分类: 天文学 >> 天文仪器与技术 分类: 光学 >> 仪器仪表、测量和计量 分类: 光学 >> 相干和统计光学 提交时间: 2023-06-28
摘要: Co-phase and co-focus detection is one of the key technologies for large-aperture segmented mirror telescopes. In this paper, a new edge sensor based on fringes of equal thickness is developed, which can detect each segment's relative piston, tilt, and tip errors from the interferograms. Based on the co-focus demand for many ground-based seeing limited segmented mirror telescopes, an edge sensor prototype based on such a principle is built and applied in the indoor segmented mirror experiment system in the lab. According to the co-focus requirement of the Large Sky Area Multi-Object Fiber Spectroscopic Telescope, many simulations and experiments are carried out for co-focus error detection of the segmented mirror system. Experiment results show that the co-focus accuracy is better than 0.02 rms, which can meet the co-focus requirements of most large or extremely large segmented mirror astronomical telescopes.
分类: 生物学 >> 生物物理学 提交时间: 2016-05-15
摘要: Shiga-like toxins (Stxs), produced by pathogenic Escherichia coli, are a major virulence factor involved in severe diseases in human and animals. These toxins are ribosome-inactivating proteins, and treatment for diseases caused by them is not available. Therefore, there is an urgent need for agents capable of effectively targeting this lethal toxin. In this study, we identified baicalin, a flavonoid compound used in Chinese traditional medicine, as a compound against Shiga-like toxin 2 (Stx2). We found that baicalin significantly improves renal function and reduces Stx2-induced lethality in mice. Further experiments revealed that baicalin induces the formation of oligomers by the toxin by direct binding. We also identified the residues important for such interactions and analyzed their roles in binding baicalin by biophysical and biochemical analyses. Our results establish baicalin as a candidate compound for the development of therapeutics against diseases caused by Stxs.
分类: 生物学 >> 生物物理学 >> 生物物理、生物化学与分子生物学 提交时间: 2016-05-12
摘要: Toxic ribosome-inactivating proteins abolish cell viability by inhibiting protein synthesis. Ricin, a member of these lethal proteins, is a potential bioterrorism agent. Despite the grave challenge posed by these toxins to public health, post-exposure treatment for intoxication caused by these agents currently is unavailable. In this study, we report the identification of baicalin extracted from Chinese herbal medicine as a compound capable of inhibiting the activity of ricin. More importantly, post-exposure treatment with baicalin significantly increased the survival of mice poisoned by ricin. We determined the mechanism of action of baicalin by solving the crystal structure of its complex with the A chain of ricin (RTA) at 2.2 angstrom resolution, which revealed that baicalin interacts with two RTA molecules at a novel binding site by hydrogen bond networks and electrostatic force interactions, suggesting its role as molecular glue of the RTA. Further biochemical and biophysical analyses validated the amino acids directly involved in binding the inhibitor, which is consistent with the hypothesis that baicalin exerts its inhibitory effects by inducing RTA to form oligomers in solution, a mechanism that is distinctly different from previously reported inhibitors. This work offers promising leads for the development of therapeutics against ricin and probably other ribosome-inactivating proteins.