To observe the dynamic changes of the urinary proteome in rats during immunization

Abstract: [Objective] The changes in the immune system in urine proteome were observed by injecting bovine serum albumin and aluminum hydroxide adjuvant into rats.

[Methods] In this study, bovine serum albumin and aluminum hydroxide adjuvant were injected into rat thigh muscle, urine was collected, differential proteins were identified by liquid chromatography-mass spectrometry (LC-MS / MS), and biological pathways of differential proteins were analyzed by IPA software to observe the changes of the immune system in rat urinary protein.

[Results] Fifteen rats were intramuscularly injected with normal saline, aluminum hydroxide adjuvant, bovine serum albumin, aluminum hydroxide adjuvant, and bovine serum albumin (BSA) mixture to construct the models of the control group, adjuvant group, BSA group, and mixed group. Comparing the different proteins between different groups to get the relevant biological pathways, it was found that adjuvants can be observed in urine to help bovine serum albumin stimulate the immune system to respond earlier. It was also observed in urine that the mixed group successively stimulated immune-related pathways such as inflammatory response, T cell activation, antigen-presenting cell-related pathways, and B cell-related pathways.

[Discussion] We can observe the changes in the immune system from urine proteome in the early stage, which provides some new clues and a basis for future research on the immune system and even accelerates the research and development of a vaccine.