Subjects: Geosciences >> Geography submitted time 2024-04-29 Cooperative journals: 《干旱区研究》
Abstract： The Keluke Lake-Tuosu Lake Nature Reserve is a key wetland that represents the only large-scale stop-over site and an important habitat for migratory birds in the desert area of the Qaidam Basin, China. These birds migrate along the Central Asian-Indian migration route, which is part of the global migratory bird network. Most birds in the reserve are migratory birds. In this study, their diversity was investigated using a sample line survey and fixed points. A total of 18 families, 43 genera, and 138 species of birds were recorded, with 10 and 24 of the latter being listed as class I and class II wild species, respectively, which are protected nationwide in China. From 2019 to 2021, the number of bird species increased by 37%, with summer visitors and traveling birds increasing by 23.53% and 88.24%, respectively, which corresponded to their numbers increasing by 2.03 and 7.95 times. Summer visitors and traveling birds are important members of the bird community. The values of the Shannon-Wiener index of richness and the Pielou index of evenness for the bird community increased from 2.45 and 0.46 in 2019 to 3.64 and 0.63 in 2021, respectively. Bird diversity was shown to be high in the Keluke Lake-Tuosu Lake Nature Reserve, with the populations of black-necked cranes and red-crested pochards reaching the 1% population tandard established for Ramsar sites. Therefore, an application for the designation of the reserve as a Ramsar site needs to be made as soon as possible. Some individuals belonging to seven bird species, including red-crested pochard and common merganser among others, survived through winter in the Keluke Lake-Tuosu Lake Nature Reserve. Long-term bird diversity studies should be conducted in this area.
Subjects: Digital Publishing >> New Media submitted time 2023-10-08 Cooperative journals: 《中国传媒科技》
Abstract：随着社会科技的快速发展和居民生活水平的日益提高,人们对摄影技术提出了新要求与高标准。基于此,本文将阐释摄影大数据的内涵,分析大数据在摄影中的应用,阐述大数据在摄影中的发展,旨在促进大数据与摄影业的共同成长,推进摄影商业的蓬勃发展。
Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2023-03-01 Cooperative journals: 《中国全科医学》
Abstract： Background Klotho is closely related to the occurrence and development of kidney disease. Salt-sensitive hypertension（SSH）is often accompanied by kidney disease. At present，there are few reports on the role and molecules mechanism of klotho in renal injury in SSH. Objective To investigate the role and molecules mechanism of klotho in renal injury in SSH. Methods The rat glomerular mesangial cell line HBZY1 was selected as the experimental cells on June 15，2021， and the experimental cells were divided into the control group and the model group. The model of HBZY1 cell injury induced by NaCl 137 mmol/L and angiotensin Ⅱ（Ang Ⅱ）10-6 mmol/L was used to simulate the renal injury in SSH，and the cells were collected. The differences in the expression of klotho mRNA and protein were detected by real-time fluorescent quantitative PCR （qRT-PCR）and Western Blot. The interference vector and overexpression vector of klotho and the overexpression vector of angiotensin Ⅱ type 1 receptor（AT1R）were constructed. The klotho interference experiments were divided into five groups， including the control group，empty group，klotho-siRNA1 group，klotho-siRNA2 group and klotho-siRNA3 group； The klotho overexpression experiments were divided into three groups，including the control group，empty group and klotho overexpression group；The AT1R overexpression experiments were divided into three groups，including the control group，empty group and AT1R overexpression group. The constructed vectors were transfected into cells with verified transfection efficiency. After successful transfection，the experiment was divided into two parts. The first part of the experiment was to verify the renal protective effect of klotho，the experiment subjects were divided into four groups，including the control group，model group， klotho overexpression group and klotho interference group. The second part of the experiment was to explore whether the renal protective effect of klotho was related to AT1R，the experiment subjects were divided into three groups，including the model
group，klotho overexpression group and klotho+AT1R overexpression group. After successful transfection，the tests including cell viability detected by cell counting kit-8（CCK-8） method，reactive oxygen species（ROS）content detected by flow cytometry，malondialdehyde（MDA）and superoxide dismutase（SOD）content in cell supernatant detected by enzyme-linked immunosorbent assay（ELISA），interaction effect between kltho and AT1R detected by co-immunoprecipitation（Co-IP）. Results Compared with the control group，mRNA level and protein expression of klotho in the model group decreased in model group（ t=7.102，7.506； P=0.002，0.002），klotho-siRNA2 interference effect was more significant（ P<0.001）， the expression of klotho protein in the klotho overexpression group increased significantly（ P<0.001），the expression of AT1R protein in the overexpression group increased significantly（ P<0.001）. Effects of klotho on cell viability and oxidative stress injury：Compared with the control group，cell viability in the model group decreased（ P<0.001），intracellular ROS and MDA content increased（ P<0.001， P=0.004），and SOD content decreased（ P=0.041）；Compared with the model group，cell viability in the klotho overexpression group increased（ P<0.001），intracellular ROS and MDA content decreased and SOD content increased（ P<0.001， P=0.003， P=0.018）；Compared with the model group，cell viability in the klotho interference group decreased（ P<0.001），while intracellular ROS and MDA content increased and SOD content decreased（ P<0.001， P=0.002， P=0.001）. Effects of klotho on cell viability and oxidative stress injury through AT1R：Compared with the model group，cell viability increased（ P<0.001），intracellular ROS and MDA content decreased and SOD content increased（ P<0.001， P=0.024， P=0.007）in the klotho overexpression group；Compared with the klotho overexpression group，cell viability decreased（ P<0.001），ROS and MDA content increased and SOD content decreased（ P<0.001， P=0.001， P=0.002）in the klotho+AT1R overexpression group. Co-IP determined that there was an interaction between klotho and AT1R. Conclusion Klotho plays a protective role in renal injury in SSH by inhibiting oxidative stress injury through interaction with AT1R.
Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2023-01-28 Cooperative journals: 《中国全科医学》
Abstract：
Objective To investigate the role of Klotho in salt-sensitive hypertensive renal injury and its molecular mechanism. Methods HBZY1 cell injury model induced by NaCl 137 mmol/L and AngII 10-6 mmol/L was used to simulate salt-sensitive hypertensive kidney injury, and cells were collected. Real-time quantitative PCR (qRT-PCR) and Western Blot were used to detect the expression of Klotho mRNA and protein. The Klotho interference vector and overexpression vector were constructed, and the Angiotensinogen II receptor-1(AT1R) overexpression vector was transfected into cells. The transfection efficiency was verified by qRT-PCR and Western Blot. The following assays were performed after transfection. Cell proliferation activity was measured by CCK-8 assay, and ROS content was measured by Flow cytometry assay, the contents of malondialdehyde (MDA) and Superoxide dismutase superoxide dismutase (SOD) in the supernatant were detected by enzyme-linked immunosorbent assay (ELISA) , and the interaction between Klotho and AT1R was detected by co-immunoprecipitation (Co-IP) . Results Compared with the control group, the expression of Klotho mRNA and protein decreased in the model group (P<0.05). The KLOTHO-SIRNA2 interference effect was the best, and the expression of Klotho in the Klotho overexpression group and AT1R in the AT1R overexpression group increased, indicating the overexpression effect. Compared with the control group, the cell viability of the model group was decreased, the contents of ROS and MDA were increased, and the contents of SOD were decreased (P<0.05), the content of SOD decreased (P<0.05) , but the activity of klotho-treated cells decreased further, the content of ROS and MDA increased further, and the content of SOD decreased further (P<0.05). Compared with the model group, the Klotho overexpression group had higher cell viability, lower ROS and MDA contents and lower SOD contents (P<0.05) , while the Klotho + AT1R overexpression group had lower cell viability, higher ROS and MDA contents, the content of SOD decreased (P<0.05). CO-IP determines the interaction between Klotho and AT1R. Conclusion Klotho plays a protective role in salt-sensitive hypertensive renal injury by inhibiting oxidative stress injury through interaction with AT1R.
Subjects: Geosciences >> Geography submitted time 2019-01-11 Cooperative journals: 《干旱区地理》
Abstract：通过对克鲁伦河流域下游河水与地下水的主要离子水化学与氢氧同位素进行分析，结合区域水文地质资料，利用Durov图、空间插值、统计分析等方法分析了河水与地下水水化学与氢氧同位素特征。结果表明，河水的水化学类型主要为Na-Ca-HCO3型，地下水水化学类型为Na-Cl和Ca-Na-HCO型。克鲁伦河水主要离子浓度与氢氧同位素空间分布特征显著较地下水稳定、空间差异小。流域内地下水与地表水主要来自降水补给，地下水也是克鲁伦河的主要补给源。氘盈余变化揭示出克鲁伦河水的蒸发分馏程度强于地下水，除流域内水体蒸发主要受地质地貌影响外，人类活动对河水的影响显著于周边地下水。G3点所在的西庙为一个完整独立的水文地质单元，其表现出的水化学与氢氧同位素特征均异于其他地下水。流域内部分地下水F-含量超标，虽然一定程度是受人类活动影响，但更多的是基于综合水文地质条件基础上的自然现象，已严重威胁人类生存，应引起当地有关部门的高度重视，避免氟中毒事件重现。
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-01-25 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To explore the pattern of variations in middle cerebral artery peak systolic velocity (MCA PSV) and cardiothoracic ratio (CTR) during early pregnancy, establish their reference ranges and explore their correlation with the crown-rump length (CRL). Methods A total of 522 pregnant women with normal findings in antenatal examinations underwent routine color Doppler ultrasound examination to collect the data of MCAPSV, CTRand CRL. The reference ranges of MCA PSV and CTR for different CRL levels were established, and the correlation of MCA PSV and CTR with CRL was analyzed. Results During the first trimester, MCA PSV and CRL showed a moderate positive correlation with a correlation coefficient of 0.426 (P<0.001), while CTR and CRL showed no significant correlation (0.168, P<0.001). The reference range of MCAPSV was 14.35 (14.08-14.62) cm/s and that of CTRwas 0.34 (0.33-0.34) during early pregnancy. Conclusion Color Doppler ultrasound is a safe and feasible modality to assess fetal MCAPSV and CTR for detecting fetal growth abnormalities in early pregnancy. The established reference ranges of MCAPSV and CTR offer a clinical theoretical basis for detecting α-thalassemia inearlypregnancy.
Subjects: Biology >> Bioengineering submitted time 2017-11-26 Cooperative journals: 《中国生物工程杂志》
Abstract：猪支原体肺炎（Mycoplasmal pneumoniae of swine, MPS）是由猪肺炎支原体（Mycoplasma hyopneumonia, Mhp）引起的一种慢性呼吸道传染病，该病给养猪业造成巨大的经济损失。疫苗接种是目前减轻或预防由猪肺炎支原体感染引发经济损失的主要手段。本文对猪支原体肺炎基因工程疫苗近年来的研究进展进行综述，主要包括黏附因子相关疫苗、核苷酸还原酶相关疫苗、DNA疫苗、表达文库疫苗和肽疫苗等，最后对基因工程疫苗研究工作的继续开展提供了建议。
Subjects: Biology >> Bioengineering submitted time 2017-11-26 Cooperative journals: 《中国生物工程杂志》
Abstract：猪支原体肺炎（Mycoplasmal pneumoniae of swine, MPS）是由猪肺炎支原体（Mycoplasma hyopneumonia, Mhp）引起的一种慢性呼吸道传染病，该病给养猪业造成巨大的经济损失。疫苗接种是目前减轻或预防由猪肺炎支原体感染引发经济损失的主要手段。本文对猪支原体肺炎基因工程疫苗近年来的研究进展进行综述，主要包括黏附因子相关疫苗、核苷酸还原酶相关疫苗、DNA疫苗、表达文库疫苗和肽疫苗等，最后对基因工程疫苗研究工作的继续开展提供了建议。
Subjects: Biology >> Bioengineering submitted time 2017-11-21 Cooperative journals: 《中国生物工程杂志》
Abstract：猪支原体肺炎（Mycoplasmal pneumoniae of swine, MPS）是由猪肺炎支原体（Mycoplasma hyopneumonia, Mhp）引起的一种慢性呼吸道传染病，该病给养猪业造成巨大的经济损失。疫苗接种是目前减轻或预防由猪肺炎支原体感染引发经济损失的主要手段。本文对猪支原体肺炎基因工程疫苗近年来的研究进展进行综述，主要包括黏附因子相关疫苗、核苷酸还原酶相关疫苗、DNA疫苗、表达文库疫苗和肽疫苗等，最后对基因工程疫苗研究工作的继续开展提供了建议。
Subjects: Biology >> Bioengineering submitted time 2017-11-21 Cooperative journals: 《中国生物工程杂志》
Abstract：猪支原体肺炎（Mycoplasmal pneumoniae of swine, MPS）是由猪肺炎支原体（Mycoplasma hyopneumonia, Mhp）引起的一种慢性呼吸道传染病，该病给养猪业造成巨大的经济损失。疫苗接种是目前减轻或预防由猪肺炎支原体感染引发经济损失的主要手段。本文对猪支原体肺炎基因工程疫苗近年来的研究进展进行综述，主要包括黏附因子相关疫苗、核苷酸还原酶相关疫苗、DNA疫苗、表达文库疫苗和肽疫苗等，最后对基因工程疫苗研究工作的继续开展提供了建议。
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