Subjects: Physics >> Nuclear Physics submitted time 2023-12-06
Abstract: High spin states of 94Nb have been studied with the 82Se(18O, p5n)94Nb fusion evaporation reaction at anincident beam energy of 82and 88 MeV. The level scheme of 94Nbhas been modified and extended with 15 newγrays. Based on γ-γcoincidence relationships,DCO ratios and linear polarization measurements, the new level structures in 94Nb have been interpreted in terms of the shell model calculations performed in the configuration space π(1f5/2, 2p3/2, 2p1/2, 1g9/2 ) for the protons and ν(2p1/2, 1g9/2, 1g7/2, 2d5/2 ) for the neutrons.
Peer Review Status:Awaiting Review
Subjects: Biology >> Bioengineering submitted time 2017-11-13 Cooperative journals: 《中国生物工程杂志》
Abstract:目的:研究STAT3在肝癌细胞SK-Hep1对sorafenib抗性中的作用,并探讨STAT3基因沉默在增强sorafenib肝癌疗效中的作用。 方法:应用基于shRNA的基因沉默技术在肝癌细胞SK-Hep1中敲减STAT3;用CCK8法检测细胞的生长情况与对sorafenib的敏感性;蛋白印迹法(Western blot)检测STAT3、p-STAT3(Y705)、p-STAT3(S727)以及其下游蛋白的表达变化。 结果:成功构建了STAT3敲减的细胞株SK-Hep1-shSTAT3。该细胞中STAT3蛋白表达降低,细胞增殖明显受到抑制。Sorafenib处理下调了STAT3磷酸化水平及其下游蛋白Mcl-1和CyclinD1的表达。STAT3基因敲减的SK-Hep1细胞中,sorafenib敏感性增强。 结论:基于shRNA的STAT3基因沉默能明显抑制SK-Hep1细胞增殖,提高细胞对sorafenib的敏感性,有望成为提高sorafenib抗肝癌疗效的一种新手段。