Subjects: Materials Science >> Materials Science (General) submitted time 2023-03-31 Cooperative journals: 《腐蚀科学与防护技术》
Abstract： none
Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2022-12-01 Cooperative journals: 《中国全科医学》
Abstract：
Background The Gastrointestinal Symptom Rating Scale (GSRS) is widely used in domestic research. However, the Mandarin translation of the current Chinese version of the scale is not standardized. In addition, the measurement performance test of the GSRS has not been reported in any literature. Objective To evaluate the reliability, validity and responsiveness of the Chinese version of the Gastrointestinal Symptom Rating Scale (GSRS) and to provide an objective basis for its clinical and scientific application. Methods Patients who accessed gastrointestinal departments in 45 hospitals across China from November 2021 to March 2022 were chosen in a random order using the convenience sampling method. Their gastrointestinal symptoms were evaluated with the Chinese version of GSRS. The Chinese version of GSRS was used for reliability and validity analyses, and the paired Wilcoxon rank sum test was applied to compare changes in patients' scale scores before and after treatment. The effect size (ES), standardized response mean (SRM) and score change rate (CR) were used to compare the responsiveness of GSRS among patient populations with different diseases. Results For reliability analysis, the Cronbach's alpha, Guttman Split-half coefficient and the Spearman-Brown coefficient of the Chinese version of the GSRS were 0.896, 0.920 and 0.926, respectively. The two retest result’s intraclass correlation coefficient (ICC) was 0.589, and the Spearman correlation coefficient was 0.662. The content validity index (CVI) for each item (I-CVI) ranged from 0.78 to 1.00 for the validity analysis, the CVI for scale level universal agreement was 1, and the average CVI (S-CVI/Ave) was 0.96. The results of the exploratory factor analysis indicated that with a cumulative variance contribution rate of 60.721%, three common factors were extracted, each of which had an eigenvalue larger than 1. The confirmatory factor analysis results demonstrated that the data samples did not fit the initial model M0 desirably created by EFA. However, after correction by the modification indicator hints, all of the obtained relevant indicators for the new model M1 were within the acceptable range. Before and two weeks after therapy, there was a significant difference in the GSRS score (P <0.001). The ES and SRM of the GSRS were, respectively, 1.03 and 1.01. The CR of the GSRS scores was 74.32%. Conclusion The Chinese version of the GSRS is well suited for measuring the general population who present with gastrointestinal symptoms and evaluating treatment efficiency because of its high degree of measurement properties, including good reliability, validity, and responsiveness.
Subjects: Biology >> Bioengineering submitted time 2018-12-06 Cooperative journals: 《中国生物工程杂志》
Abstract：T淋巴细胞受体（TCRs）在抗原识别免疫应答中作用重要，其多样性与宿主免疫反应及肿瘤预后判断密切相关。目的：采用高通量测序技术研究前列腺癌组织和癌旁组织中T细胞受体（TCR）克隆多样性及其克隆序列。方法：收集5例PC患者的癌组织和癌旁组织，提取DNA后经多重PCR技术对TCR β链CDR3区进行扩增。用Illumina MiSeq进行测序，经过数据处理和比对分析，比较前列腺癌组织TCR CDR3组库的组成特征。结果：前列腺癌组织具有更高程度的克隆百分比和较高的高度扩增克隆比HEC（HEC：频率>样本总读数的0.5%的TCR克隆），并获得了24对在癌组织样本中差异表达的V-J基因组合、16对在癌旁组织样本中差异表达的V-J基因组合。结论：前列腺癌组织与癌旁组织均存在差异性的V-J基因组合以及高克隆表达的HEC，其中癌组织样品具有更高的HEC。本研究对PC发生发展的免疫学研究提供了新的数据，对PC免疫监视、T细胞受体变异标志等研究提供了参考，对以后继续研究打下了基础。
Subjects: Biology >> Zoology submitted time 2017-10-11 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究饲粮添加氯化胆碱对19~42周龄蛋鸡生产性能和蛋品质的影响。选取990只18周龄京红1号蛋鸡，随机分为6组，每组12个重复，每个重复15只鸡，分别饲喂在基础饲粮中添加0、1 000、2 000、4 000、8 000和16 000 mg/kg氯化胆碱的试验饲粮。试验期24周。结果显示：1）19~42周龄，2 000、4 000、8 000和16 000 mg/kg组产蛋率显著低于0 mg/kg组（P0.05）。2）2 000 mg/kg组20、22周龄的蛋壳厚度显著高于0 mg/kg组（P<0.05），16 000 mg/kg组22、38周龄蛋壳强度显著低于0 mg/kg组（P<0.05）。16 000 mg/kg组26周龄蛋白高度和哈夫单位显著低于0 mg/kg组（P<0.05），8 000 mg/kg组22周龄哈夫单位显著高于0 mg/kg组（P<0.05）。20、21、22和38周龄，1 000、2 000、4 000、8 000和16 000 mg/kg组蛋黄颜色显著高于0 mg/kg组（P<0.05）。3）1 000、2 000、4 000、8 000和16 000 mg/kg组24周龄全蛋磷脂酰胆碱含量显著高于0 mg/kg组（P<0.05），16 000 mg/kg组27、38周龄全蛋磷脂酰胆碱含量显著高于0 mg/kg组（P<0.05）。16 000 mg/kg组38周龄蛋黄磷脂酰胆碱含量显著高于0 mg/kg组（P<0.05）。由此可见，饲粮添加适量氯化胆碱能提高蛋壳厚度、蛋壳强度、蛋黄颜色，适宜添加量为1 000 mg/kg。而饲粮添加高于1 000 mg/kg氯化胆碱能降低产蛋率；添加高于4 000 mg/kg氯化胆碱能降低蛋重和采食量。
Hits
Hits
Downloads
Comment