Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2023-09-21
Abstract： Objective: To analyze the relationship between the expression levels of microRNA-155 (miR-155), suppressor of cytokine signaling 1 (SOCS1) and disease severity in patients with ulcerative colitis (UC). Methods: A total of 130 UC patients admitted to the Second Hospital Affiliated to Hebei North University from September 2021 to June 2023 were selected. According to the modified Mayo score system, 85 patients were divided into activity period group (≥3 points) and 45 patients were divided into remission period group (< 3 points); according to the modified Truelove and Witts classification criteria, the UC active stage patients were divided into mild 35 cases (mild group), moderate 30 cases (moderate group) and severe 20 cases (severe group). A total of 90 healthy individuals who underwent colonoscopy for physical examination or those who had normal colonoscopy results after single polypectomy and excluded other diseases were selected as the control group. During colonoscopy, the colonic mucosal tissues of UC patients with significant lesions and the colonic mucosal tissues of control group 20 cm away from the anus were collected. The miR-155 and SOCS1 mRNA expression levels in tissues were determined by fluorescence quantitative PCR; the expression of SOCS1 protein in tissues was determined by immunohistochemistry. The miR-155 and SOCS1 mRNA and protein expressions in control group, remission period group and activity period group were compared. The miR-155, SOCS1 mRNA expression levels and the modified Mayo score in patients with different severity of UC activity period were compared. The correlation between miR-155, SOCS1 mRNA, and modified Mayo score in colonic mucosal tissue of UC patients, factors affecting the occurrence of severe illness in UC active phase patients, and the predictive value of miR-155, SOCS1 mRNA expression levels on the occurrence of severe illness in UC active phase patients were analyzed. Results: Compared with control group and remission period group, the expression level of miR-155 in the colonic mucosal tissues of the activity period group was significantly increased, and the expression level of SOCS1 mRNA and the positive expression rate of SOCS1 protein were significantly decreased (P<0.05). The expression level of miR-155 and modified Mayo score in colonic mucosal tissues of patients with UC activity in mild, moderate and severe groups were increased successively, while the expression level of SOCS1 mRNA was decreased successively (P<0.05). There was a negative correlation between miR-155 and SOCS1 mRNA expression levels in colonic mucosal tissues of UC patients (P<0.05); miR-155 was positively correlated with the modified Mayo score (P<0.05), while SOCS1 mRNA was negatively correlated with the modified Mayo score (P<0.05). High level of miR-155, low level of SOCS1 mRNA and high level of modified Mayo score were all risk factors for severe disease in UC active patients (P<0.05). The area under curve (AUC) predicted by the combination of the two for severe illness in UC patients during the active phase was 0.920, which was significantly higher than the AUC predicted by miR-155 and SOCS1 mRNA alone (0.834, 0.851) (P<0.05). Conclusion: The expression of miR-155 was abnormally high and SOCS1 mRNA was abnormally low in colonic mucosal tissues of patients with UC activity, both of them are closely related to the severity of UC disease, and have high predictive efficacy for the occurrence of severe disease in UC active stage patients.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Botany >> Applied botany submitted time 2023-09-20 Cooperative journals: 《广西植物》
Abstract： Saxifraga L. is rich in species diversity with about 440–500 species and broad distribution. The taxonomical issues of this genus have been continuously concerned by the botanists. Saxifraga has important ornamental and medicinal values. Some of European countries have made great efforts on the exploitation and utilization of its ornamental resources. China is one of the diversity centers of Saxifraga, but it has been lagged far behind Europe, USA and even Japan in exploitation and utilization on ornamental value of this genus. The world breeding work in Saxifraga started 150 years ago. By the end of 2022, the 1 692 names of cultivars had been on-line listed by The Saxifrage Society, but only one came from China. In this paper, the germplasm resources of Saxifraga, and progress on its classification and breeding were reviewed, and also the utilization of its ornamental resources was briefly introduced, which will provide an important reference for taxonomical research, breeding and horticultural application of this genus in China. The results were as follows: (1) Saxifraga is rich in germplasms, but many scientific issues remain in the evolutionary relationships of its subordinate systems, which require a systematic and in-depth investigation by integrating morphological and molecular biology methods. (2) The cultivars of this genus are mainly raised through cross-breeding and mutant selection, and the majority of cultivars produced from the UK, Czech Republic, Germany, and the Netherlands. (3) The breeding of Saxifraga started very late in China, with only few cultivars from one breeding method.
Subjects: Materials Science >> Materials Science (General) submitted time 2023-03-31 Cooperative journals: 《材料研究学报》
Abstract： High speed steels with high V content (HVHSS, 10 mass % of V) were fabricated by a process of pressing plus super solid-liquid phase sintering (SLPS) with atomized alloy powder as raw material. The effect of sintering parameters such as sintering temperature and holding time on densification, microstructure evolution as well as mechanical properties were systematically investigated, and the composition, morphology and distribution of the phases existed in the alloy were carefully analyzed. The results show that sintering temperature is the most important parameter affecting the performance of the sintered alloys, however holding time shows the main effect on the precipitation and evolution of carbides. The matrix of the as prepared HVHSS consists of acicular martensite and retained austensite, and there are three types of carbides, i.e. VC, complex molybdenum carbide and complex chromium carbide. Small spherical VC particles mainly distribute in the grains and along their boundaries. As sintering temperature and holding time increased, not only grains and carbides gradually coarsened but also more and more carbides precipitated. However, the precipitation of complex carbides deteriorates the alloy’s strength and toughness due to their poor morphology which cause serious stress concentration or forming carbide network along grain boundaries. The HVHSS possess high performance, such as hardness HRC 65-68, impact toughness and bending strength over 6 J/cm2 and 1800 MPa respectively.
Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2022-07-26 Cooperative journals: 《中国全科医学》
Abstract： Alport syndrome (AS) is one of the important causes of chronic kidney disease and end-stage renal disease. It is the second most common inherited kidney disease after autosomal dominant polycystic kidney disease. Autosomal dominant inheritance is a very rare mode of inheritance in AS. It has been reported that patients with autosomal dominant Alport syndrome (ADAS) progress to end-stage renal disease at a later age. This article summarizes the clinical and pathological features, as well as genetic mutations of a novel COL4A4 gene mutation c.3506-3528del (p.G1169Efs*13) in an ADAS family. One family member developed end-stage renal disease at the age of 31.
Subjects: Psychology >> Physiological Psychology submitted time 2021-07-27
Abstract： Disgust is an important basic emotion for human beings and animals, and it derives from distasteful oral responses to bitter (toxic) tastants, often accompanied with nausea and vomiting and a strong desire to stay away from the induced stimulus, and it has the function of avoiding potential disease threats. A large number of studies have shown that oxytocin, progesterone, and estrogens can affect perception, generation and expression of core disgust, as well as learning conditioned disgust and recognizing facial expression in varying degrees. These three hormones mainly affect the processing of disgust by modulating neurotransmitter receptors including serotonin, γ-aminobutyric acid, acetylcholine, and glutamic acid receptors, and thus affecting the activities of amygdala, insula, anterior cingulate gyrus, putamen, piriform cortex, and middle frontal gyrus. Future studies should explore the effects of these hormones on disgust in different sensory channels and also consider their moderating roles in different genders by accurately measuring hormone levels and controlling the task difficulties. In addition, researchers can combine neuroimaging technologies with behavioral studies to clarify the neuroendocrine mechanism of these hormones affecting disgust processing.
Subjects: Biology >> Botany >> Applied botany submitted time 2020-08-02 Cooperative journals: 《广西植物》
Abstract：水花生(Alternanthera philoxeroides)因其表型可塑性、高生长速率和快速无性繁殖能适应水、陆生境。利用光学显微镜和荧光显微镜对水、陆生境的水花生不定根、茎解剖结构、组织化学特征及质外体通透性进行研究。结果表明：（1）水生境下，其不定根皮层中具较大裂生型通气组织，无次生生长，内皮层具凯氏带且栓质化，皮层和皮下层明显木质化。（2）在陆生环境下，其不定根有次生生长，胞间具通气组织，内皮层具凯氏带且栓质化，皮层和皮下层略木质化；此外，不定根还具额外形成层，产生次生维管束，薄壁组织和不定芽，多年生不定根中具直接分裂的薄壁组织，周皮具凯氏带，且栓质化和木质化。（3）水、陆生境下，其匍匐茎具髓和中空髓腔，发生次生生长，具裂-溶生型通气组织、单层内皮层、厚角组织和木质化且栓质化的角质层，陆生匍匐茎周皮栓质化且木质化。（4）水花生质外体屏障结构组成复杂，黄连素无法穿透质外体屏障结构。水花生的上述解剖学特征，是水花生适应水、陆生境的有力证据。
Subjects: Geosciences >> Other Disciplines of Geosciences submitted time 2019-09-11 Cooperative journals: 《干旱区研究》
Abstract：丛枝菌根(Arbuscular mycorrhizas，AM)真菌是土壤生态系统中广泛分布的优秀微生物资源，是具有重要经济价值的药用植物。本文基于人工培育条件下对中药材红花(Carthamus tinctorius)分别进行摩西球囊霉(Glomus mosseae)、根内球囊霉(Glomus intraradices)和混合菌(G.mosseae，Glomus etunicatum，Glomus microagregatum，Glomus caledonium，Glomus cladoideum和G.intraradices) 3种AM真菌接种处理，并以接种等量灭活菌种作为对照组处理。采用高效液相色谱法测定不同采摘时期红花绒中羟基红花黄色素A和山奈素含量的变化。结果表明：与对照组相比，AM真菌接种处理显著促进了红花生物量的积累，且AM真菌混合接种效果显著高于AM真菌单接种；在次生代谢产物合成方面，AM真菌接种处理以及不同采摘期的红花中羟基红花黄色素A和山奈素的含量差异不显著。表明AM真菌虽能促进红花的生长，但不影响羟基红花黄色素A和山奈素的合成。
Subjects: Dynamic and Electric Engineering >> Electrical Engineering submitted time 2019-03-05 Cooperative journals: 《电气工程学报》
Abstract：高压直流电缆及附件稳态电场分布主要取决于绝缘材料的电导率，而电导率又与场强、温度紧密相关，这使得直流电缆附件电场分布相比高压交流电缆附件更复杂。为此，在固定电缆终端 XLPE 绝缘、硅油电导率和温度梯度的条件下，本文采用多物理场耦合软件仿真研究了应力锥体增强绝缘非线性属性对高压直流电缆户外复合型终端稳态电场分布的影响规律。仿真研究结果表明：复合型户外高压直流电缆终端，工厂绝缘和增强绝缘界面切向电场在应力锥体根部和顶部可能出现极大值；而在半导电应力锥内表面电场也可能在根部和顶部出现极大值；通过调控增强绝缘材料的非线性属性可实现复合型电缆终端电场分布的综合调控。
Subjects: Biology >> Zoology submitted time 2018-12-25 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究青贮组合型全混合日粮对育肥肉牛生长性能及血液指标的影响。选取体况良好、体重[(440.5±3.5) kg]相近的西门塔尔公牛30头，随机分为2组，每组15头牛。对照组（C组）饲喂单一青贮型全混合日粮，试验组（T组）饲喂青贮组合型全混合日粮。试验期180 d。结果表明：1)第1~60天、第61~120天和第121~180天，T组的平均日采食量均显著低于C组（P≤0.05），T组的平均日增重均高于C组（P＞0.05）；第61~120天和第121~180天，T组的料重比均显著低于C组（P0.05)。4）第60天、第120天，C组的血浆尿素氮(UN)含量显著低于T组（P<0.05）；第60天，C组的血浆总胆固醇(T-CHO)含量显著低于T组（P<0.05）；第120天、第180天，C组的血浆谷草转氨酶(AST)活性显著高于T组（P≤0.05）；第180天，C组的血浆谷丙转氨酶（ALT）活性显著高于T组（P<0.05）。综上，青贮组合型全混合日粮对育肥肉牛机体抗氧化功能和健康状况无不良影响，而且可在一定程度上改善机体代谢，并可提高育肥肉牛的生长性能。
Subjects: Biology >> Zoology submitted time 2018-12-24 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究饲用甜高粱裹包青贮料对肉羊生产性能及血清指标的影响，以评价饲用甜高粱裹包青贮料对肉羊的饲喂效果。试验选取3～4月龄小尾寒羊[（25.70±1.50） kg]和杜泊羊[（33.40±1.68） kg]母羊各14只作为供试动物，根据饲粮中粗饲料不同将其分为杜泊羊甜高粱青贮组(DG组，n=7)、杜泊羊玉米青贮组(DY组，n=7)、小尾寒羊甜高粱青贮组(HG组，n=7)和小尾寒羊玉米青贮组(HY组，n=7)。饲养试验为期3个月，并于试验第30天、第60天和第90天早晨称重及采集血液。结果表明：1）玉米青贮组（DY组+HY组，n=14）的平均日采食量（ADFI）和平均日增重（ADG）极显著高于甜高粱青贮组（DG组+HG组，n=14）（P<0.01）。2）试验期内杜泊羊组（DG组+DY组，n=14）的血清总抗氧化能力(T-AOC)显著高于小尾寒羊组（HG组+HY组，n=14）（P<0.05）；在试验第60天，杜泊羊组血清丙二醛（MDA）含量显著低于小尾寒羊组（P<0.05）；在试验第90天，甜高粱青贮组的血清超氧化物歧化酶（SOD）活性显著高于玉米青贮组（P<0.05）；在试验第90天，HY组的血清MDA含量显著高于DY组和HG组（P<0.05）；甜高粱青贮组的血清SOD活性在试验第60天你和第90天显著高于试验第30天（P<0.05）。3）血清尿素氮(UN)含量以及谷丙转氨酶(GPT)、碱性磷酸酶(AKP)活性分别在试验第60天、第90天、第60天存在显著的品种效应（P<0.05）；血清β-羟丁酸（BHBA）含量及谷草转氨酶（GOT）、AKP活性分别在试验第90天、第30天与第90天、第60天存在显著的处理效应（P<0.05）；HY组的血清GOT和AKP活性在试验第90天显著高于试验第30天（P<0.05）。综上，饲喂含玉米裹包青贮料饲粮的肉羊的生产性能优于饲喂含饲用甜高粱裹包青贮料饲粮的肉羊，但青贮料长期育肥肉羊存在安全风险，而饲用甜高粱裹包青贮料较玉米裹包青贮料有利于提高肉羊肝脏健康、降低“高血脂”及机体酮症酸中毒等疾病的几率，进而降低安全风险。
Subjects: Materials Science >> Materials Science (General) submitted time 2017-11-21 Cooperative journals: 《金属学报》
Abstract：应用锌盐与氨水以及单氰胺水溶液间配体交换反应，制备了氰胺锌(ZnNCN)颗粒。并通过在前驱体盐溶液中共混银盐，利用相同的配体交换反应过程，制备了氰胺银(Ag2NCN)/ZnNCN复合颗粒。利用XRD、SEM、红外光谱（FT-IR）和紫外-可见（UV-Vis）吸收光谱对光催化剂的结构进行表征。结果表明，单一ZnNCN为花瓣状颗粒，宽禁带半导体材料(Eg=4.66 eV)。Ag2NCN/ZnNCN复合颗粒形貌与单一ZnNCN和Ag2NCN相比较有很大变化，两种金属氰胺化物以弱的物理作用力结合形成异质结构，复合颗粒的光谱响应范围扩展至可见光区，禁带宽度Eg=2.05 eV。以罗丹明B为光催化降解对象，研究了ZnNCN、Ag2NCN/ZnNCN复合颗粒在氙灯激发下的光催化活性。与单一ZnNCN以及Ag2NCN、ZnNCN机械混合物相比，Ag2NCN/ZnNCN复合颗粒表现出增强的光催化性能，表现为一级反应动力学特征。
Subjects: Biology >> Zoology submitted time 2017-10-11 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在适当降低饲粮氮水平条件下，通过饲粮添加N-氨甲酰谷氨酸（NCG）及小肠灌注葡萄糖，研究其对绒山羊小肠感应因子mRNA相对表达量、血液理化指标及激素含量的影响。选用27只体况良好、装有永久性瘤胃和十二指肠瘘管的内蒙古白绒山羊羯羊，按年龄和体重相近原则随机分成9组，每组3只。饲粮分为3个处理：低氮[粗蛋白质(CP)10.5%]、低氮+NCG（0.20 g/d）和高氮（CP 13.5%）；每个处理的山羊分别进行3个水平的十二指肠葡萄糖灌注：0、20和40 g/d。饲养试验（15 d预试期、15 d正试期）结束后，屠宰取空肠和十二指肠组织，通过实时定量PCR法测定营养感应因子mRNA相对表达量，测定血液理化指标、血清和空肠相关激素含量。结果表明：1）在基础饲粮条件（无葡萄糖灌注）下，随着饲粮氮水平的下降，空肠和十二指肠钠-葡萄糖共转运载体1（SGLT1）的mRNA相对表达量，血浆尿素氮、葡萄糖含量，血清瓜氨酸、胰岛素含量，血清和空肠胰高血糖素样肽1（GLP-1）、胰高血糖素样肽2（GLP-2）和促胰岛素肽（GIP）含量减少，而空肠和十二指肠溶质载体家族7成员9（SLC7A9）、溶质载体家族7成员1（SLC7A1）的mRNA相对表达量增加。2）增加适宜过瘤胃葡萄糖后，随着饲粮氮水平的下降，SGLT1、味觉受体1型1、味觉受体1型2、味觉受体1型3 mRNA相对表达量呈增加趋势。3）低氮饲粮条件下灌注20 g/d葡萄糖,额外饲喂NCG能够缓解饲粮氮水平降低引起的空肠和十二指肠SGLT1 mRNA相对表达量，血浆尿素氮、葡萄糖含量，血清瓜氨酸含量，血清和空肠GLP-1、GLP-2和GIP含量的下降。结果提示，适当降低饲粮氮水平，补饲NCG和增加过瘤胃葡萄糖（十二指肠灌注20 g/d）对绒山羊机体代谢及肠道营养物质感应均有促进作用。
Subjects: Biology >> Bioengineering submitted time 2017-07-24 Cooperative journals: 《中国生物工程杂志》
Abstract： Objective: The XylR-Pu is a classic toluene catabolic pathway, which is from TOL plasmid of Pseudomonas putida. In the presence of toluene, the XylR regulatory protein can activate Pu promoter and thus induce expression of corresponding metabolic genes. To detect 2,4,6-trinitrotoluene (TNT),the significant environmental pollutant, the pathway was optimized and put into Escherichia coli to construct whole-cell biosensor, which was based on the idea of synthetic biology. E.coli was chosen as chassis cell due to its genetic background was clear and it was simple to operate. Methods: pETDuet-1 was used as backbone to construct gene circuit of XylR-Pu, XylR was inserted in first multi cloning site . The second T7 promoter was substituted by Pu promoter and reporter gene of green fluorescent protein was under the control of Pu promoter . The fluorescent values can indicate the strength of the activation of XylR protein to Pu promoter. Then four series terminator was inserted between XylR and Pu to minimize background expression. Finally, the receptor domain of XylR protein was randomly mutated using sequential error prone PCR to construct a mutant library and to identify XylR mutants, which can be more sensitive and specific to TNT. Results: The four series terminator can effectively prevent read-through and decrease background fluorescent. After selection, one mutant protein named eX0-4 displayed better induction intensity, sensitivity and specificity to TNT. Conclusions: As Nitrobenzene was not XylR natural inducer, so XylR showed no obvious response to TNT. But the method is feasible to modify the A domain of XylR protein to obtain non-natural but better protein components. The mutant of eX0-4 enriched the reservoir of TNT-sensing elements, and provided a more applicable toolkit to be applied in genetic routes and live systems of biosensors in future. It can be a common method to identify biological elements to use error prone PCR to construct mutant library.
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