Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-27 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the antitumor effect of lycorine on renal cell carcinoma ACHN cells and explore the possible mechanism. Methods We used flow cytometry to examine the effect of lycorine on ACHN cell cycle and apoptosis. The cell proliferation, migration and invasion were assessed with MTS assay, wound healing assay, and Transwell assay, respectively. Colony forming assay was performed, and the mRNA and protein levels of Bax, Bcl-2, survivin, caspase-3, cyclin D1 and CDK4 were measured with qRT-PCR and Western blotting. Results Lycorine obviously inhibited the proliferation of ACHN cells with an IC50 of 24.34 μmol/L. Lycorine also induced apoptosis of ACHN cells, caused cell cycle arrest at G0/G1 phase, and suppressed the colony forming ability of the cells in a dose-dependent manner. The migration and invasion of ACHN cells were significantly inhibited by 5 μmol/L lycorine. Lycorine up-regulated the mRNA levels of CDK4, Bax, caspase-3 while down-regulated the levels of survivin, Bcl-2 and Cyclin D1; the protein levels of CDK4 and Bax were increased and cyclin D1, Bcl-2 and surviving expressions were decreased, but caspase-3 expression showed no significant changes following the treatment. Conclusion Lycorine has obvious antitumor effect against ACHN cells, suggesting its value as a new therapeutic agent for renal cell carcinoma.
Subjects: Biology >> Zoology submitted time 2017-10-10 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究二肽螯合铁对仔猪生长性能、血清铁和抗氧化指标的影响。选取40日龄健康三元杂交仔猪80头，随机分为对照组和试验组2个组，每组4个重复，每个重复10头猪。对照组和试验组饲粮中的铁添加量均为180 mg/kg（以铁计），对照组仅添加硫酸亚铁，试验组仅添加二肽螯合铁。试验期21 d。结果表明，与对照组相比，试验组平均日增重(ADG)提高了10.37%(P＞0.05)，料重比(F/G)降低了5.59%(P<0.05)；试验组血清铁含量显著高于对照组(P<0.05)，血清铁蛋白和血红蛋白含量高于对照组(P＞0.05)；试验组血清超氧化物歧化酶(SOD)活性显著高于对照组(P<0.05)，血清丙二醛（MDA）含量显著低于对照组(P<0.05)。由此可见，二肽螯合铁可明显促进仔猪生长，改善仔猪体内铁代谢状况，增强仔猪抗氧化能力。
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