分类： 生物学 >> 微生物学 分类： 生物学 >> 植物学 提交时间： 2024-07-10
摘要： Plants evolved sophisticated genetic and metabolic mechanisms to shape their “core microbiome” under normal growth conditions. However, whether natural plant ecotypes re-shape a core “stress responsive microbiome” remains elusive. Considering that drought is the most serious abiotic stress worldwide, we conducted comparative microbiome analysis to study the potential conserved core root microbiome changes upon drought stress in natural Arabidopsis ecotypes. We screened the drought tolerance of 130 worldwide Arabidopsis ecotypes, and chose the extremely drought tolerant and sensitive ecotypes for comparative microbiome studies. We detected diverse differentially abundant ASVs , network driver taxa, as well as key functions pathways shared in diverse ecotypes upon drought stress, indicating the existence of some core drought-responsive microbiome changes. Our work unveiled the existence of a core drought-responsive microbiome and its critical role in enhancing plant fitness. This work also provided a research paradigm for guiding the discovery of stress-alleviating microbiomes in sustainable agriculture using natural ecotypes.
分类： 生物学 >> 植物学 分类： 生物学 >> 微生物学 提交时间： 2023-11-17
摘要： Drought is one of the most serious abiotic stresses which also shifts the composition of root associated microbiomes. However, there is a lack of genetic evidence regarding whether and how plant genetic effects positively reshape drought induced microbiome changes. Root hairs play essential roles in water uptaking, but whether root hairs also orchestrate microbiome re-shaping process during drought stress is unknown. By utilizing genetic mutants with enhanced or decreased root hair densities, we detected a significant effect of plant genetic effect on drought induced microbiome changes. In addition, the hairy mutant (gl2) triggers a deterministic process during drought induced microbiome re-assembly, which further confirms the involvement of host effects in re-shaping drought induced microbiome changes. Rhizobiaceae strains were detected as key biomarker species positively correlated with root hair densities. Moreover, the gl2 mutant also shapes more complex microbiome co-occurrence networks, with more Rhizobiaceae hubs. Our findings unveil the novel roles of root hairs in shaping microbiome structure and network interactions upon drought stress, particularly through regulating the abundance and network centrality of Rhizobiaceae strains. Root hair related mutants also broadly affect root metabolome upon drought stress. Understanding the physiological and microbial ecological basis of host mediated microbiome re-shaping under drought helps develop microbiome engineering approaches to combat climate changes.
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