分类： 生物学 >> 植物学 提交时间： 2017-07-18
摘要： Studies of the anther transcriptome on non-model plants without a known genome are surprisingly scarce. RNA-Seq and digital gene expression (DGE) profiling provides a comprehensive approach to identify candidate genes contributing to developmental processes in non-model species. Here we built a transcriptome library of developing anthers of Hamelia patens and analysed DGE profiles from each stage to identify genes that regulate tapetum and pollen development. In total 7,720 putative differentially expressed genes across four anther stages were identified. The number of putative stage-specific genes was: 776 at microspore mother cell stage (MMC), 807 at tetrad stage (TET), 322 at uninucleate microspore stage (UNM) and the highest number (1,864) at bicellular pollen stage (BCP). GO enrichment analysis revealed 243 differentially expressed and 108 stage-specific genes that are potentially related to tapetum development, sporopollenin synthesis and pollen wall. The number of expressed genes, their function and expression profiles were all significantly correlated with anther developmental processes. Overall comparisons of anther and pollen transcriptomes with those of rice and Arabidopsis together with the expression profiles of homologs of known anther-expressed genes, revealed conserved patterns and also divergence. The divergence may reflect taxon-specific differences in gene expression, the use RNA-seq as a more sensitive methodology, variation in tissue composition and sampling strategies. Given the lack of genomic sequence, this study succeeded in assigning putative identity to a significant proportion of anther-expressed genes and genes relevant to tapetum and pollen development in H. patens. The anther transcriptome revealed a molecular distinction between developmental stages, serving as a resource to unravel the functions of genes involved in anther development in H. patens and informing the analysis of other members of the Rubiaceae.
分类： 物理学 >> 核物理学 提交时间： 2016-09-14
Xiaoyuan Huang
Anna S. Lamperstorfer
Yue-Lin Sming Tsai
Ming Xu
Qiang Yuan Jin Chang
Yong-Wei Dong
Bing-Liang Hu
Jun-Guang L╱
Le Wang
Bo-Bing Wu
Shuang-Nan Zhang
摘要： HERD is the High Energy cosmic-Radiation Detection instrument proposed to operate onboard China's space station in the 2020s. It is designed to detect energetic cosmic ray nuclei, leptons and photons with a high energy resolution (∼1% for electrons and photons and 20% for nuclei) and a large geometry factor (>3m2sr for electrons and diffuse photons and >2m2sr for nuclei). In this work we discuss the capability of HERD to detect monochromatic γ-ray lines, based on simulations of the detector performance. It is shown that HERD will be one of the most sensitive instruments for monochromatic γ-ray searches at energies between ∼10 to a few hundred GeV. Above hundreds of GeV, Cherenkov telescopes will be more sensitive due to their large effective area. As a specific example, we show that a good portion of the parameter space of a supersymmetric dark matter model can be probed with HERD.
点击量
待评议
点击量
下载量
评论
