Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2023-08-10 Cooperative journals: 《中国全科医学》
Abstract： Background As China enters the period of accelerated development of population aging， the proportion of rheumatoid arthritis cases is increasing，causing a serious burden on society and families. The active ingredients in simiao decoction have improved effect，but there is a lack of relevant studies to analyze the action and mechanism of prescriptions. Objective To observe the intervention effect of simiao decoction on rats with rheumatoid arthritis established by collagen#2;induced arthritis（CIA）and its effect on vitamin D system and neutrophil extracellular traps（NETs）. Methods From November 2021 to January 2023，70 SPF grade SD female rats aged 6 to 7 weeks were selected. The rats were randomly divided into the blank group（control group），model group（model group），methotrexate group（MTX group），vitamin D group（VD group），Simiao decoction low-dose group（SMT-L group），Simiao decoction medium-dose group（SMT-M group）and Simiao decoction high-dose group（SMT-H group）by random number table method，with 10 rats in each group. The rat model of synovial arthritis（CIA）was established by collagen induction method and the gavage treatment was started 7 d after modeling. The body mass and general condition of rats were observed before and after drug intervention，the degree of ankle swelling was assessed，and the arthritis index（AI）score of rats in each group was measured. Hematoxylin-eosin（HE）staining was used to observe the joint tissue lesions of rats. after drug intervention. The expressions of myeloperoxidase（MPO）and tumor necrosis factor α（TNF-α）in synovium tissue were detected by immunohistochemical method，and the levels of interleukin6（IL-6），25-hydroxyvitamin D〔25（OH）D3〕and NETs in serum of rats were detected by enzyme-linked immunosorbent assay（ELISA）. The expression levels of 24-hydroxylase（CYP24A1），1-α hydroxylase（CYP27B1），MPO，vitamin D receptor（VDR）and elastase（NE）in synovial tissue of joint were detected by Western Blot. Results The body mass of rats in the VD，SMT-L and SMT-H groups was higher than that in the model group on the 7th day of drug intervention，the body mass in the control，MTX，VD，SMT-L and SMT-H groups was higher than that in the model group on the 14th day， the body mass in the control， MTX， VD， SMT-L， SMT-M and SMT-H groups was higher than the model group on the 21st and 28th days，and the difference was statistically significant（P<0.05）. The degree of ankle swelling in the model，MTX，VD，SMT-L，SMT-M and SMT-H groups was higher than that in the control group on day 0 of drug intervention，the degree of ankle swelling in the control，MTX，VD，SMT-L，SMT-M and SMT-H groups was lower than that in the model group on day 7， the degree of ankle swelling in the model group was higher than the control group， the SMT-M group was higher than the model group on day 14， the degree of ankle swelling in the model group was higher than the control and MTX groups and lower than the VD，SMT-L，SMT-M and SMT-H groups on day 21， the degree of ankle swelling in the control， MTX， VD， SMT-L， SMT-M and SMT-H groups was higher than the model group， and the difference was statistically significant（P<0.05）. The AI score of rats in the SMT-H group was lower than that in the model group on day 14，the AI score in the MTX，VD，SMT-L and SMT-H groups was lower than that in the model group on day 21， the score of rats in the MTX，VD，SMT-L，SMT-M，and SMT-H groups was lower than that in the model group on day 28，the difference was statistically significant（P<0.05）. In the model group，the rat synovial tissue was infiltrated with a large number of inflammatory cells in the lower layer of synovial tissue lining， synovial cells were abnormally hyperplasia，articular surface was destroyed，and fibrous tissue hyperplasia occurred in different degrees. Compared with the model group，the pathological status of synovial tissue of rats in all drug administration groups was improved，especially in the MTX and SMT-H groups. Immunohistochemical results showed significant differences in the expression levels of MPO and TNF-α in synovial tissue among the 7 groups（P<0.05），MPO and TNF-α in the model group were higher than those in the control group，while MPO and TNF-α in the MTX，VD and SMT-H groups were lower than those in the model group， TNF-α in the SMT-M group was lower than that in the model group，and the difference was statistically significant（P<0.05）. IL-6 and NETs in the model group were higher than those in the control group，and 25（OH）D3 was lower than that in the control group. IL-6 and NETs in the MTX，VD and SMT-H groups were lower than those in the model group，and 25（OH）D3 was higher than that in the model group. NETs in the SMT-M group were lower than those in the model group，and the difference was statistically significant（P<0.05）. CYP24A1 in the model group was higher than that in the control，MTX，VD，SMT-M and SMT-H groups，while CYP27B1 and VDR in the model group were lower than those in the control，MTX，VD，SMT-M and SMT-H groups， MPO was higher than that of the control，MTX，VD，SMT-L，SMT-M and SMT-H groups，and NE was higher than the control，VD，SMT-M and SMT-H groups，and the difference was statistically significant（P<0.05）. Conclusion Simiao decoction may inhibit the formation of NETs by regulating the vitamin D system，thereby treating RA.
Subjects: Biology >> Zoology submitted time 2018-12-24 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在借助开路式呼吸测热系统研究饲粮非纤维性碳水化合物（NFC）/中性洗涤纤维/（NDF）对生长期杜寒杂交母羊生长性能、营养物质表观消化率及甲烷产量的影响。采用单因素试验设计，选取体况良好、体重为（27.8±0.5） kg的母羊30只，根据体重一致原则，分配到3组，各组饲粮NFC/NDF分别为0.78（精粗比为35:65）、1.03（精粗比为50:50）、2.17（精粗比为65:35组），每组10只羊。试验期为25 d，包括3 d调整期、7 d预试期和15 d正试期。结果表明：在NFC/NDF=0.78组自由采食和其他组限饲条件下，3组试验羊的初始体重和结束体重以及平均日增重无显著性差异（P>0.05）。当饲粮NFC/NDF由0.78增加至2.17时，干物质采食量显著降低（P0.05）。当饲粮NFC/NDF由0.78增加至2.17时，甲烷能、单位干物质采食量的甲烷产量、单位总能摄入量的甲烷能产量显著降低（P<0.05）。结果提示，在平均日增重一致的前提下，高NFC/NDF饲粮料重比较低，且甲烷转化效率较低；对生长期杜寒杂交母羊来说，限饲条件下饲喂NFC/NDF为2.17的饲粮在提高生产效益的同时又兼顾甲烷减排，效果相对最佳。
Subjects: Biology >> Zoology submitted time 2018-12-24 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在借助开路式呼吸测热系统研究饲粮非纤维性碳水化合物（NFC）/中性洗涤纤维/（NDF）对生长期杜寒杂交母羊生长性能、营养物质表观消化率及甲烷产量的影响。采用单因素试验设计，选取体况良好、体重为（27.8±0.5） kg的母羊30只，根据体重一致原则，分配到3组，各组饲粮NFC/NDF分别为0.78（精粗比为35:65）、1.03（精粗比为50:50）、2.17（精粗比为65:35组），每组10只羊。试验期为25 d，包括3 d调整期、7 d预试期和15 d正试期。结果表明：在NFC/NDF=0.78组自由采食和其他组限饲条件下，3组试验羊的初始体重和结束体重以及平均日增重无显著性差异（P>0.05）。当饲粮NFC/NDF由0.78增加至2.17时，干物质采食量显著降低（P0.05）。当饲粮NFC/NDF由0.78增加至2.17时，甲烷能、单位干物质采食量的甲烷产量、单位总能摄入量的甲烷能产量显著降低（P<0.05）。结果提示，在平均日增重一致的前提下，高NFC/NDF饲粮料重比较低，且甲烷转化效率较低；对生长期杜寒杂交母羊来说，限饲条件下饲喂NFC/NDF为2.17的饲粮在提高生产效益的同时又兼顾甲烷减排，效果相对最佳。
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