摘要: C/EBP alpha is a critical transcriptional regulator of adipogenesis. HowC/EBP alpha transcriptionis itself regulated is poorly understood, however, and remains a key question that needs to be addressed for a complete understanding of adipogenic development. Here, we identify a lncRNA, ADINR(adipogenic differentiation induced noncoding RNA), transcribed from a position similar to 450 bp upstream of the C/EBP alpha gene, that orchestrates C/EBP alpha transcriptionin vivo. Depletion of ADINR leads to a severe adipogenic defect that is rescued by overexpression of C/EBP alpha. Moreover, we reveal that ADINR RNA specifically binds to PA1 and recruits MLL3/4 histone methyl-transferase complexes so as to increase H3K4me3 and decrease H3K27me3 histone modification in the C/EBP alpha locus during adipogenesis. These results show that ADINR plays important roles in regulating the differentiation of human mesenchymal stem cells into adipocytes by modulating C/EBP alpha in cis.
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期刊:
STEM CELL REPORTS
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分类:
生物学
>>
生物物理学
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引用:
ChinaXiv:201605.01236
(或此版本
ChinaXiv:201605.01236V1)
DOI:10.12074/201605.01236V1
CSTR:32003.36.ChinaXiv.201605.01236.V1
- 推荐引用方式:
Xiao, Tengfei,Liu, Lihui,Sun, Yu,Luo, Huaxia,Chen, Runsheng,Xiao, Tengfei,Liu, Lihui,Sun, Yu,Luo, Huaxia,Chen, Runsheng,Xiao, Tengfei,Li, Hongling,Li, Tangping,Wang, Shihua,Zhao, Robert Chunhua,Liu, Lihui,Sun, Yu,Luo, Huaxia,Dalton, Stephen.Long Noncoding RNA ADINR Regulates Adipogenesis by Transcriptionally Activating C/EBP alpha.中国科学院科技论文预发布平台.[ChinaXiv:201605.01236V1]
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