分类： 生物学 >> 生物物理学 >> 衰老生物学 提交时间： 2016-05-05
摘要： microRNA-9 (miR-9) is highly expressed in the nervous system across species and plays essential roles in neurogenesis and axon growth; however, little is known about the mechanisms that link miR-9 with dendrite growth. Using an in vivo model of Drosophila class I dendrite arborization (da) neurons, we show that miR-9a, a Drosophila homolog of mammalian miR-9, downregulates the cadherin protein Flamingo (Fmi) thereby attenuating dendrite development in a non-cell autonomous manner. In miR-9a knockout mutants, the dendrite length of a sensory neuron ddaE was significantly increased. Intriguingly, miR-9a is specifically expressed in epithelial cells but not in neurons, thus the expression of epithelial but not neuronal Fmi is greatly elevated in miR-9a mutants. In contrast, overexpression of Fmi in the neuron resulted in a reduction in dendrite growth, suggesting that neuronal Fmi plays a suppressive role in dendrite growth, and that increased epithelial Fmi might promote dendrite growth by competitively binding to neuronal Fmi. Fmi has been proposed as a G protein-coupled receptor (GPCR), we find that neuronal G protein Gq (Gq), but not Go, may function downstream of Fmi to negatively regulate dendrite growth. Taken together, our results reveal a novel function of miR-9a in dendrite morphogenesis. Moreover, we suggest that Gq might mediate the intercellular signal of Fmi in neurons to suppress dendrite growth. Our findings provide novel insights into the complex regulatory mechanisms of microRNAs in dendrite development, and further reveal the interplay between the different components of Fmi, functioning in cadherin adhesion and GPCR signalling. (c) 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 225-237, 2016
分类： 生物学 >> 生物物理学 提交时间： 2016-05-12
Wang, Yan
Wang, Mingxia
Wang, Jian
Xu, Tao
Yin, Sanwen
Jang, Richard
Xue, Zhidong
Jang, Richard
Xu, Tao
摘要： Neuropeptides play a variety of roles in many physiological processes and serve as potential therapeutic targets for the treatment of some nervous-system disorders. In recent years, there has been a tremendous increase in the number of identified neuropeptides. Therefore, we have developed NeuroPep, a comprehensive resource of neuropeptides, which holds 5949 non-redundant neuropeptide entries originating from 493 organisms belonging to 65 neuropeptide families. In NeuroPep, the number of neuropeptides in invertebrates and vertebrates is 3455 and 2406, respectively. It is currently the most complete neuropeptide database. We extracted entries deposited in UniProt, the database (www.neuropeptides.nl) and NeuroPedia, and used text mining methods to retrieve entries from the MEDLINE abstracts and full text articles. All the entries in NeuroPep have been manually checked. 2069 of the 5949 (35%) neuropeptide sequences were collected from the scientific literature. Moreover, NeuroPep contains detailed annotations for each entry, including source organisms, tissue specificity, families, names, post-translational modifications, 3D structures (if available) and literature references. Information derived from these peptide sequences such as amino acid compositions, iso-electric points, molecular weight and other physicochemical properties of peptides are also provided. A quick search feature allows users to search the database with keywords such as sequence, name, family, etc., and an advanced search page helps users to combine queries with logical operators like AND/OR. In addition, user-friendly web tools like browsing, sequence alignment and mapping are also integrated into the NeuroPep database.
分类： 生物学 >> 生物物理学 提交时间： 2016-05-12
Yang, Mingkun
Wang, Yan
Chen, Ying
Mo, Ran
Ge, Feng
Cheng, Zhongyi
Gu, Jing
Deng, Jiaoyu
Wang, Xude
Bi, Lijun
Chen, Chuangbin
摘要： Mycobacterium tuberculosis (Mtb), the causative agent of human tuberculosis, remains one of the most prevalent human pathogens and a major cause of mortality worldwide. Metabolic network is a central mediator and defining feature of the pathogenicity of Mtb. Increasing evidence suggests that lysine succinylation dynamically regulates enzymes in carbon metabolism in both bacteria and human cells; however, its extent and function in Mtb remain unexplored. Here, we performed a global succinylome analysis of the virulent Mtb strain H37Rv by using high accuracy nano-LC-MS/MS in combination with the enrichment of succinylated peptides from digested cell lysates and subsequent peptide identification. In total, 1545 lysine succinylation sites on 626 proteins were identified in this pathogen. The identified succinylated proteins are involved in various biological processes and a large proportion of the succinylation sites are present on proteins in the central metabolism pathway. Site-specific mutations showed that succinylation is a negative regulatory modification on the enzymatic activity of acetylCoA synthetase. Molecular dynamics simulations demonstrated that succinylation affects the conformational stability of acetyl-CoA synthetase, which is critical for its enzymatic activity. Further functional studies showed that CobB, a sirtuin-like deacetylase in Mtb, functions as a desuccinylase of acetyl-CoA synthetase in in vitro assays. Together, our findings reveal widespread roles for lysine succinylation in regulating metabolism and diverse processes in Mtb. Our data provide a rich resource for functional analyses of lysine succinylation and facilitate the dissection of metabolic networks in this life-threatening pathogen.
分类： 核科学技术 >> 粒子加速器 提交时间： 2021-12-31
Ma, Zhen-Yu
Zhao, Shen-Jie
Liu, Xu-Ming
Yu, Yue-Chao
Jiang, Hong-Ru
Zheng, Xiang
Chang, Qiang
Zhang, Zi-Gang
Xu, Kai
Wang, Yan
Zhao, Yu-Bin
Hou, Hong-Tao
摘要： The Shanghai High Repetition Rate XFEL and Extreme Light Facility (SHINE) project will use 600 1.3 GHz fundamental power couplers, which are modified based on TTF-III power couplers, for continuous wave operation with input power up to approximately 7 kW. The first batch of 20 sets of 1.3 GHz coupler prototypes were fabricated from three domestic manufacturers for the SHINE project. To better characterize the radio frequency conditioning phenomena for validating the performance of power couplers, a room temperature test stand was designed, constructed, and commissioned for the SHINE 1.3 GHz power couplers. In addition, a horizontal test cryostat was built to test the 1.3 GHz superconducting cavities, fundamental power couplers, tuners, and other components as a set. The results of these tests indicate that the 1.3 GHz couplers are capable of handling up to 14 kW continuous waves. Herein, the main aspects of the radio frequency design and construction of the test stand, along with the test results of the high-power conditioning of the 1.3 GHz couplers, are described.
分类： 生物学 >> 生态学 提交时间： 2017-11-17
Li, L (Li, Li)
Zhang, QJ (Zhang, Quanjun)
Yang, HQ (Yang, Huaqiang)
Zou, QJ (Zou, Qingjian)
Lai, CD (Lai, Chengdan)
Jiang, F (Jiang, Fei)
Zhao, P (Zhao, Ping)
Luo, ZW (Luo, Zhiwei)
Yang, JY (Yang, Jiayin)
Chen, Q (Chen, Qian)
Wang, Y (Wang, Yan)
Newsome, PN (Newsome, Philip N.)
Frampton, J (Frampton, Jon)
Maxwell, PH (Maxwell, Patrick H.)
Li, WJ (Li, Wenjuan)
Chen, SH (Chen, Shuhan)
Wang, DY (Wang, Dongye)
Siu, TS (Siu, Tak-Shing)
Tam, S (Tam, Sidney)
Tse, HF (Tse, Hung-Fat)
摘要： From the (a)CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China, and Guangzhou Medical University, Guangzhou 511436, China, (b)CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China, (c)Laboratory of RNA, Chromatin, and Human Disease, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China, (d)Cardiology Division, Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong SAR, China, (e)Hong Kong-Guangdong Stem Cell and Regenerative Medicine Research Centre, The University of Hong Kong and Guangzhou Institutes of Biomedicine and Health, Hong Kong SAR, China, (f)Department of Ophthalmology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China, (g)State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Guangdong Provincial Research Center for Liver Fibrosis, Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital and (h)Biomedical Research Center, Southern Medical University, Guangzhou 510515, China, (i)Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, (j)National Institute for Health Research (NIHR) Birmingham Liver Biomedical Research Unit and Centre for Liver Research, and (k)Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, United Kingdom, (l)Cambridge Institute for Medical Research, Wellcome Trust/Medical Research Council (MRC) Building, Cambridge CB2 0XY, United Kingdom, mDepartment of Clinical Biochemistry Unit, Queen Mary Hospital, Hong Kong SAR, China, (n)Department of Medicine, University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, Guangdong, China, and (o)Laboratory of Metabolism and Cell Fate, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, Guangdong, China
点击量
待评议
点击量
下载量
评论
