摘要: The importance of the nitrate (
NO3−) transporter for yield and nitrogen-use efficiency (NUE) in rice was previously demonstrated using map-based cloning. In this study, we enhanced the expression of the
OsNRT2.1 gene, which encodes a high-affinity
NO3− transporter, using a ubiquitin (
Ubi) promoter and the
NO3−-inducible promoter of the
OsNAR2.1 gene to drive
OsNRT2.1 expression in transgenic rice plants. Transgenic lines expressing
pUbi:OsNRT2.1 or
pOsNAR2.1:OsNRT2.1 constructs exhibited the increased total biomass including yields of approximately 21% and 38% compared with wild-type (WT) plants. The agricultural NUE (ANUE) of the
pUbi:OsNRT2.1 lines decreased to 83% of that of the WT plants, while the ANUE of the
pOsNAR2.1:OsNRT2.1 lines increased to 128% of that of the WT plants. The dry matter transfer into grain decreased by 68% in the
pUbi:OsNRT2.1 lines and increased by 46% in the
pOsNAR2.1:OsNRT2.1 lines relative to the WT. The expression of
OsNRT2.1 in shoot and grain showed that
Ubi enhanced
OsNRT2.1 expression by 7.5-fold averagely and
OsNAR2.1 promoters increased by about 80% higher than the WT. Interestingly, we found that the
OsNAR2.1 was expressed higher in all the organs of
pUbi:OsNRT2.1 lines; however, for
pOsNAR2.1:OsNRT2.1 lines,
OsNAR2.1 expression was only increased in root, leaf sheaths and internodes. We show that increased expression of
OsNRT2.1, especially driven by
OsNAR2.1 promoter, can improve the yield and NUE in rice.
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