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1. chinaXiv:201812.00002 [pdf]


Chen, Ze; Xu, Xiaofeng; Yang, Xiaojun; Dou, Weihao; Jin, Xiufeng; Ji, Haishuo; Liu, Guangyuan ; Luo, Jianxun; Yin, Hong ; Gao, Shan
Subjects: Biology >> Virology

本研究的主要目的是检测硬蜱携带的病毒。作为一个意外发现,检测到一个235 bp的来自非洲猪瘟病毒基因组的特异性片段。随后的测序发现,蜱和羊都具有这个片段,特别是共有一个特异性突变C38T,是目前已发布的非洲猪瘟病毒基因组上对应片段都没有的。

submitted time 2019-01-29 Hits5657Downloads2220 Comment 0

2. chinaXiv:201610.00002 [pdf]

Two novel genes discovered in human mitochondrial DNA using PacBio full-length transcriptome data

Subjects: Biology >> Molecular Biology

In this study, we introduced a general framework to use PacBio full-length transcriptome sequencing for the investigation of the fundamental problems in mitochondrial biology, e.g. genome arrangement, heteroplasmy, RNA processing and the regulation of transcription or replication. As a result, we produced the first full-length human mitochondrial transcriptome from the MCF7 cell line based on the PacBio platform and characterized the human mitochondrial transcriptome with more comprehensive and accurate information. The most important finding is two novel genes hsa-MDL1 and hsa-MDL1AS, which are encoded by the mitochondrial D-loop regions. We propose hsa-MDL1 and hsa-MDL1AS, as the precursors of transcription initiation RNAs (tiRNAs), belong to a novel class of long non-coding RNAs (lnRNAs), which is named as long tiRNAs (ltiRNAs). Based on the mitochondrial RNA processing model, the primary tiRNAs, precursors and mature tiRNAs could be discovered to completely reveal tiRNAs from their origins to functions. The ltiRNA/tiRNA system and their regulation mechanisms could exist ubiquitously in eukaryotes and prokaryotes. These findings will enrich the basic concepts in the field of mitochondrial biology, lnRNA functions and regulation of gene expression.

submitted time 2016-10-06 Hits15934Downloads2072 Comment 0

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