Subjects: Biology >> Botany >> Applied botany submitted time 2023-08-04 Cooperative journals: 《广西植物》
Abstract： To study the difference in the content of phenolics and the antioxidant capacity between the two kinds of Hawk tea of different maturity levels, and to evaluate their quality, this study used LC-MS/MS method to determine the content of 15 phenolic compounds. Then, the antioxidant activity of the two types of tea was evaluated using DPPH radical scavenging capacity, ABTS+ radical scavenging capacity, and Fe3+ reduction ability (FRAP). Then the content of the 15 phenolic compounds and the antioxidant efficacy between the two kinds of Hawk tea of different maturity levels were analyzed by analysis of variance (ANOVA). Based on the content of 15 phenolic compounds, hierarchical cluster analysis (HCA), principal component analysis (PCA), and orthogonal partial least square regression analysis (OPLS-DA) was used to classify the two kinds of Hawk tea. Finally, the contribution of different phenolic compounds in Hawk tea to antioxidation was discussed by partial least square regression analysis (PLSR). The results were as follows: (1) The contents of catechin, p-coumaric acid, isoquercitrin, hyperoside, nicotiflorin, astragalin, kaempferol, quercentin, and afzelin in tender-leaf tea were significantly higher than those in mature leaf tea, among which the average content of catechin, isoquercitrin, and astragalin were higher than thos in mature-leaf tea by 1 039, 169 and 257 mg�100 g-1, respectively. The results of HCA, PCA, and OPLS-DA can distinguish the two kinds of Hawk tea. (2) The ANOVA shows a significant difference in DPPH radical scavenging capacity, ABTS+ radical scavenging capacity, and Fe3+ reduction ability between the two kinds of tea, with the tender leaf superior to the mature leaf. (3) The PLSR suggests that isoquercitrin, catechin, astragalin, chlorogenic acid, hyperoside, p-coumaric acid, and kaempferol were the main phenolic chemical components that contributed to the antioxidant activity of Hawk tea. This study can provide a reference for Hawk tea's quality control and application promotion.
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