Subjects: Biology >> Biochemistry Subjects: Biology >> Molecular Biology submitted time 2024-01-25
Abstract： Objective: To explore the possible effects of different sweet taste substances on the body by analyzing the changes of urinary proteome in mice after self-consumption of different sweet taste substances.
Methods: Urine samples of C57BL/6l mice were collected before and after self-consumption of sweet substances, including sucrose, stevia glycosides, acesulfame and sucralose, which are widely used in the world and can cause the preference reaction of mice. Among them, the concentration of non-nutritive sweeteners was selected as the concentration that has been shown to have the strongest preference reaction of mice. Label-free quantitative proteomics using high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for analysis. Differential proteins of urine proteome were screened in groups for analysis of protein functions and biological pathways. The urine proteome of single mice before and after self-consumption of sweet substances was compared, and the common differential proteins were counted; and the different sweet substances were compared horizontally.
Results and conclusions: Urine proteome can reflect the body changes of mice after self-consumption of sweet substances. And the effects of different sweet substances on urine proteome were different. Among the four sweet substances, sucralose caused the most similar changes in the body compared with sucrose. Compared with sucrose, stevia glycosides caused the most different changes in the body. The body changes caused by sucrose, acesulfame and sucralose are similar, but the body changes caused by stevia glycoside are different from other sweet substances. After self-consumption of the four sweet substances, the urine proteome differential proteins in mice all had proteins that had been reported to be related to brain reward circuitry, while only the urine proteome differential proteins after self-consumption of sucrose, acesulfame and sucralose were mainly related to metabolic processes. Urine proteomic differential proteins after acesulfame of stevia glycoside were mainly related to nucleosome assembly and gene expression.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Biochemistry Subjects: Biology >> Molecular Biology submitted time 2024-01-02
Abstract： Objective: Comparison of urine proteome between obese people and normal people.
Methods: Urine samples from obese and normal people were collected and identified by non-label quantitative proteomics using high performance liquid chromatography tandem mass spectrometry (LC-MS/MS). The difference proteins of urine proteome between obese and normal people were screened for protein function and biological pathway analysis. The urine proteome of obese individuals was compared with that of normal people, and the common differential proteins were counted to analyze the protein function and biological pathways. Reported biomarkers of obesity were searched in the urine proteome of obese individuals.
Results: 38 different proteins can be identified in the urine proteome of obese people compared with normal people, some of which have been reported to be related to metabolism and obesity, and the biological processes of differential proteins are also related to metabolism and other processes. 8 common differential proteins in the urine proteome of obese individuals and normal people, among which some proteins have been reported to be related to metabolism and obesity, and the biological processes of differential proteins are also related to metabolism and other processes. Among the differential proteins in the urine proteome of obese individuals compared with the normal people, the reported obesity biomarkers can be matched.
Conclusions: The urine proteome can distinguish the obese people, and the differential proteins in the urine proteome have key proteins that are known to be related to obesity and metabolism, and the biological processes of differential proteins also related biological processes such as nutrition and metabolism. Urine proteome has the potential to explore the pathogenesis of obesity and provide personalized treatment.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry Subjects: Biology >> Molecular Biology submitted time 2023-11-16
Abstract： Objective: To compare urine proteome changes in the first two days after mating behavior in male rats.
Methods: The urine samples of Sprague-Dawley rats on the day of mating and the day after mating were collected and analyzed by non-label quantitative proteomics by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the differential proteins (FC > 1.5 or < 0.67) in the urine proteome were screened. P<0.050) protein function and biological pathways were analyzed.
Results: 43 different proteins were identified in the urine proteome between the day after mating and the day after mating. By searching the Uniprot database and Pubmed database and related literature reports, nearly two-thirds of the differential proteins were associated with spermatogenesis.
Conclusions: The urine proteome changed the day after mating compared to the day after mating, and some of the known functions of the changed proteins were associated with spermatogenesis.
Peer Review Status:Awaiting Review
Subjects: Biology >> Molecular Biology submitted time 2023-05-05
Abstract： Urine proteome can reflect how short-term changes in growth and development of animal organisms and whether short-term developmental effects on urinary protein need to be considered when performing urine marker studies using animal models built with faster growing periods? In this study, urine samples were collected from 10 Wistar rats aged 6-8 weeks 3 and 6 days apart and analyzed using a non-labeled quantitative proteomics technique with high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS). The results showed that urine proteome could sensitively reflect the changes of short-term growth and development in rats. For example, comparing the urine proteome of Day0 and Day6, 195 differential proteins could be identified after screening (FC ≥ 1.5 or ≤ 0.67, P < 0.05), and verified by randomization, the average number of randomly generated differential proteins was 17.99, and at least 90.77% of differential proteins were not randomly generated. This demonstrates that the differential proteins identified by the different time points contrast are not randomly generated. According to differential protein GO analysis and KEGG pathway analysis, a large number of biological processes and signaling pathways related to growth and development were enriched, which provided the basis for urine proteome to reflect the short-term growth and development of rats, provided a means for in-depth and meticulous study of growth and development, and also provided an interfering factor that needs attention for animal experiments using 6-8-week-old rats to construct models. The results of this study demonstrated that the urinary proteome could see the difference of urinary protein in rats aged 6-8 weeks only 3-6 days apart, which broadened the sensitivity boundary of urinary proteomics and showed the sensitive and precise characterization ability of urinary proteome to body changes.
Peer Review Status:Awaiting Review
Subjects: Biology >> Molecular Biology submitted time 2020-02-20
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Peer Review Status:Awaiting Review
Subjects: Biology >> Molecular Biology submitted time 2018-12-10
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Peer Review Status:Awaiting Review
Subjects: Biology >> Molecular Biology submitted time 2018-08-29
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Peer Review Status:Awaiting Review
Subjects: Biology >> Molecular Biology submitted time 2018-05-14 Cooperative journals: 《广西植物》
Abstract：松萝酸是地衣中分布最广泛，含量最丰富的地衣酸之一，因早期作为抗生素而闻名于世。近20年来，有关松萝酸在抗菌、抗病毒、抗肿瘤等方面的活性作用机理不断有新的突破。研究揭示松萝酸主要通过影响细菌生物膜的形成、抑制细菌及病毒RNA和DNA的合成从而达到抑制细菌和病毒的目的，通过诱导肿瘤细胞程序性凋亡及抑制肿瘤细胞DNA的合成等途径，达到杀死肿瘤细胞的效果，并且松萝酸还具有抑制肿瘤细胞迁移的能力。但与此同时，松萝酸作为减肥营养补充剂而引发肝细胞线粒体功能障碍，进而导致肝脏损伤的作用也日益受到了科学界的关注。为更好的把握松萝酸资源研究开发的方向，本文对近20年来松萝酸在抗菌、抗病毒、抗肿瘤等活性方面的作用机理及肝脏毒方面的研究进行了归纳分析。截至目前，松萝酸在隶属于35个属的种里被报道有分布，其中大多数是枝状地衣，其次为叶状地衣，壳状地衣只有个别种里有。该文也对目前已报道的含有松萝酸的属种及有关影响松萝酸含量的环境因子的研究进行了全面的梳理，以期为富含松萝酸的优良种质资源的发掘和开展后续活性作用研究需要面对的松萝酸种质资源问题提供理论帮助。
Subjects: Biology >> Molecular Biology submitted time 2018-05-07 Cooperative journals: 《广西植物》
Abstract：物种遗传多样性与其适应能力和进化潜力密切相关，了解珍稀濒危植物遗传多样性和遗传结构可以为其保护策略与管理方式的制定提供理论基础。薄叶金花茶、小花金花茶和小瓣金花茶是三种濒危金花茶植物，本研究利用微卫星标记对它们的7个种群共184个个体进行遗传多样性和遗传结构分析。11个位点共检测到等位基因92个。在物种水平上，小瓣金花茶平均等位基因数（NA）为3.9、有效等位基因数（NE）为2.328、观测杂合度（Ho）为0.520、期望杂合度（He）为0.501，高于薄叶金花茶和小花金花茶。在种群水平上，有效等位基因数（NE）在1.788～2.466之间，期望杂合度（He）在0.379～0.543之间；种群间遗传分化系数（FST）在0.143 7～0.453 3之间，种群间基因流（Nm）在0.301 5～1.488 9之间。AMOVA 分子变异分析显示65.72%的变异存在于种群内。三种金花茶具有较低水平的遗传多样性和高水平的种群间遗传分化。STRUCTURE和PCoA种群遗传结构分析结果将取样种群分为2组，即薄叶金花茶和小花金花茶大部分个体分为一组，小瓣金花茶大部分个体分为一组。现存所有种群应根据实际情况尽快采取就地保护或迁地保护措施。
Subjects: Biology >> Molecular Biology Subjects: Biology >> Neurobiology Subjects: Biology >> Bioengineering Subjects: Physics >> Electromagnetism, Optics, Acoustics, Heat Transfer, Classical Mechanics, and Fluid Dynamics submitted time 2017-02-04
Abstract：是一种基于经颅磁电刺激技术有效治疗帕金森病、阿尔茨海默病的新观点，可理解成电压门控钙离子通道物理激活最佳靶点假说，基本内容：帕金森病、阿尔茨海默病等神经元退行性变疾病，与物理门控离子通道密切相关，可用物理手段来解决，激活递质能神经元是治疗的关键，电压门控钙离子通道则是物理手段激活的最佳靶点，目的是诱导钙离子内流触发神经元轴突终末突触囊泡释放神经递质。脑细胞激活论，指出了阿尔茨海默病、帕金森病等神经元退行性变疾病的治疗原则、方法与目的，提示了试图通过药物化学手段来寻求神经元退行性变疾病的解决办法，可能会动摇我们应对疾病的信心，而物理手段的应用或物理化学手段的联合应用也许是我们今后应对一些重大脑病的主要科研方向。
Peer Review Status:Awaiting Review
Subjects: Biology >> Cell Biology Subjects: Biology >> Neurobiology Subjects: Biology >> Molecular Biology Subjects: Physics >> Electromagnetism, Optics, Acoustics, Heat Transfer, Classical Mechanics, and Fluid Dynamics submitted time 2017-01-20
Abstract：This is a new idea that based on effective treatment of Parkinson's disease and Alzheimer's disease with transcranial magnetoelectric stimulation technology, it can understand a hypothesis about voltage-gated Ca2+ channels is the best target for activation by physical means, basic content：Parkinson's disease , Alzheimer's disease etc. neuronal degeneration diseases, that closely related to physical-gated ion channels, which can be treated with physical means, activating neurotransmitters-energic neurons plays key roles in the treatment, and voltage-gated Ca2+ channels is the best target for physical means, the purpose is to induce Ca2+ inflowing and triggers neuronal axon terminals synaptic vesicles releasing neurotransmitters. The theory of brain cell activation sets forth the principle, method and purpose of treatment of the physical gated ion channel diseases such as Alzheimer's disease, Parkinson's disease and other neural degeneration diseases, and indicates that the attempt to treat these diseases using pharmaceutical and chemical approaches could shake our confidence in conquering the diseases, and the application of physical approaches or combined application of physical and chemical approaches in the treatment of some major encephalopathy may be our main research direction in the future.
Peer Review Status:Awaiting Review
Subjects: Biology >> Molecular Biology Subjects: Biology >> Zoology submitted time 2016-10-06
Abstract： In this study, we introduced a general framework to use PacBio full-length transcriptome sequencing for the investigation of the fundamental problems in mitochondrial biology, e.g. genome arrangement, heteroplasmy, RNA processing and the regulation of transcription or replication. As a result, we produced the first full-length human mitochondrial transcriptome from the MCF7 cell line based on the PacBio platform and characterized the human mitochondrial transcriptome with more comprehensive and accurate information. The most important finding is two novel genes hsa-MDL1 and hsa-MDL1AS, which are encoded by the mitochondrial D-loop regions. We propose hsa-MDL1 and hsa-MDL1AS, as the precursors of transcription initiation RNAs (tiRNAs), belong to a novel class of long non-coding RNAs (lnRNAs), which is named as long tiRNAs (ltiRNAs). Based on the mitochondrial RNA processing model, the primary tiRNAs, precursors and mature tiRNAs could be discovered to completely reveal tiRNAs from their origins to functions. The ltiRNA/tiRNA system and their regulation mechanisms could exist ubiquitously in eukaryotes and prokaryotes. These findings will enrich the basic concepts in the field of mitochondrial biology, lnRNA functions and regulation of gene expression.
Peer Review Status:Awaiting Review
Subjects: Biology >> Molecular Biology submitted time 2016-06-16
Abstract： Iron-sulfur cluster proteins are a class of important mitochondrial functional proteins that play key roles in cellular energy metabolism, electron transport, substrate binding and activation, iron/sulfur storage, enzymatic reactions, and regulation of gene expression. Obstruction in the assembly and transport of iron-sulfur clusters will seriously affect the homeostasis of intracellular iron and the function of iron-sulfur proteins. Among them, IscA with a molecular weight of about 11-kDa is a highly conserved member of the iron-sulfur protein subfamily hesB. It can bind iron ions and [2Fe-2S] clusters, thereby participating in the synthesis of iron-sulfur cluster proteins, so it plays an important role in the iron-sulfur cluster assembly protein system. More noteworthy is that in 2015, Xie Can and Zhang Shengjia respectively The research group found that IscA1 has unique magnetic sensor (MagS) and magnetic receptor (MagR) functions. More importantly, it can regulate neural activity and behavioral localization by stimulating the expression of related magnetic genes through an external magnetic field. In view of the unique functions of magnetic receptors combined with related technologies, they are collectively referred to as magnetogenetics (magnetic genetics). This article briefly introduces the initial discovery and identification process of IscA protein, evolutionary conservation and its unique physiological and biological functions. , and condensed the mechanism regulation model of the magnetic genetic hypothesis.
Peer Review Status:Awaiting Review
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