Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-27 Cooperative journals: 《南方医科大学学报》
Abstract: Objective To construct a MYH9 gene knockout model in MGC803 cell line using transcription activator-like effector nuclease (TALEN) and observe its effect on cell cycle and apoptosis. Methods According to FastTALE TM TALEN Kit, we designed TALEN pairs and constructed the plasmids targeting to MYH9 gene. After detecting their activity in MGC803 cells by plasmid transfection, DNA sequencing, RT-PCR and western blot, we selected the monoclonal cells and studied the changes in the cell cycle and apoptosis. Results MYH9 gene could not be knocked out but knocked down in selected MGC803 monoclonal cells, which caused cell cycle arrested at G2/M phase (P<0. 05) and a significant increase in the cell number with early apoptosis (P<0. 01). Conclusion We successfully generated a MYH9 knockdown model in MGC803 cell lines by TALEN, which could be in favor of MYH9 function study in gastric cancer.
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-07 Cooperative journals: 《分子影像学杂志》
Abstract: The assessment of tumor invasive depth, metastasis and if surgical margin had residual cancer were in urgent need of an in situ, real-time diagnosis technology to determine surgical treatment of gastrointestinal tract malignant tumor and theextent of resection surgery. Multiphoton microscopy can provide gastrointestinal tissue structure and cell morphology of real-time information. Multiphoton imaging technology had no exogenous marker of tissue, was extremely sensitive to collagen, had light of the tissue damage and deep penetration depth. Such characters enable the technology to be applied ingastrointestinal tumor as a new optical biopsy. The review aimed to overview the feasibility of multiphoton imaging forevaluation of gastrointestinal tumor optical biopsy and had a discussion on the development prospect of multiphoton imaging technology considerable.