摘要: Esophageal Squamous Cell Carcinoma (ESCC) is among the most common malignant cancers worldwide. In the past, extensive efforts have been made to characterize the involvement of protein-coding genes in ESCC tumorigenesis but few for long noncoding RNAs (lncRNAs). To investigate the transcriptome profile and functional relevance of lncRNAs, we performed an integrative analysis of a customized combined lncRNA-mRNA microarray and RNA-seq data on ESCCs and matched normal tissues. We identified numerous lncRNAs that were differentially expressed between the normal and tumor tissues, termed "ESCC-associated lncRNAs (ESCALs)", of which, the majority displayed restricted expression pattern. Also, a subset of ESCALs appeared to be associated with ESCC patient survival. Gene set enrichment analysis (GSEA) further suggested that over half of the ESCALs were positively- or nelgativelyassociated with metastasis. Among these, we identified a novel nuclear-retained lncRNA, named Epist, which is generally highly expressed in esophagus, and which is down-regulated during ESCC progression. Epist over-expression and knockdown studies further suggest that Epist inhibits the metastasis, acting as a tumor suppressor in ESCC. Collectively, our analysis of the ESCC transcriptome identified the potential tumor suppressing lncRNA Epist, and provided a foundation for future efforts to identify functional lncRNAs for cancerous therapeutic targeting.
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期刊:
ONCOTARGET
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分类:
生物学
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生物物理学
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肿瘤学
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引用:
ChinaXiv:201605.01284
(或此版本
ChinaXiv:201605.01284V1)
DOI:10.12074/201605.01284V1
CSTR:32003.36.ChinaXiv.201605.01284.V1
- 推荐引用方式:
Wei, Guifeng,Luo, Huaxia,Sun, Yu,Tian, Liqing,Liu, Wei,Liu, Lihui,Luo, Jianjun,Chen, Runsheng,Li, Jiagen,He, Jie.(2016).Transcriptome profiling of esophageal squamous cell carcinoma reveals a long noncoding RNA acting as a tumor suppressor.中国科学院科技论文预发布平台.[ChinaXiv:201605.01284]
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