CodY在单核细胞增生李斯特菌运动和毒力方面的作用

Subjects: Biology >> Bioengineering submitted time 2017-07-24 Cooperative journals: 《中国生物工程杂志》

张颖 汤雨倩 沈怡 罗勤

Abstract： Abstract: [Objective] To explore the function of the transcription factor CodY on flagellar motility, bacterial virulence in Listeria monocytogenes. [Methods] The codY gene on the L.monocytogenes chromosome was knocked out by homologous recombination and the strain of the deleted codY gene was successfully complemented by the codY gene construct; the results were evaluated by observation of the motility of flagellar movement, the transcriptional expression of flagellum-related genes with RT-qPCR. The effects of wild-type strain EGDe and CodY-deficient strain on bacterial hemolytic activity, semi-lethal dose of Helicoverpa armigera and the main virulence factor LLO(coding gene hly) and virulence regulatory protein PrfA(coding gene prfA) transcription expression profiles were compared. [Results] Compared with the wild-type strain, the CodY-deficient strain showed a significant reduction in the flagellar movement, the hemolytic activity, as well as the transcriptional expression of flagellum-related genes and the major virulence genes hly and prfA(P≤0.01); while the semi-lethal dose on Helicoverpa armigera (Hübner) was increased 5.8 fold. [Conclusion] The CodY plays an important role in flagellar motility and transcription regulation of bacterial virulence in Listeria monocytogenes.

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