Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2022-12-23 Cooperative journals: 《中国全科医学》
Abstract：
Objective To explore the basic characteristics of gut flora in patients with esophageal squamous cell carcinoma.Methods 35 patients with esophageal squamous cell carcinoma without any anti-tumor treatment were included as esophageal cancer group (EC group), and 35 healthy volunteers as control group (DZ group). Collected stool samples of two groups people,then used 16S rDNA technology to detect intestinal flora, analyze the alpha diversity, beta diversity and differential flora species of the intestinal flora of two groups according to the species annotation of the test results. Results Shannon index, Simpson index, Chao1 index and ACE index of alpha diversity had no significant difference between the two groups (P<0.05), while principal coordinate analysis (PCoA) graph of the Beta diversity showed that the two groups were far apart on the whole, and the species results were different.T-test results of different species showed that the abundance of Faecalbacterium, Rosebria and Citrobacter in EC group decreased, while the abundance of Lactobacillus, Romboutsia, Ruminococcus_torques_group, Enterobacter and Turicibacter increased compared compare with DZ group at the genus level (P<0.05). LEfSe analysis showed that the abundance of g-faecalbacterium and s-faecalbacterium prausnitzii in EC group were significantly lower， and the abundance of g-Romboutsia and s-Romboutsia-ilealis increased significantly than that in DZ group at the genus and species levels(P<0.05).Conclusion Esophageal squamous cell carcinoma patients have significant differences in intestinal bacteria, among which faeculibacterium, faecalibacterium-prausnitzii,Rombutzia and Rombutsia-ilealis may be specific change species of esophageal cancer, which are closely related to the occurrence of esophageal cancer.
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