Subjects: Biology >> Botany >> Applied botany submitted time 2019-06-17 Cooperative journals: 《广西植物》
Abstract：非结构性碳水化合物（NSC）作为光合作用的主要产物，其含量多少可反映植物整体的碳收支平衡关系及对外界环境胁迫的适应策略。该文采用改进的苯酚-浓硫酸法对福建省平潭岛海岸处于生长旺盛期的3种典型沙生植物木麻黄、单叶蔓荆和老鼠艻茎叶的NSC及其组分含量进行测定，结果表明：（1）单叶蔓荆叶片、茎干可溶性糖含量均最大，分别为137.83±3.75 mg•g-1 和90.74±2.65 mg•g-1，老鼠艻叶片、茎干淀粉含量均最大，分别为105.41±2.49 mg•g-1和120.48±7.67 mg•g-1，二者茎叶NSC含量均大于木麻黄，且全部达到显著水平（P＜0.05）。（2）沙堆表面老鼠艻叶片NSC及组分含量从雏形、发育到稳定阶段变化不明显，但茎干NSC及组分含量在雏形阶段沙堆表面显著大于其它两个演替阶段（P＜0.05），且依次呈下降趋势。（3）从幼龄林、中龄林到成龄林阶段，木麻黄NSC含量呈现为先上升后下降趋势，其中成龄林叶片可溶性糖、淀粉和NSC含量、茎干可溶性糖和NSC含量均显著低于其它两个生长阶段（P＜0.05）。（4）不同沙生植物对环境胁迫的适应策略各异，海岸沙丘表面植被恢复应充分考虑物种特性及其更新。
Subjects: Biology >> Botany >> Applied botany submitted time 2018-09-07 Cooperative journals: 《广西植物》
Abstract： Quantification of non-structural carbohydrates (NSC) contents of plants is of great significance to the exploration of its growth and adaptive strategies. However, various pretreating methods often lead to the poor comparability among different results. In this paper, four typical psammophytes on the coast of South China including Casuarina equisetifolia, Spinifex littoreus, Ipomoea pes-caprae and Sesuvium portulacastrum were studied, and the effects of pretreating methods on the determination of non-structural carbohydrates contents of plants by phenol-concentrated sulfuric acid method were analyzed, including the existence of the peel and mesh size. The results showed that: (1) When peeled, NSC, soluble sugar and starch contents of the Casuarina equisetifolia samples all had a decreasing trend compared with the control group, especially significant in the soluble sugar contents of the young-aged forest and the middle-aged forest(P<0.05),butmade nodifference in both theirstarch contentsand all componentcontentsin adult ones. The smaller the age of samples, the greater the effect of peeling pretreatment on NSC andits componentcontentsin trees,especially affectedonsoluble sugar,so we suggestto preserve tree bark when samples processed. (2) The contents of NSC and its components of different psammophytes’s branches (stem) all raised with the increase of mesh size, and there were significant differences between the Casuarina equisetifolia and Spinifex littoreus’branches (stem) samples which passed through the 100 and 18 mesh sieve (P<0.05). Except the Spinifex littoreus, all psammophytes’ leaves (assimilating branches) samples hadn’t changed much under different mush sizes and had no significant difference. The higher the content of mechanical tissue in the samples, the more significant difference in the content of NSC and its components extracted by different mesh size, considering the difficulty in obtaining samples and the accuracy of the determination of NSC content, thus we suggest that all branches and leaves of psammophytes should be processed by 100 mesh sieve. The conclusion of this study can guide the sample processing methods of related research in South China Coast, standardize experimental methods andimprove thecomparabilitybetweendifferentstudies.
Hits
Hits
Downloads
Comment