Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-27 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To observe the effect of rapid eye movement sleep deprivation (REMSD) for 5 days on depressive-like behavior and monoamine oxidase A (MAOA) expression in the amygdale of mice. Methods Adult male C57BL/6J mice were divided into blank control group, control group and REMSD group. REMSD models were established by a modified multiple small platform method. The mice were examined for locomotive activity in open field test (OFT) and for depressive-like behavior in forced swimming test (FST) and sucrose preference test (SPT) after treatment. After all the tests, the protein and mRNA expressions of MAOA in the amygdala were detected with Western blotting and real-time PCR, respectively. Results REMSD for 5 days significantly impaired the locomotive activity of the mice, which was obvious in 1 to 3 days after REMSD. The locomotive activity became normal on day 4 after the 5-day REMSD. The immobility time of the mice was lengthened in days 7 to 14 (P<0.01), and sucrose preference rate was reduced significantly in days 8 and 9 (P<0.01). The expression level of MAOA in the amygdala was increased significantly after the 5-day REMSD (P<0.01). Conclusion REMSD for 5 days causes delayed depressive-like behavior in mice possible in relation with the increased expression of MAOA in the amygdale.
Subjects: Biology >> Zoology submitted time 2017-10-23 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究不同相对湿度（RH）对间歇性26 ℃环境下肉鸡盲肠菌群多样性的影响。选取29日龄爱拔益加（AA）肉鸡180只转入环境控制舱，随机分成3个组（RH分别为30%、60%和85%），每组6个重复，每个重复10只鸡（公母各5只）。从29日龄开始，每天10：00—16：00(6 h)温度维持26 ℃，RH分别为30%、60%和85%，剩余时间温度为21 ℃，RH为60%。试验共14 d。采用16S rDNA的变性梯度凝胶电泳（DGGE）技术，结合特异性和共性条带割胶回收DNA进行克隆和测序，分析RH在间歇性26 ℃偏热处理第7和14天时对盲肠内容物菌群结构和多样性的影响。结果表明：1）试验第7天，30%RH组肉鸡盲肠DGGE条带数（菌群丰富程度）高于60%RH组，而85%RH组低于60%RH组；试验第14天，30%、85%RH组肉鸡盲肠DGGE条带数均高于60%RH组。2）聚类分析显示，试验第7天，85%RH对盲肠菌群影响明显；试验第14天，30%RH对盲肠菌群影响明显。但随着处理时间推移，30%RH对肉鸡盲肠菌群影响越大。3）间歇性26 ℃环境下不同RH组肉鸡盲肠内共性菌群是Faecalibacterium prausnitzii；在试验第7天，30%、85%RH组肉鸡盲肠中特异性菌群是Stomatobaculum longum。结果提示：间歇性26 ℃环境下，低湿(30%RH）和高湿（85%RH）影响肉鸡盲肠菌群的结构和多样性，且不同处理时间RH的影响不同。
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