Subjects: Medicine, Pharmacy >> Clinical Medicine Subjects: Biology >> Bioengineering Subjects: Physics >> Electromagnetism, Optics, Acoustics, Heat Transfer, Classical Mechanics, and Fluid Dynamics submitted time 2019-01-02
Abstract： Objective To investigate the effects of transcranial magnetoelectric stimulation (TMES) on temporal lobe epilepsy rats induced by kainic acid (KA). Methods 62 rats were divided into pretreatment (32 in total) and treatment (30 in total) groups according to the random number table method. The pretreatment group was further divided into 4 groups, and each group was stimulated by 0 %, 25%, 50%, 75% of the maximum current intensity (MCI) of the therapeutic apparatus respectively. According to the therapeutic efficacy, the optimal stimulation parameters under the experimental conditions was determined. The treatment group was further divided into 3 groups according to the random number table method, 10 in each group. Two groups (epilepsy-stimulating group, epilepsy-non-stimulating group) were epilepsy model rats that met the inclusion criteria. The stimulation parameters in the stimulating group were the best stimulation parameters explored in the pretreatment group; the rats in the non-stimulating group were treated the same before and after stimulation as the stimulating group. However, the therapeutic device has no effective energy output. The rats of third group difined as control were unmodeled control rats. All rats in the three groups were stimulated once a day for 40 minutes each for 14 days. The behavioral, histological and electrophysiological changes in the three groups of rats were recorded and compared to evaluate the efficacy of TMES therapy in epileptic rats. Results 50% MCI is the best stimulus intensity. The frequency of epileptic waves in epilepsy-stimulated rats was significantly lower than that in non-stimulated epileptic rats [(30.210 ± 4.580) beats/min vs. (31.380 ± 4.247) beats/min]. The difference was statistically significant (t = 3.235, P=0.001). The results of Timm staining showed that there was a statistically significant difference in the degree of staining between the three groups (F=17.429, P=0.000). The level of Timm staining in the inner molecular layer of dentate gyrus of the hippocampus in the epilepsy-stimulated group was significantly lower than that in the non-stimulated group, and the difference was statistically significant (P=0.027). Conclusion Transcranial magnetoelectric treatment can influence the formation of dentate gyrus neurons loop by improving epileptic rat dentate gyrus in epileptogenesis in changes of molecular layer organization degree, thereby reducing the frequency of epileptic EEG seizures.
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