Predictive Value of Tumor Budding and Tumor-infiltrating Lymphocytes on Lymph Node Metastasis of Esophageal Squamous Cell Carcinoma

Subjects: Medicine, Pharmacy >> Preventive Medicine and Hygienics submitted time 2023-05-11 Cooperative journals: 《中国全科医学》

ZHANG Jifang CHEN Fang TANG Jiawen LI Hongliang

Abstract： Background  The surgical protocols and prognosis of esophageal squamous cell carcinoma（ESCC） patients influenced by the status of lymph node metastasis. It is necessary to identify risk factors that can predict lymph node metastasis to assist in clinical treatment. Objective  To investigate the predict value of tumor budding（TB）and tumor-infiltrating lymphocytes（TILs）on lymph node metastasis（LNM）in patients with ESCC. Methods  A total of 124 patients with ESCC who underwent radical resection and retained paraffin-embedded specimens in the Affiliated People's Hospital of Ningbo University from January 2013 to March 2022 were selected as the research subjects. The light microscopy and CK5/6 immunohistochemical staining were used to evaluate the amount of TB and density of TILs. Multivariate Logistic regression analysis was used to explore the influencing factors of lymph node metastasis in patients with ESCC and the receiver operating characteristic（ROC）curve was plotted to investigate the predictive value of TB and TILs for lymph node metastasis in patients with ESCC. Results  Multivariate Logistic regression analysis showed that TB（OR=20.078，P<0.001），TILs（OR=0.218，P=0.008），and intravascular tumor thrombus（OR=28.609，P<0.001）were influencing factors for lymph node metastasis. The area under ROC curve（AUC）for TB and TILs to predict lymph node metastasis in patients with ESCC was 0.835〔95%CI（0.763，0.907）〕and 0.656〔95%CI（0.558，0.753）〕，respectively. Conclusions  High-grade TB and low-density TILs were influencing factors for lymph node metastasis，TB grade and density of TILs has good diagnostic efficacy in predicting lymph node metastasis in patients with ESCC.

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