Subjects: Medicine, Pharmacy >> Preventive Medicine and Hygienics submitted time 2023-03-28 Cooperative journals: 《中国全科医学》
ZHANG, Guoqing
TONG, Tingting
WANG, Ying
LI, Kuiwu
ZHANG, Lida
WU, Xiaoqing
LI, Chenglong
WANG, Junli
ZHANG, Junyu
HAN, Wei
Abstract： Background The incidence，mortality and disability rates of ischemic stroke are high，and the reperfusion injury after thrombolytic therapy has a great impact on patients. Acupuncture is a characteristic therapy for the treatment of the disease，but the action mechanism remains unclear. Objective To observe the effect of Tongdu Tiaoshen electroacupuncture （EA） pretreatment on miR-124-3p/glycogen synthase kinase β（GSK-3β）/cyclin D （Cyp-D）signaling pathway and mitochondrial permeability transition pore of cerebral ischemia-reperfusion injury （CIRI） rats，and explore its possible mechanism of prevention and control of CIRI. Methods June to August 2022，a total of 100 clean SD rats were randomly divided into the sham operation group，model group，EA group，agonist group and EA + inhibitor group，with 20 rats in each group. For 7 d of intervention before modeling，in the EA group and EA + inhibitor group，"Baihui"（GV 20），"Fengfu"（GV 16）and "Dazhui"（GV 14）were selected to perform electroacupuncture 1 time a day for 7 days. For 24h before modeling，miR-124 agonist and inhibitor （5 nmol） were injected into the lateral ventricles in the EA + agonist group and inhibitor group. Except for the sham operation group，the right cerebral ischemia-reperfusion model of rats was prepared by the modified suture method in the rest groups. The right cerebral cortex of rats was taken，the degree of neurological impairment in each group was observed using mNSS scale and TTC staining，the nerve cell injury was observed by TUNEL staining and transmission electron microscopy. The expressions of GSK-3β，p-GSK-3β，Cyp-D，Cyt-C and Caspase-3 in cerebral cortex of each group were detected by immunofluorescence staining，Western blot and real-time quantitative PCR. Results Except for the sham operation group，all rest groups of rats were successfully modeled. Compared with the sham operation group，mNSS score and infarct volume of brain tissue were increased，mitochondrial structure was seriously damaged，cell apoptosis index was increased，p-GSK-3β positive expression was reduced，Cyp-D positive expression was enhanced，miR-124-3p，Cyp-D，Cyt-C and Caspase-3 mRNA expressions were increased，p-GSK-3β/ GSK-3β ratio was decreased，relative Cyp-D，Cyt-C，and Caspase-3 protein expressions were increased，and MPTP openness degree was increased in the model group（P<0.05）.Compared with the model group，mNSS score and relative infarct volume were decreased，mitochondrial structure destruction was relieved，p-GSK-3β/GSK-3β ratio was increased，and relative expression of Caspase-3 protein was decreased in the electroacupuncture group and inhibitor group；the mRNA expression of GSK-3βwas increased in electroacupuncture + agonist group（P<0.05）；apoptosis index was decreased，positive expression of p-GSK-3β was reduced and positive expression of Cyp-D was enhanced respectively，miR-124-3p，Cyp-D，Cyt-C and Caspase-3 mRNA expressions were decreased，Cyp-D and Cyt-C protein expressions were decreased，the degree of MPTP openness was decreased in the electroacupuncture group，inhibitor group and electroacupuncture + agonist group（P<0.05）. Compared with the electroacupuncture group，p-GSK-3β positive expression was enhanced，Cyp-D positive expression was reduced，miR-124-3p and Cyp-D，Cyt-C，Caspase-3 mRNA expressions were decreased，GSK-3β mRNA expression was increased，Cyp-D and Cyt-C protein expressions were decreased，and MPTP openness degree was reduced in the agonist group（P<0.05）；the apoptosis index was increased，the positive expression of P-GSK-3β was reduced，the positive expression of Cyp-D was enhanced，the expressions of miR-124-3p，Cyt-C and Caspase-3 mRNA were increased，the expressions of Cyp-D and Cyt-C protein were increased，and the openness degree of MPTP was increased in the electroacupuncture + agonist group（P<0.05）. Conclusion Tongdu Tiaoshen electroacupuncture pretreatment can alleviate neurological impairment in cerebral ischemia-reperfusion rats，the mechanism may be related to mediating miR-124-3p/GSK-3β/Cyp-D signaling pathway，inhibiting MPTP openness and thus reducing cell injury. The results of this study further verified the therapeutic effect of Tongdu Tiaoshen electroacupuncture in the treatment of CIRI from the mechanism，providing a new scientific basis for preventive treatment of disease of TCM and promote its clinical application.
Subjects: Other Disciplines >> Synthetic discipline submitted time 2023-03-28 Cooperative journals: 《中国科学院院刊》
Abstract： The Tibetan Plateau and surrounding mountains contain the largest glacier storage outsides the north and south polar regions. Under the global warming, recently glaciers in China are generally experiencing rapid melting and shrinkage. This rapid glacier change impacts the water resource that supplied by melt-water, and also favors glacial hazards, such as glacier collapse, glacier surging, glacial debris flow, and glacial lake outburst flood. Those glacial hazards show their spatio-temporal distribution characters, dynamic processes and mechanisms. Accompanying with the recent rapid glacier change, which reduces the glacier stability by warming, glacial hazards become more frequent. Furthermore, since global warming continues and human activity intensifies, the uncertainty and risk of glacial disasters will intensify. Therefore, scientific solution and countermeasures are needed based on the researches on glacial changes and hazards.
Subjects: Other Disciplines >> Synthetic discipline submitted time 2023-03-28 Cooperative journals: 《中国科学院院刊》
Abstract： The Tibetan Plateau lakes, with more than 50% area of the total lakes in China, are an important part of the Asia Water Tower. From 1970s to 2018, the number and area of lakes increased obviously, but the changing rate was not uniform. Before 1990, the negative balance of lake water storage was caused by low temperature inhibition of melting water. From 1990 to 2000, lake water storage increased associated with increased melting water due to the rising of temperature. After 2000, precipitation was the main factor leading to the increase of lake water storage, but the continuous temperature rising during 2005 and 2013 strengthened evaporation and weakened the increasing rate of lake water storage. Nevertheless, in the central and western Tibetan Plateau, the increase of lake water storage during 2000 and 2013 was mainly contributed by glacier melt water. From 1970s to 2013, the spatial characteristics of the lake water storage changes on the Tibetan Plateau were consistent with that of precipitation in the westerly and Indian monsoon regions. The increase of air temperature and long-wave radiation make the lake water temperature increase obviously, and promote the transmission efficiency of food chain. With the lake water storage increase, the lake water salinity generally decreases, which conduce the increase of lake biological diversity and make the lake ecosystem structure relatively complex. In the next 20 years, the lake water storage in the inland closed lakes of the Tibetan Plateau will continue to increase while the increasing rate will decrease. For the “Asian Water Tower”, the lake study on the Tibetan Plateau should focus on macroscale water storage and water balance, the main physical and chemical properties of lake water and ecosystem parameters, as well as the water cycle process of lake changes in large-scale climate change.
Subjects: Other Disciplines >> Synthetic discipline submitted time 2023-03-19 Cooperative journals: 《中国科学院院刊》
Abstract： The bio-medical data has entered a new era from exabyte-scale of genomic data to petabyte-scale of multi-dimensional big data, transforming the biological and medical research into a “data-intensive science” that is also referred as the fourth paradigm of discovery. Such transformation presented a set of new challenges: we have to efficiently gather and share high-dimensional and multi-level clinical and research data, further facilitate the comprehensive utilization of various omics data, clinical data, and phenome data of large population, eventually convert big data to new knowledge. Such challenges have to be faced by employing a new series of paradigm shifting ideas. In particular, new frameworks should be developed to improve the current submission-based data storage system to an integration-oriented system; to improve the subjective-based data sharing system to an interactive-oriented system; to integrate the cutting edge information technologies into the current data mining system. At the same time, large efforts have to be invested in developing data standardization guidelines and quality control technologies. These ideas will be critical in order to establish next generation of bio-medical big data centers and will be a new trend of future research.
Subjects: Biology >> Zoology submitted time 2018-12-24 Cooperative journals: 《动物营养学报》
Abstract：本研究通过测定棉秆部位比例及其概略养分、绵羊对棉秆整体消化率、绵羊对棉秆饲粮的自由采食量、消化代谢和饲喂棉秆饲粮对绵羊生长、屠宰性能、羊肉安全的影响，旨在对棉秆的饲料特性、营养价值与棉秆饲粮营养价值及饲喂棉秆饲粮的绵羊羊肉安全性进行综合评价。本研究中试验1测定棉秆不同部位比例及概略养分；试验2在限饲条件下，以差数法测定绵羊对棉秆的消化率；试验3采用自身对照设计，以玉米秸秆饲粮为对照，测定绵羊对棉秆饲粮的消化代谢；试验4通过120 d的饲喂试验，研究玉米秸秆饲粮和棉秆饲粮对绵羊生长及屠宰性能的影响，并测定棉秆组绵羊组织样中游离棉酚和主要农药残留含量。结果表明：棉秆中粗茎、细枝、叶和棉桃壳比例分别为31.48%、10.99%、17.12%和40.41%，棉秆的有机物、粗蛋白质、纤维素、半纤维素和木质素含量分别为90.00%、6.40%、34.80%、15.60%和10.30%。绵羊对棉秆干物质、有机物、粗蛋白质、纤维素、半纤维素、能量、钙和磷的（表观）消化率分别为38.20%、38.00%、1.58%、60.50%、58.10%、44.10%、57.80%和45.00%，消化能为10.28 MJ/kg DM。饲喂棉秆饲粮绵羊的有机物、纤维素、能量表观消化率比饲喂玉米秸秆饲粮分别低5.30%、10.00%和13.60%，钙、磷表观消化率分别高52.40%和36.70%，饲喂2种饲粮绵羊间的自由采食量和粗蛋白质表观消化率均无显著差异（P>0.05）。饲喂棉秆饲粮和玉米秸秆饲粮绵羊间的平均日增重、胴体重和胴体瘦肉重均无显著差异（P>0.05）。饲喂棉秆饲粮的绵羊组织样中游离棉酚及主要农药残留含量的检测结果为均未检出。本研究表明，棉秆中棉桃壳、细枝和叶3部分比例之和约70%，可采食、可利用部分资源量较大；棉秆是一种能量营养低于玉米秸秆、钙磷营养高于玉米秸秆的粗饲料，其木质素含量较高，粗蛋白质表观消化率极低（在生产中可计为0）；棉秆饲粮组与玉米秸秆饲粮组对绵羊增重、屠宰性能的影响无显著差异，二者的饲喂效果相当；饲喂棉秆饲粮的绵羊组织样中未检出游离棉酚及主要农药残留，其食品安全性未受到影响。
Subjects: Environmental Sciences, Resource Sciences >> Basic Disciplines of Environmental Science and Technology submitted time 2017-09-20 Cooperative journals: 《中国科学院院刊》
Abstract：西风与印度季风两大环流是控制青藏高原气候与环境变化的决定性因素。研究显示两大环流的影响范围和程度具有明显的空间分异：基于降水稳定同位素实测与模型模拟发现青藏高原现代西风与印度季风的相互作用特征表现为3种模态，即印度季风模态、西风模态和过渡模态。基于湖泊沉积记录的分析，发现3种模态主控范围在历史时期不断发生变化。3种模态对现代青藏高原环境产生连锁式环境效应，使得该区的冰川、湖泊、生态系统变化具有明显的区域特征，具体表现为印度季风模态的冰川强烈退缩，湖泊趋于萎缩；西风模态的冰川趋于稳定甚至部分出现前进，湖泊趋于扩张；过渡模态的冰川退缩程度减弱，湖泊变化不明显。西风模态的植被返青期提前，印度季风模态的植被返青期推后，而过渡模态的植被过程比较复杂。
Hits
Hits
Downloads
Comment