Urine proteomic analysis of rat startle model

Abstract: The fear of startle is generated in the brain, and urine proteomics was used to explore whether this fear can be detected in the urine. The combination of natural enemy odor and sound stimulation were used to establish a rat model of startle. Urine samples were collected before and after startle and proteomic analysis was performed. The results showed that 22 differential proteins were identified after startle, and the biological pathways enriched in these differential proteins were related to neurotransmitter transport and glucose transmembrane transport, which may be the manifestations of nervous tension caused by startle. Before-after study in single rat was performed and found that there was one differential protein identified samely in five rats, in addition, 19 differential proteins were identified samely in four of five rats, and these proteins were associated with the change of nerve, motion, metabolism and blood pressure, which include catalytic subunits and regulatory subunits of glutamate-cysteine ligase, which realted to the function of startle. These results laid the foundation for the research of startle mechanism, and to find medicine for the treatment of psychological terror target provides a new method. At the same time, it fully illustrates the sensitivity of urine and opens up a new field for the exploration of urine.