Subjects: Library Science，Information Science >> Information Science submitted time 2023-04-01 Cooperative journals: 《图书情报工作》
Abstract： [Purpose/significance] Knowledge graph has become a new form of public digital cultural resources organization. Using knowledge graph technology to enable the Knowledge Q & A service of red historical figures and improve user interaction experience is of great significance to the development and utilization of red historical resources.[Method/process] On the basis of combing the related research of digital resource organization and Knowledge Q & A system of historical figures, the paper constructed the knowledge graph schema and KBQA architecture of red historical figures, and then built the model of Q & A from five aspects of data acquisition, knowledge extraction, knowledge fusion, graph generation and Knowledge Q & A.This paper took the red historical figures digitalresources of Shanghai University(1922-1927) as an example for empirical research.[Result/conclusion] The Knowledge Q & A service architecture designed in this paper has advantages in semi-automatic graph construction, knowledge reasoning and intelligent interaction of digital resources of red historical figures, and improves the user knowledge service experience.
Subjects: Biology >> Botany >> Applied botany submitted time 2018-10-26 Cooperative journals: 《广西植物》
Abstract：海南龙血树是国产血竭的主要基源植物，其血竭主要化学成分为类黄酮化合物。目前对血竭的研究集中在化学成分及药理活性分析，对血竭形成的分子机制并不了解。WD40转录因子在类黄酮的积累中具有重要的调控作用。本研究根据海南龙血树转录组数据，利用RT-PCR技术在海南龙血树中克隆了一个WD40基因DcWD40-1，该基因包含一个1 353 bp的开放阅读框，编码450个氨基酸，蛋白质分子量50.77 kD，理论等电点5.71。生物信息学分析显示，DcWD40-1属于WD40蛋白家族成员，具有5个保守的WD40结构域，和其他植物WD40蛋白同源性高，保守性强。利用Genome Walking方法分离了1 503 bp的DcWD40-1启动子序列，该区域具有典型真核生物启动子结构特征，并含有多个应答激素和胁迫的响应元件。表达分析显示，血竭诱导剂能够诱导DcWD40-1的表达，DcWD40-1的变化与血竭形成及类黄酮积累正相关。此外，DcWD40-1也能对茉莉酸甲酯、细胞分裂素、油菜素内酯和UV-B处理做出积极响应。本结果为进一步研究DcWD40-1在类黄酮生物合成中的潜在功能和作用机制奠定基础。
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