分类: 医学、药学 >> 临床医学 提交时间: 2023-11-10 合作期刊: 《中国全科医学》
摘要: none
分类: 核科学技术 >> 核探测技术与核电子学 提交时间: 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要: In advanced technologies, single event upset reversal (SEUR) due to charge sharing can make the upset state of SRAM cells recover to their initial state, which can reduce the soft error for SRAMs in radiation environments. By using the full 3D TCAD simulations, this paper presents a new kind of SEUR triggered by the charge collection of the Off-PMOS and the delayed charge collection of the On-NMOS in commercial 40-nm 6 T SRAM cells. The simulation results show that the proposed SEUR can not occur at normal incidence, but can present easily at angular incidence. It is also found that the width of SET induced by this SEUR remains the same after linear energy transfer (LET) increases to a certain value. In addition, through analyzing the effect of the spacing, the adjacent transistors, the drain area, and some other dependent parameters on this new kind of SEUR, some methods are proposed to strengthen the recovery ability of SRAM cells.
分类: 生物学 >> 生物物理学 提交时间: 2016-05-11
摘要: Adaptations to hypoxia play an important role in Mycobacterium tuberculosis pathogenesis. Rv0324, which contains an HTH DNA-binding domain and a rhodanese domain, is one of the key transcription regulators in response to hypoxia. M. tuberculosis Rv1674c is a homologue of Rv0324. To understand the interdomain interaction and regulation of the HTH domain and the rhodanese domain, recombinant Rv1674c protein was purified and crystallized by the vapour-diffusion method. The crystals diffracted to 2.25 angstrom resolution. Preliminary diffraction analysis suggests that the crystals belonged to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 67.8, c = 174.5 angstrom, alpha = beta = 90, gamma = 120 degrees. The Matthews coefficient was calculated to be 2.44 angstrom(3) Da(-1), assuming that the crystallographic asymmetric unit contains two protein molecules.
分类: 核科学技术 >> 裂变堆工程技术 提交时间: 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要: Oxygen control technology is a critical issue for compatibility of candidate structural materials with liquid lead-bismuth eutectic (LBE) in accelerator driven systems. Performances of a self-developed Pt/air sensor and another one from Karlsruher Institute of Technology (KIT) were tested in stagnant oxygen-saturated liquid LBE. Calibrations showed that the trend and values of corrected electromotive force (EMF) of the self-developed sensor, with a bias voltage of 20 mV, were consistent with theoretical results above 425 ℃, and similar results were obtained in cross-calibration test with EMF value of KIT sensor as reference. In stability test at 450 ℃ for 100 hours, the KIT sensor performed better than the self-developed one, which showed signal fluctuations. Both sensors exhibited quick response to temperature variations in the responsiveness test.
分类: 生物学 >> 生物物理学 提交时间: 2016-05-12
摘要: Tetrapyrroles, including haem and chlorophyll, play vital roles for various biological processes, such as respiration and photosynthesis, and their biosynthesis is critical for virtually all organisms. In photosynthetic organisms, magnesium chelatase (MgCh) catalyses insertion of magnesium into the centre of protoporphyrin IX, the branch-point precursor for both haem and chlorophyll, leading tetrapyrrole biosynthesis into the magnesium branch1,2. This reaction needs a cooperated action of the three subunits of MgCh: the catalytic subunit ChlH and two AAA(+) subunits, ChlI and ChlD ( refs 3-5). To date, the mechanism of MgCh awaits further elucidation due to a lack of high-resolution structures, especially for the similar to 150 kDa catalytic subunit. Here we report the crystal structure of ChlH from the photosynthetic cyanobacterium Synechocystis PCC 6803, solved at 2.5 angstrom resolution. The active site is buried deeply inside the protein interior, and the surrounding residues are conserved throughout evolution. This structure helps to explain the loss of function reported for the cch and gun5 mutations of the ChlH subunit, and to provide the molecular basis of substrate channelling during the magnesium-chelating process. The structure advances our understanding of the holoenzyme of MgCh, a metal chelating enzyme other than ferrochelatase.