweight/obese women with mild to moderate anxiety and depression voluntarily recruited from Beijing's Haidian District from October to December 2019. The subjects were randomized into an experimental group（n=46） and a control group（n=46） at a ratio of 1 ∶ 1，and treated with weight control interventions for three months：besides eating the appropriate food combinations with scientific and individualized guidance and excising rationally，the experimental group measured their body weight daily with online supervision from the special member of our research group and reported the data to the group，while the control group measured their body weight per month，which was collected by our research group through monthly telephone follow-up. Body composition measurement result，anxiety assessed using Self-Rating Anxiety Scale（SAS） and depression assessed using Self-Rating Depression Scale（SDS） were compared at baseline and three months after the intervention. Results All the participants completed the questionnaire assessment and follow-up. After the intervention，the control group had higher average body weight，BMI，body fat percentage and body fat as well as larger average visceral fat area than the experimental group（P<0.05）. Reduced body weight，BMI，body fat percentage，body fat and visceral fat area were seen in the experimental group after intervention（P<0.05）. The SAS score and SDS score in the experimental group were also lowered after intervention（P<0.05）. The average post-intervention scores of SAS and SDS in the experimental group were lower than those in the control group（P<0.05）. Conclusion In overweight/obese women with anxiety and depression，daily self-weighing with online supervision contributes to weight and fat loss and the improvement of anxiety and depression symptoms，which is a simple，effective and safe intervention measure.
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