瑶药红牛膝质量控制方法研究 postprint

Abstract: Yao medicine Hongniuxi is the root and rhizome of Achyranthes longifolia, which was a commonly used medicinal herb in Yao ethnic areas. Currently, only a few provinces have recorded the method for quality control of A. longifolia. Microscopic method and thin layer chromatography were qualitatively identified on the roots and rhizomes of 14 batches of Yao Medicine A. longifolia collected in Guangxi region in the standy. The content of β-ecdysterone was determined by High Performance Liquid Chromatography. The Phenomenex column (250mm × 4.6 mm, 5 μm) was used, the mobile phase was acetonitrile-water (15:85) , the column temperature was 25 ℃, the flow rate was 1.0 mL‧min-1, and the detection wavelength was at 243 nm. The results were as follows: (1) The characteristics and microscopic identification methods of A. longifolia were obvious. Microscopic identification showed the inner layer of thrombus, vascular bundle, abnormal vascular bundle, duct, ray cell, calcium oxalate sand crystal or square crystal, etc. (2) The identification spots of β-ecdysterone and ginsenoside RO thin layer chromatography are clear, and the separation effect was good. (3) The β-ecdysterone showed a good linear relationship within the range of 5.606-224.224 μg (r＝0.999 9), whose average recovery (n=6) was 97.68%, with the RSD of 1.37%. (4) Among 14 batches of samples, the content of β-ecdysterone range from 0.005% to 0.158%, with an average content of 0.098%. This study established a complete method for microscopic identification, thin-layer identification, and content determination, providing a scientific basis for rational evaluation of the quality of A. longifoli. and clinical rational drug use.