摘要: Membrane receptors at the surface of target cells are key host factors for virion entry; however, it is unknown whether trafficking and secretion of progeny virus requires host intracellular receptors. In this study, we demonstrate that dengue virus (DENV) interacts with KDEL receptors (KDELR), which cycle between the ER and Golgi apparatus, for vesicular transport from ER to Golgi. Depletion of KDELR by siRNA reduced egress of both DENV progeny and recombinant subviral particles (RSPs). Coimmunoprecipitation of KDELR with dengue structural protein prM required three positively charged residues at the N terminus, whose mutation disrupted protein interaction and inhibited RSP transport from the ER to the Golgi. Finally, siRNA depletion of class II Arfs, which results in KDELR accumulation in the Golgi, phenocopied results obtained with mutagenized prME and KDELR knockdown. Our results have uncovered a function for KDELR as an internal receptor involved in DENV trafficking.
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期刊:
CELL REPORTS
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分类:
生物学
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生物物理学
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细胞生物学
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引用:
ChinaXiv:201605.01355
(或此版本
ChinaXiv:201605.01355V1)
DOI:10.12074/201605.01355V1
CSTR:32003.36.ChinaXiv.201605.01355.V1
- 推荐引用方式:
Li, Ming Yuan,Kwok, Kevin,Siu, Yu Lam,Zhang, Jing Shu,Kudelko, Mateusz,Bruzzone, Roberto,Wang, Pei Gang,Li, Ming Yuan,Kwok, Kevin,Siu, Yu Lam,Zhang, Jing Shu,Kudelko, Mateusz,Bruzzone, Roberto,Wang, Pei Gang,Grandadam, Marc,Sayteng, Kouxiong,Lagache, Thibault,Olivo-Marin, Jean-Christophe,Lagache, Thibault,Olivo-Marin, Jean-Christophe,Bruzzone, Roberto,Kudelko, Mateusz,Qin, Cheng Feng,Wang, Pei Gang.(2016).KDEL Receptors Assist Dengue Virus Exit from the Endoplasmic Reticulum.中国科学院科技论文预发布平台.[ChinaXiv:201605.01355]
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