摘要: The structures of several Bacillus thuringiensis (Bt) insecticidal crystal proteins have been determined by crystallographic methods and a close relationship has been explicated between specific toxicities and conserved three-dimensional architectures. In this study, as a representative of the coleopteran- and hemipteran-specific Cry51A group, the complete structure of Cry51Aa1 protoxin has been determined by X-ray crystallography at 1.65 angstrom resolution. This is the first report of a coleopteran-active Bt insecticidal toxin with high structural similarity to the aerolysin-type beta-pore forming toxins (beta-FFTs). Moreover, study of featured residues and structural elements reveal their possible roles in receptor binding and pore formation events. This study provides new insights into the action of aerolysin-type (beta-PFTs from a structural perspective, and could be useful for the control of coleopteran and hemipteran insect pests in agricultures. (C) 2015 Elsevier Inc. All rights reserved.
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期刊:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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分类:
生物学
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生物物理学
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生物物理、生物化学与分子生物学
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引用:
ChinaXiv:201605.01495
(或此版本
ChinaXiv:201605.01495V1)
DOI:10.12074/201605.01495V1
CSTR:32003.36.ChinaXiv.201605.01495.V1
- 推荐引用方式:
Xu, Chengchen,Meng, Ying,Yu, Ziniu,Sun, Ming,Xu, Chengchen,Chinte, Unmesh,Chen, Lirong,Zhou, Dayong,Rose, John,Adang, Michael J.,Wang, Bi-Cheng,Chinte, Unmesh,Rose, John,Wang, Bi-Cheng,Chinte, Unmesh,Rose, John,Wang, Bi-Cheng,Yao, Qingqing,Bi, Li-Jun,Adang, Michael J..(2016).Crystal structure of Cry51Aa1: A potential novel insecticidal aerolysin-type beta-pore-forming toxin from Bacillus thuringiensis.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.[ChinaXiv:201605.01495]
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