摘要: Advances in RNA research and RNA nanotechnology depend on the ability to manipulate and probe RNA with high precision through chemical approaches, both invitro and in mammalian cells. However, covalent RNA labeling methods with scope and versatility comparable to those of current protein labeling strategies are underdeveloped. A method is reported for the site- and sequence-specific covalent labeling of RNAs in mammalian cells by using tRNA(Ile2)-agmatidine synthetase (Tias) and click chemistry. The crystal structure of Tias in complex with an azide-bearing agmatine analogue was solved to unravel the structural basis for Tias/substrate recognition. The unique RNA sequence specificity and plastic Tias/substrate recognition enable the site-specific transfer of azide/alkyne groups to an RNA molecule of interest invitro and in mammalian cells. Subsequent click chemistry reactions facilitate the versatile labeling, functionalization, and visualization of target RNA.
-
期刊:
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
-
分类:
生物学
>>
生物物理学
-
引用:
ChinaXiv:201605.01192
(或此版本
ChinaXiv:201605.01192V1)
DOI:10.12074/201605.01192V1
CSTR:32003.36.ChinaXiv.201605.01192.V1
- 推荐引用方式:
Li, Fahui,Dong, Jianshu,Gong, Weimin,Li, Jiasong,Wang, Jiangyun,Li, Fahui,Dong, Jianshu,Gong, Weimin,Li, Jiasong,Wang, Jiangyun,Wang, Jiangyun,Hu, Xiaosong,Shen, Jing,Tian, Huifang.A Covalent Approach for Site-Specific RNA Labeling in Mammalian Cells.中国科学院科技论文预发布平台.[ChinaXiv:201605.01192V1]
(点此复制)