分类： 物理学 >> 普通物理:统计和量子力学,量子信息等 提交时间： 2017-05-02
Hou, RX [Hou, Ruixia][ 1 ]
Nie, L [Nie, Lei][ 1 ]
Du, GL [Du, Gaolai][ 1 ]
Xiong, XP [Xiong, Xiaopeng][ 2 ]
Fu, J [Fu, Jun][ 1 ]
摘要： This paper aims to investigate the synergistic effects of natural polysaccharides and inorganic nanopar- ticles on cell adhesion and growth on intrinsically cell non-adhesive polyvinyl alcohol (PVA) hydrogels. Previously, we have demonstrated that Fe2O3 and hydroxyapatite (nHAP) nanoparticles are effective in increasing osteoblast growth on PVA hydrogels. Herein, we blended hyaluronic acid (HA) and chondroitin sulfate (CS), two important components of cartilage extracellular matrix (ECM), with Fe2O3/nHAP/PVA hydrogels. The presence of these natural polyelectrolytes dramatically increased the pore size and the equilibrium swelling ratio (ESR) while maintaining excellent compressive strength of hydrogels. Chon- drocytes were seeded and cultured on composite PVA hydrogels containing Fe2 O3 , nHAP and Fe2 O3 /nHAP hybrids and Fe2O3/nHAP with HA or CS. Confocal laser scanning microscopy (CLSM) and cell counting kit-8 (CCK-8) assay consistently confirmed that the addition of HA or CS promotes chondrocyte adhesion and growth on PVA and composite hydrogels. Particularly, the combination of HA and CS exhibited fur- ther promotion to cell adhesion and proliferation compared with any single polysaccharide. The results demonstrated that the magnetic composite nanoparticles and polysaccharides provided synergistic pro- motion to cell adhesion and growth. Such polysaccharide-augmented composite hydrogels may have potentials in biomedical applications.
分类： 物理学 >> 普通物理:统计和量子力学,量子信息等 提交时间： 2017-05-02
Du, GL [Du, Gaolai][ 1 ]
Gao, GR [Gao, Guorong][ 1 ]
Hou, RX [Hou, Ruixia][ 1 ]
Cheng, YJ [Cheng, Yajun][ 1 ]
Chen, T [Chen, Tao][ 1 ]
Fu, J [Fu, Jun][ 1 ]
Fei, B [Fei, Bin][ 2 ]
摘要： Supertough biomimetic hydrogels have been fabricated through in situ synthesis and guided assembling of positively charged conjugated polymer belts by using a parent poly(2-acrylamido-2-methylpropanesulfonic acid)/poly- (acrylamide) double network (PAMPS/PAAm DN) gel template. The inter- penetrating structures of the poly(3,4-ethylenedioxythiophene) (PEDOT) belt mesh and PAMPS/PAAm host network have been confirmed by SEM, CLSM, and Raman spectroscopy. The presence of PEDOT belts improves the Young’s modulus, compressive strength, and toughness of the biomimetic (BM) hydrogels, in comparison to the parent DN gels. Cyclic tensile (300% strain) and compressive (even 90% strain) loadings demonstrate extraordinary fatigue resistance of these BM gels. Upon ten cycles, the compressive toughness remained about 1000 J m−2, which is comparable to that of articular cartilage. The internal fracture behavior and fatigue resistance of these biomimetic interpenetrating hydrogels are further investigated. These extremely tough and fatigue resistant BM hydrogels may find applications as promising substitutes for load-bearing tissues.
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