Subjects: Astronomy >> Astrophysical processes submitted time 2022-04-14 Cooperative journals: 《天文研究与技术》
Abstract：太阳黑子是发生在太阳光球层的现象，对太阳耀斑预测具有重要意义。针对类别样本数量不均衡的三分类太阳黑子数据集，提出了双模型集成分类算法。该方法通过一轻一重两个模型，分别承担两个类别的分类任务，再将二者的分类结果进行集成，夹逼出第三个类别的分类结果。实验表明该方法能够减少单个模型在不均衡数据集上出现的过拟合和欠拟合情况带来的不利影响，从新的角度解决了太阳黑子数据集的类别不均衡问题，平均F1分数达到0.931。
Subjects: Biology >> Biochemistry submitted time 2022-04-04
Abstract：
[Objective] The changes in the immune system in urine proteome were observed by injecting bovine serum albumin and aluminum hydroxide adjuvant into rats.
[Methods] In this study, bovine serum albumin and aluminum hydroxide adjuvant were injected into rat thigh muscle, urine was collected, differential proteins were identified by liquid chromatography-mass spectrometry (LC-MS / MS), and biological pathways of differential proteins were analyzed by IPA software to observe the changes of the immune system in rat urinary protein.
[Results] Fifteen rats were intramuscularly injected with normal saline, aluminum hydroxide adjuvant, bovine serum albumin, aluminum hydroxide adjuvant, and bovine serum albumin (BSA) mixture to construct the models of the control group, adjuvant group, BSA group, and mixed group. Comparing the different proteins between different groups to get the relevant biological pathways, it was found that adjuvants can be observed in urine to help bovine serum albumin stimulate the immune system to respond earlier. It was also observed in urine that the mixed group successively stimulated immune-related pathways such as inflammatory response, T cell activation, antigen-presenting cell-related pathways, and B cell-related pathways.
[Discussion] We can observe the changes in the immune system from urine proteome in the early stage, which provides some new clues and a basis for future research on the immune system and even accelerates the research and development of a vaccine.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2022-01-09
Abstract： [Objective] Through the liver cancer-inflammatory cancer model induced by DEN, observe the dynamic change process of urine proteome at different stages of the disease. [Methods] In this study, a rat liver cancer-inflammatory cancer model was constructed by intraperitoneal injection of DEN, urine was collected weekly, and differential proteins were identified by liquid chromatography coupled with mass spectrometry (LC-MS/MS), and the biological pathways of differential proteins were analyzed using IPA software. To observe the changes in urine protein at different stages of the disease in the rat liver cancer-inflammatory cancer model. [Results] Fifteen experimental rats were induced by DEN to construct different stages of the hepatitis-cirrhosis-liver cancer disease model, and each rat had a different disease progression due to individual differences. Each rat has more differential proteins at different disease time points. The differential proteins of different rats are enriched in the same biological pathways related to liver injury, inflammatory lesions, tumor occurrence, and potential therapeutic targets of the tumor. [Discussion] We can observe different changes of urine proteome in each stage of the liver cancer inflammatory cancer model, and different rats will have different disease progression due to individual differences, which suggests that we need to pay more attention to the individualization and accuracy of detection in the future. "
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2020-06-15
Abstract： " "
Peer Review Status:Awaiting Review
Subjects: Biology >> Bioengineering submitted time 2017-07-24 Cooperative journals: 《中国生物工程杂志》
Abstract：为获得具有免疫原性的TK1重组蛋白。通过构建能够表达TK1蛋白的重组菌BL21-pET32a-TK1,采用大肠杆菌pET32a表达系统，优化IPTG浓度、诱导温度、诱导时间使BL21-pET32a-TK1重组菌表达目的蛋白的作用条件最佳。表达产物用镍离子亲和层析纯化获得TK1蛋白，并用SDS-PAGE和western blot进行检测。用TK1重组蛋白免疫BALB/c小鼠制备单克隆抗体，检测蛋白免疫原性。实验结果表明：成功构建能够表达TK1蛋白的重组菌BL21-pET32a-TK1，在37℃条件下，IPTG浓度为0.2mM/L、诱导6h时重组蛋白TK1表达量最高。镍离子亲和层析梯度洗脱在80mM咪唑条件下TK1蛋白纯度最大，灰度分析为87.3%，浓缩后蛋白浓度为5.96mg/ml。用该蛋白制备杂交瘤共获得10株稳定分泌TK1抗体的阳性单克隆细胞株，表明TK1重组蛋白具有较好的免疫原性。本实验成功获得可溶性、抗原活性高、免疫原性强的TK1重组蛋白，为肿瘤科学及临床应用研究提供物质支撑。
Subjects: Biology >> Ecology Subjects: Geosciences >> Marine Sciences submitted time 2017-03-22 Cooperative journals: 《生态学报》
Abstract：江苏海门蛎岈山牡蛎礁是我国目前现存的面积最大的潮间带活体牡蛎礁，但由于人类活动干扰及泥沙淤积等原因，该自然牡蛎礁面积不断缩小，影响着该牡蛎礁的生态功能与保护管理。于2013-2014年开展了首期江苏海门蛎岈山牡蛎礁恢复工程，基于生态监测结果评估该牡蛎礁恢复工程的生态效果。多层礁体上活体牡蛎丰度显著高于单层礁体（P 0.05）。随着礁体的发育，恢复牡蛎礁上大型底栖动物群落的物种丰度并没有增加，但其平均总密度和总生物量均呈现显著性的增长（P 0.05），但显著高于未恢复区（P < 0.05）；恢复牡蛎礁与自然牡蛎礁具有显著不同的大型底栖动物群落（ANOSIM，P=0.001）。研究结果表明该牡蛎礁恢复工程取得初步成功。
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