Subjects: Biology >> Biophysics submitted time 2016-05-11
Xiao, Tengfei
Liu, Lihui
Sun, Yu
Luo, Huaxia
Chen, Runsheng
Xiao, Tengfei
Liu, Lihui
Sun, Yu
Luo, Huaxia
Chen, Runsheng
Xiao, Tengfei
Li, Hongling
Li, Tangping
Wang, Shihua
Zhao, Robert Chunhua
Liu, Lihui
Sun, Yu
Luo, Huaxia
Dalton, Stephen
Abstract： C/EBP alpha is a critical transcriptional regulator of adipogenesis. HowC/EBP alpha transcriptionis itself regulated is poorly understood, however, and remains a key question that needs to be addressed for a complete understanding of adipogenic development. Here, we identify a lncRNA, ADINR(adipogenic differentiation induced noncoding RNA), transcribed from a position similar to 450 bp upstream of the C/EBP alpha gene, that orchestrates C/EBP alpha transcriptionin vivo. Depletion of ADINR leads to a severe adipogenic defect that is rescued by overexpression of C/EBP alpha. Moreover, we reveal that ADINR RNA specifically binds to PA1 and recruits MLL3/4 histone methyl-transferase complexes so as to increase H3K4me3 and decrease H3K27me3 histone modification in the C/EBP alpha locus during adipogenesis. These results show that ADINR plays important roles in regulating the differentiation of human mesenchymal stem cells into adipocytes by modulating C/EBP alpha in cis.
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Awaiting Review
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