Research on the Mechanism of Action of Simiao Yongan Decoction in the Treatment of Diabetic Foot Based on Metabolomics postprint

Abstract: Background　In recent years，diabetic foot has become an important cause of disability and death among diabetic patients. With the advancement of the times and technology，modern medicine and traditional Chinese medicine have gradually deepened their understanding and research on diabetic foot，further improving the treatment methods for diabetic foot. To a certain extent，this can relieve the pain of patients and improve their quality of life. Objective　The mechanism of action of Simiao Yongan Decoction in the treatment of diabetic foot was explored by combining metabolomics with network pharmacology. Methods　Diabetic foot patients who were hospitalized in the Burn Department，Vascular Surgery Department of Heilongjiang Provincial Hospital and the Vascular Surgery Department of Nangang Campus from July 2023 to August 2024 were selected as the research subjects. A total of 60 patients with heat-toxin intense diabetic foot who met the criteria were included in this study. They were randomly divided into debridement group，Western medicine group，traditional Chinese medicine group and combined Chinese and Western medicine group，with 15 cases in each group. Another 15 normal people were included as the control group.Firstly，databases and software such as TCMSP，GeneCards，OMIM，and TTD were used to search for the pharmacodynamic basis，target sites and metabolic pathways of Simiao Yongan Decoction on diabetic foot. The serum metabolomics and network pharmacology of Simiao Yongan Decoction were integrated for research to verify the possible active ingredients and metabolic pathways of Simiao Yongan Decoction in the intervention of diabetic foot. Results　The main active ingredients of Simiao Yongan Decoction for treating diabetic foot are luteolin，quercetin and acanthopanthin，etc. Diabetic foot may be treated by regulating pathways such as mitogen-activated protein kinase signaling pathway，tumor necrosis factor signaling pathway，phosphatidyacyl alcohol signaling pathway，HIF-1 signaling pathway，and Toll-like receptor signaling pathway through targets such as AKT1，TNF，HSP90AA1，MAPK8，and STAT3. Among them，the phospholipacyl alcohol signaling pathway is consistent with the research results of the serum metabolomics of Simiao Yongan Decoction. Conclusion　The phosphatidylinositol signaling pathway may be the one through which Simiao Yongan Decoction interferes with the metabolism of diabetic foot.