Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Other Disciplines of Agriculture, Forestry,Livestock & Aquatic Products Science submitted time 2023-02-17 Cooperative journals: 《智慧农业（中英文）》
Abstract： Phytophthora strawberries, as a kind of plant pathogenic fungi, can cause strawberry skin and crown rot without safe and effective treatment, which affect the economic benefits of planting strawberries. Therefore, it is urgent to use low-cost diagnostic methods to achieve early prevention. Strawberry plants infected with Phytophthora cactorum would release a unique organic volatile gas, 4-ethylphenol, with a concentration ranging from 1.12 to 22.56 mg/kg, which could be used as a marker gas for rapid diagnosis of the disease. In this study, semiconducting single-walled carbon nanotubes (SWNT) and field effect sensors (FET) were used to prepare semiconductor field effect gas sensors (SWNT/FET) without selectivity. And then the metal porphyrin MnOEP with high sensitivity and selectivity to 4-ethylphenol was immoblized on the SWNT's surface to obtain MnOEP-SWNT/FET. MnOEP-SWNT/FET has the advantages of low cost, low power consumption, small size, high sensitivity and easy integration, which can effectively overcome the shortcomings of gas chromatography-mass spectrometry, high-performance liquid chromatography and other analytical methods. By comparing the sensitivity and selectivity of different sensors, MnOEP-SWNT/FET is very suitable for real-time monitoring of 4-ethylphenol. The key reasons for the high sensitivity and selectivity are: MnOEP is a macromolecular heterocyclic compound formed by four pyrrole rings connected together by methylene and manganese ion(Mn), each pyrrole ring consists of four carbons and one nitrogen, and all nitrogen atoms inside the ring form a central cavity; the coordination metal ions of MnOEP are in an unsaturated state, gas molecules can interact with the central metal ions through van der Waals force and hydrogen bond at the axial position of MnOEP to change their own optical or electrical properties. MnOEP-SWNT/FET was studied by Raman spectrum, UV spectrum and voltammetry. The physical and chemical properties were analyzed and the detection conditions were optimized to improve the gas sensitivity of MnOEPSWNT/ FET to 4-ethylphenol. Under the optimal detection conditions, MnOEP-SWNT/FET has a good linear relationship with 0.25% ~100% saturated vapor of 4-ethylphenol (20 ℃) and the detection limit is 0.15% saturated vapor of 4-ethylphenol. The relative standard error of different concentrations was less than 10%. By measuring the actual samples, it has high detection accuracy for strawberry plants infected with Phytophthora, but it still exists false positive for healthy strawberry.
Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Other Disciplines of Agriculture, Forestry,Livestock & Aquatic Products Science submitted time 2023-02-17 Cooperative journals: 《智慧农业（中英文）》
Abstract： The production mode of livestock breeding has changed from extensive to intensive, and the production level is improved. However, low labor productivity and labor shortage have seriously restricted the rapid development of China's livestock breeding industry. As a new intelligent agricultural machinery equipment, agricultural robot integrates advanced technologies, such as intelligent monitoring, automatic control, image recognition technology, environmental modeling algorithm, sensors, flexible execution, etc. Using modern information and artificial intelligence technology, developing livestock feeding and pushing robots, realizing digital and intelligent livestock breeding, improving livestock breeding productivity are the main ways to solve the above contradiction. In order to deeply analyze the research status of robot technology in livestock breeding, products and literature were collected worldwide. This paper mainly introduced the research progress of livestock feeding robot from three aspects: Rail feeding robot, self-propelled feeding robot and pushing robot, and analyzed the technical characteristics and practical application of feeding robot.The rail feeding robot runs automatically along the fixed track, identifies the target animal, positions itself, and accurately completes feed delivery through preset programs to achieve accurate feeding of livestock. The self-propelled feeding robot can walk freely in the farm and has automatic navigation and positioning functions. The system takes single chip microcomputer as the control core and realizes automatic walking by sensor and communication module. Compared with the rail feeding robot, the feeding process is more flexible, convenient and technical, which is more conducive to the promotion and application of livestock farms. The pushing robot will automatically push the feed to the feeding area, promote the increase of feed intake of livestock, and effectively reduce the labor demand of the farm. By comparing the feeding robots of developed countries and China from two aspects of technology and application, it is found that China has achieved some innovation in technology, while developted countries do better in product application. The development of livestock robot was prospected. In terms of strategic planning, it is necessary to keep up with the international situation and the trend of technological development, and formulate the agricultural robot development strategic planning in line with China's national conditions. In terms of the development of core technologies, more attention should be paid to the integration of information perception, intelligent sensors and deep learning algorithms to realize human-computer interaction. In terms of future development trends, it is urgent to strengthen innovation, improve the friendliness and intelligence of the robot, and improve the learning ability of the robot. To sum up, intelligent livestock feeding and pushing machine operation has become a cutting-edge technology in the field of intelligent agriculture, which will surely lead to a new round of agricultural production technology reform, promote the transformation and upgrading of China's livestock industry. .
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