• Spectroscopic localization of atomic sample plane for precise digital holography

    分类: 光学 >> 量子光学 提交时间: 2023-02-19

    摘要: In digital holography, the coherent scattered light fields can be reconstructed volumetrically. By refocusing the fields to the sample planes, absorption and phase-shift profiles of sparsely distributed samples can be simultaneously inferred in 3D. This holographic advantage is highly useful for spectroscopic imaging of cold atomic samples. However, unlike (e.g., biological samples or solid particles), the quasi-thermal atomic gases under laser-cooling are typically featureless without sharp boundaries, invalidating a class of standard numerical refocusing methods. Here, we extend the refocusing protocol based on the Gouy phase anomaly for small phase objects to free atomic samples. With a prior knowledge on a coherent spectral phase angle relation for cold atoms that is robust against probe condition variations, an ``out-of-phase'' response of the atomic sample can be reliably identified, which flips the sign during the numeric back-propagation across the sample plane to serve as the refocus criterion. Experimentally, we determine the sample plane of a laser-cooled $^{39}$K gas released from a microscopic dipole trap, with a $\delta z\approx 1~{\rm \mu m}$$\ll 2\lambda_p/{\rm NA}^2$ axial resolution, with a NA=0.3 holographic microscope at $\lambda_p=770~$nm probe wavelength.

  • Metabolic engineering of proanthocyanidin production by repressing the isoflavone pathways and redirecting anthocyanidin precursor flux in legume

    分类: 生物学 >> 植物学 >> 植物生物化学、植物生物物理学 提交时间: 2016-05-04

    摘要: MtPAR is a proanthocyanidin (PA) biosynthesis regulator; the mechanism underlying its promotion of PA biosynthesis is not fully understood. Here, we showed that MtPAR promotes PA production by a direct repression of biosynthesis of isoflavones, the major flavonoids in legume, and by redirecting immediate precursors, such as anthocyanidins, flux into PA pathway. Ectopic expression of MtPAR repressed isoflavonoid production by directly binding and suppressing isoflavone biosynthetic genes such as isoflavone synthase (IFS). Meanwhile, MtPAR up-regulated PA-specific genes and decreased the anthocyanin levels without altering the expression of anthocyanin biosynthetic genes. MtPAR may shift the anthocyanidin precursor flux from anthocyanin pathway to PA biosynthesis. MtPAR complemented PA-deficient phenotype of Arabidopsis tt2 mutant seeds, demonstrating their similar action on PA production. We showed the direct interactions between MtPAR, MtTT8 and MtWD40-1 proteins from Medicago truncatula and Glycine max, to form a ternary complex to trans-activate PA-specific ANR gene. Finally, MtPAR expression in alfalfa (Medicago sativa) hairy roots and whole plants only promoted the production of small amount of PAs, which was significantly enhanced by co-expression of MtPAR and MtLAP1. Transcriptomic and metabolite profiling showed an additive effect between MtPAR and MtLAP1 on the production of PAs, supporting that efficient PA production requires more anthocyanidin precursors. This study provides new insights into the role and mechanism of MtPAR in partitioning precursors from isoflavone and anthocyanin pathways into PA pathways for a specific promotion of PA production. Based on this, a strategy by combining MtPAR and MtLAP1 co-expression to effectively improve metabolic engineering performance of PA production in legume forage was developed.

  • Imaging moving atoms by holographically reconstructing the dragged slow light

    分类: 光学 >> 量子光学 提交时间: 2023-02-19

    摘要: The propagation of light in moving media is dragged by atomic motion. The light-drag effect can be dramatically enhanced by reducing the group velocity with electro-magnetically induced transparency (EIT). We develop a systematic procedure to accurately reconstruct the complex wavefront of the slow light with single-shot measurements, enabling precise, photon shot-noise limited spectroscopic measurements of atomic response across EIT even in presence of generic atomic number fluctuations. Applying the technique to an expanding cloud of cold atoms, we demonstrate simultaneous inference of the atomic density distribution and the velocity field from the complex imaging data. This inline imaging technique may assist a wide range of cold atom experiments to access spectroscopic and phase space information with in situ and minimally destructive measurements.

  • The Chinese H{\alpha} Solar Explorer (CHASE) mission: An overview

    分类: 天文学 >> 天文学 提交时间: 2023-02-19

    摘要: The Chinese H{\alpha} Solar Explorer (CHASE), dubbed "Xihe" - Goddess of the Sun, was launched on October 14, 2021 as the first solar space mission of China National Space Administration (CNSA). The CHASE mission is designed to test a newly developed satellite platform and to acquire the spectroscopic observations in the H{\alpha} waveband. The H{\alpha} Imaging Spectrograph (HIS) is the scientific payload of the CHASE satellite. It consists of two observational modes: raster scanning mode and continuum imaging mode. The raster scanning mode obtains full-Sun or region-of-interest spectral images from 6559.7 to 6565.9 {\AA} and from 6567.8 to 6570.6 {\AA} with 0.024 {\AA} pixel spectral resolution and 1 minute temporal resolution. The continuum imaging mode obtains photospheric images in continuum around 6689 {\AA} with the full width at half maximum of 13.4 {\AA}. The CHASE mission will advance our understanding of the dynamics of solar activity in the photosphere and chromosphere. In this paper, we present an overview of the CHASE mission including the scientific objectives, HIS instrument overview, data calibration flow, and first results of on-orbit observations.