Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-01-25 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the effect of propofol on myelin basic protein (MBP) expression in oligodendrocytes of SD rats at different developmental stages. Methods This study was conducted in 3-, 7-, 14- and 21-day-old SD rats (40 in each age group). In each group, the rats were randomized equally into control group and experimental group, and in the control group, the rats received an intraperitoneal injection of 25 mg/kg medium-long-chain fat emulsion followed by injections at a half dose every 20 min for 8 h; the rats in the experimental group were given injections of propofolmedium (at the initial dose of 25 mg/ kg) in the same manner. The transcriptional levels of MBP and caspase-3 in the brain tissues were detected by qRT-PCR, and the protein expression of MBP was with Western blotting and immunehistochemistry. Results Compared with those in the control groups, the expression of MBP mRNA was significantly down-regulated while caspase-3 mRNA was up-regulated in 3-, 7- and 14-day-old rats in the experimental groups (P<0.05). The protein expression of MBP in 7- and 14-day-old rats was significantly decreased in the experimental groups compared with the control groups (P<0.05). The expression of caspase-3 mRNA or MBP protein in 21-day-old rats showed no significant difference between the two groups (P>0.05). Conclusion Propofolcandown-regulatetheexpressionofMBPatboththemRNAandproteinlevelsinSDrats,especiallyinthoseat7and 14daysofage.
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-21 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the effects of health Qigong Wuqinxi exercise on mild depression in college students and analyze the changes in hydrogen proton magnetic resonance spectra (1H-MRS) in the prefrontal cortex and hippocampus after the exercise. Methods Fifty-eight volunteer college students, including 30 with mild depression and 28 healthy students, were randomized into the intervention group and non-intervention group. The students in the intervention group were asked to practice health Qigong Wuqinxi training for 12 weeks and those in the non-intervention group did not engage in such training.For each subject, BECK Depression Self-reported questionnaire (BDI), Hamilton Depression rating scale (HAMD) score, and the metabolic parameters of 1H-MRS in the prefrontal cortex and hippocampus were evaluated before and after the intervention. Results Before the intervention, the scores of BDI and HAMD in the depression group were significantly higher than that in the control group (P<0.01), and were lowered obviously after the 12-week intervention (P<0.01). Compared with the control group, 1H-MRS in the depression group before intervention showed significantly increased NAA/Cr value in the left prefrontal cortex, Cho/Cr value in the bilateral hippocampus and the left frontal lobe, and Cho/Cr value of the left hippocampus and right frontal lobe (P<0.05) with significantly lowered NAA/Cho value in the bilateral prefrontal and Cho/ NAA value in the right hippocampus (P<0.05). After 12 weeks of intervention, NAA/Cr value in the bilateral hippocampus and the NAA/Cho value in the right hippocampus were significantly lowered (P<0.05), and NAA/Cho value in the right prefrontal and Cho/NAA value in the right hippocampus were significantly increased (P<0.05) in the depression group. Before the intervention, Pearson correlation analysis showed that the scores of HAMD and BDI were positively correlated with Cho/Cr value in the hippocampus and NAA/Cr value in prefrontal lobe (P<0.01) and inversely with NAA/Cho in prefrontal lobe and Cho/NAA value in the hippocampus (P<0.05); after the intervention, the scores of HAMD and BDI were positively correlated with NAA/Cr value in the hippocampus and Cho/Cr value in the left hippocampus (P<0.05). Conclusion Exercise of health Qigong Wuqinxi can reduce depression scale scores in patients with mild depression and improve the metabolic indexes (NAA/Cr and Cho/Cr values) in the prefrontal cortex and the hippocampus.
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