Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-07 Cooperative journals: 《分子影像学杂志》
Abstract： Objective To compare the clinical effectiveness of the combination of moist exposed burn ointment (MEBO) andsupportive care and skin barrier cream on the hand-foot syndrome(HFS) in patients treated with capecitabine. Methods A totalof 119 patients from Nanfang hospital were randomly divided into treatment group and control group. Patients in controlgroup were applied skin barrier cream and traditional skin care, while those of trentment group were treated with MEBO andsupportive care. Results Compared with the control group，patients of the treatment group had a lower severity and incidenceof HFS (P<0.05). It also had an effective ratio as well. Conclusion MEBO and supportive care decrease the severity andincidence of HFS caused by capectitabine more effectively compared with skin barrier cream.
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-07 Cooperative journals: 《分子影像学杂志》
Abstract： Objective To unravel the differences of biological characteristics and the uderlying molecular mechanisms betweendifferent passages of U87 glioblastoma cell line.Methods U87 (I) and U87 (II), with less or more passage number respectively,were established examined separately by MTT assay, transwell chamber assay, boyden chamber assay. Western blot was usedto analyze the expression of CTHRC1, FOXM1, PLOD2, MMP9, TGF-β, E-cadherin, Slug, Snail, Vimentin, PI3K, p-PI3K, Aktand p-Akt separately in these two types of cells.Results Compared to U87 (II), U87 (I) was more prone to form tubules andshowed more invasiveness, while there were no differences between U87 (I) and U87 (II) in proliferation and migration. Theexpression of markers of EMT varies in these two types of cells, with more expression of E-cadherin and Slug and lessexpression of Vimentin and Snail in U87 (II). Moreover, U87 (II) was found to have greater amount of Akt and p-Akt, but havesmaller amount of p-PI3k. No significant difference was found on the expression of PI3K between these cells. Additionally, theexpression of PLO2, CTHRC1 and MMP9 were higher in U87 (I) than that in U87 (II), while the expression of TGF-β in U87 (I)was lower than that in the latter one.Conclusion As the passage number increased, U87 cell lines exhibited changes ininvasiveness as well as some oncogenic genes’ expressions. It may lead to the different results during different periods, makingthe results inconvincible. Together, our results showed that, to lessen the influence of passage numbers on cells’ biologicalcharacteristics, scientists should use cells with same passage numbers and finish the experiments as quickly as they can.
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