Subjects: Biology >> Botany submitted time 2023-01-30 Cooperative journals: 《广西植物》
Abstract：
In order to find culturable bacteria resources and antibacterial activity strain in the rhizosphere soil of mangrove plants from the Maowei Sea, the rhizosphere soil of five mangrove plants(Rhizophora stylosa, Talipariti tiliaceum, Sonneratia apetala, Aegiceras corniculatum, and Pluchea indica) was cultured by enrichment media and then isolated the culturable bacteria. The culturable bacteria were purified by six different isolation media. The diversity of culturable bacteria was analyzed by the 16S rRNA gene sequence information. Antimicrobial activities (Staphylococcus epidermidis, Methicillin-resistant Staphylococcus aureus, and Pseudomonas aeruginosa) of the culturable bacteria were analyzed by disk diffusion method. The results were as follows: (1) A total of 120 strains were isolated from 7 rhizosphere soil samples of mangrove plants which could be classified into 35 families and 47 genera, among which 5 potential new species were obtained. The dominat genus were Streptomyces (14.2%). (2) It was found that the crude extracts of 9 strains had antibacterial activity against at least one pathogen. The results indicate that there are abundant culturable bacteria and potential new antibiotics resources in the mangrove rhizosphere soils from the Maowei Sea.
Subjects: Biology >> Botany submitted time 2022-12-01 Cooperative journals: 《广西植物》
Abstract：
The hypocotyl of Bruguiera gymnorhiza is a common marine herbal medicine of the Jing people used in the treatment of hepatitis B. Firstly, MTT and Real-time PCR methods were used to determine the anti-HBV ability of different extracted parts of the hypocotyl of B. gymnorhiza, and then the chemical constituents of the active extraction parts were separated and identified using modern chromatographic techniques and spectroscopic methods. Finally, an in vitro screening model was employed to test the anti-HBV activity of obtained compounds. The results were as follows: The n-butanol phase of the hypocotyl exhibited anti-HBV activity; 11 compounds, namely uridine (1), thymidine (2), adenosine (3), oryzalactam (4), n-butyl-O-D-fructopyranoside (5), nortetillapyrone (6), (4R,6S)-4-meth-oxyl-2,3-dihydroaquilegiolide (7), (4R,6S)-2-dihydromenisdaurilide (8), gallcatechin (9), 1-(4-hydroxy-3-meth-oxy)-phen yl-2-[4-(1,2,3-trih-ydroxypropyl)-2-methoxy]-phenoxy-1,3-propandiol(10), and (‒)-lyoniresinol- 9-O-β-D-xylopyrano-side (11), were isolated and structurally identified, among them, compounds 4-5 and 7-8 were firstly obtained from B. gymnorhiza; Compound 4 showed anti-HBV activity with an inhibition rate of 23.59%. The study clarify the chemical composition of the anti-HBV of B. gymnorhiza hypocotyl.
Subjects: Biology >> Botany submitted time 2022-12-01 Cooperative journals: 《广西植物》
Abstract：
In order to explore the antibacterial activity of endophytic fungi derived from Aegiceras corniculatum, antibacterial activity of ferment extract of endophytic fungi was used as evaluation index to screen active strains, the chemical constituents were separated on the basis of bioactivity-guiding method combined with various chromatographic techniques and identified by spectral technique together with comparison with literature data, and the antibacterial activity of pure compounds was determined by microplate method. The results were as follows: (1) Sixteen taxa of endophytic fungi isolated from A. corniculatum were distributed in 2 classes, 7 orders, 10 families and 10 genera. Fusarium was dominant genus. The ferment extract of endophytic fungi GXIMD02029 and GXIMD02039 displayed different levels of inhibitory effects on Bacillus subtilis, Staphylococcus epidermidis, methicillin-resistant S. aureus, Micrococcus luteus, Actinomyces viscosity, and Staphylococcus aureus when that of GXIMD02038 against methicillin-resistant Staphylococcus aureus, Micrococcus luteus, and Staphylococcus aureus. (2) Seven pure compounds were isolated from endophytic fungi Phomopsis sp. GXIMD02029 and identified as (15R)-acetoxydothiorelone A (1), cytosporone B (2), pestalotiopsone H (3), pestalotiopsone B (4), 4-Hydroxybenzaldehyde (5), p-Hydroxybenzoic acid (6), N-(2-phenylethyl)acetamide (7). (3) Compounds 1 and 2 showed different levels of antibacterial effects. Compound 1 exhibited inhibitory effect on Bacillus subtilis, Staphylococcus epidermidis and methicillin-resistant S. aureus with MIC values of 16.25 g·mL-1, Micrococcus luteus and Actinomyces viscosity with MIC values of 7.812 5 g·mL-1, and Staphylococcus aureus with MIC value of 31.25 g·mL-1. Compound 2 displayed inhibitory effect on Micrococcus luteus with MIC value of 62.5 g·mL-1, Bacillus subtilis, Staphylococcus epidermidis, methicillin-resistant S. aureus and Actinomyces viscosity with MIC values of 125 g·mL-1, and Staphylococcus aureus with MIC value of 250 g·mL-1. Three active strains were reported in the project. It is reported that Compound 1 showed the antibacterial activity for the first time. The project provides the basis for the antibacterial values of endophytic fungi of Aegiceras corniculatum.
Subjects: Biology >> Botany >> Applied botany submitted time 2022-03-30 Cooperative journals: 《广西植物》
Abstract： Marine plants and their associated microorganisms can produce many secondary metabolites with novel structures and unique activities. The Beibu Gulf is rich in marine plant species resources. According to statistics, there are 43 species of marine plants in 3 phyla. This paper reviews the research progress of the secondary metabolites of marine plants and their co-epiphytic microorganisms in the Beibu Gulf since 2002. 59 new compounds and 35 known active compounds were obtained from 11 species of mangrove plants and 7 species of associated microorganisms, and 3 new compound and 7 known active compounds were obtained from 3 species of seagrass plants. 25 new compounds and 8 known active compounds were obtained from 6 species of algae plants and 1 species of symphytic microorganisms, which mainly involved terpenoids, alkaloids, flavonoids and sterols, most of which had good anti-bacterial, anti-oxidation, anti-tumor, anti-inflammatory and enhancing the body immunity. Further research suggestions are put forward. The review provide reference for further study and utilization of marine plants and their associated microorganisms in Beibu Gulf.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-11-04 Cooperative journals: 《广西植物》
Abstract： Mangrove habitat is rich in microfloras and its secondary metabolites have novel structure, which is an important source for mining new drugs. This study preliminarily evaluated the culturable bacterial diversity of sediments, roots, leaves and hypocotyl of Bruguiera gymnorrhiza and the biological activity of bacterial metabolites, and looked for anti-HBV drug source strains. Pure culture technique and 16S rRNA molecular biology technique were employed to determine the species of bacteria and analyze the species diversity. Using HepG2.2.15 cell line as a model, the anti-HBV activity of bacterial metabolites was tested by MTT and PCR techniques. The secondary metabolites of active bacteria were preliminary analyzed by LC-HRMS. The results were as follows: (1) A total of 59 species of bacteria were obtained, which belonging to 4 phyla, 5 classes, 14 orders, 23 families, and 36 genera, among which Bacillus was the dominant genus. Strains GXIMD07402, GXIMD07665 and GXIMD07384 are potential new species of Pseudooceanicola, Thioclava, and Aestuariibaculum, respectively. (2) The results of anti-HBV activity showed that GXIMD07366, GXIMD07616, GXIMD07384, GXIMD07550 and GXIMD07445X could significantly reduce the level of HBV DNA in the supernatant of HepG2.2.15 cells, and the inhibition rates were 51%, 47%, 63%, 52%, and 47%. (3) Four main secondary metabolites of the highly active strain GXIMD07384 were identified as Adenosine, Cyclo(L-Pro-L-OMet), Acremine G, and 7,8-dimethylbenzo[g]pteridine -2,4(1H,3H)-dione. The results of the study confirm that the species diversity of culturable bacteria in the habitat of Bruguiera gymnorrhiza is rich and contains anti-HBV active strains, which provide a basis for the subsequent application of marine microbial resources.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-06-02 Cooperative journals: 《广西植物》
Abstract： This study aims to explore potential new bacterial species and bioactive substances in mangrove plants, which can enrich the diversity of mangrove microorganisms and provide strain resources for the development of new active products. 22 samples were collected from three true mangrove plants of Kandelia candel, Bruguiera gymnorrhiza, Rhizophora stylosa, and their habitats, in Guangxi coastal area. Then 22 samples were divided according to different parts such as roots, stems, leaves, flowers, fruits and muds. Eight different culture mediums were used to isolate culturable bacteria, and 16S rRNA gene sequences were used to identify bacteria and analyze diversity. For cultured bacteria, the antibacterial and enzyme activities of fermented crude extracts were screened with Kirby-Bauer method and spot planting method, respectively. The results were as follows:（1）Based on sequencing results of 16S rRNA gene, a total of 35 culturable bacteria were isolated, belonging to 28 genera and 23 families. Bacillus accounted for 14.3% of the total bacteria, which was the dominant bacteria. Meanwhile, 11 potential new bacterial species were found. （2）Four strains of bacteria showed antibacterial activities, 16 strains had enzyme activities, and Bacillus was the dominant strain in enzyme activities. The above results showed that Guangxi true mangrove plants were rich in bacterial diversity, and some bacteria showed antibacterial activities and enzyme activities. The new bacterial species and active strain had certain development potential in the new antibiotics and enzymes application.
Subjects: Biology >> Botany >> Applied botany submitted time 2019-03-14 Cooperative journals: 《广西植物》
Abstract：设计9种分离培养基，采用稀释涂布法从14份真红树植物的46份组织样品中分离纯化内生细菌。基于菌株形态学特征和16S rRNA基因序列确定分离菌株的种属，并分析其物种多样性，采用秀丽隐杆线虫模型筛选菌株延缓衰老活性。结果表明：通过基因序列去重复后从46份真红树植物组织样品中获得32株海洋细菌，基于菌株16S rRNA基因序列信息分析，覆盖12科17属，其中芽孢杆菌属（Bacillus）为优势菌属，并获得1株疑似橙单胞菌属（Aurantimonas）新种，16S rRNA基因序列相似性低于97%。经过秀丽隐杆线虫粗筛发现3株海洋细菌具有显著延缓线虫衰老的活性（P<0.05）。研究结果表明海南西海岸真红树内生细菌具有物种多样性，部分菌株具有延缓线虫衰老活性。
Subjects: Biology >> Botany >> Applied botany submitted time 2018-10-26 Cooperative journals: 《广西植物》
Abstract：无瓣海桑果实为真红树无瓣海桑的果实。研究无瓣海桑果实不同提取物对 D-半乳糖所致衰老小鼠学习记忆能力影响及其作用机制。采用 Morris 水迷宫实验测量无瓣海桑果实不同提取物对小鼠的学习记忆能力影响，HE 染色观察各组小鼠脑部神经细胞的变化情况，并测定脑组织超氧化物歧化酶（SOD）活力、谷胱甘肽过氧化物酶（GSH-Px）活力、一氧化氮（NO）含量和单胺氧化酶（MAO）活力。结果表明与模型组相比，无瓣海桑果实不同提取物处理组小鼠在水迷宫实验中逃避潜伏期明显缩短（P＜0.05），目标象限停留时间明显增加（P＜0.05）。无瓣海桑果实不同提取物处理组小鼠脑部神经细胞损伤与模型组相比明显减少，小鼠脑部 SOD 酶活力和 GSH-Px 酶活力提高（P＜0.05），NO 含量和 MAO 活力在脑部显著降低（P＜0.05）。无瓣海桑果实不同提取物对 D-半乳糖致衰老小鼠学习记忆能力有改善作用，无瓣海桑果实不同提取物通过提高小鼠脑内源抗氧化酶（SOD、GSH-Px）活力，降低脑部 NO 含量和 MAO 活力来提高 D-半乳糖致衰老小鼠的学习记忆能力。
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