Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Other Disciplines of Agriculture, Forestry,Livestock & Aquatic Products Science submitted time 2023-02-17 Cooperative journals: 《智慧农业（中英文）》
Abstract： Aiming at the difficulty of accurately extract the phenotypic traits of plants and organs from images or point clouds caused by the multiple tillers and serious cross-occlusion among organs of wheat plants, to meet the needs of accurate phenotypic analysis of wheat plants, three-dimensional (3D) digitization was used to extract phenotypic parameters of wheat plants. Firstly, digital representation method of wheat organs was given and a 3D digital data acquisition standard suitable for the whole growth period of wheat was formulated. According to this standard, data acquisition was carried out using a 3D digitizer. Based on the definition of phenotypic parameters and semantic coordinates information contained in the 3D digitizing data, eleven conventional measurable phenotypic parameters in three categories were quantitative extracted, including lengths, thicknesses, and angles of wheat plants and organs. Furthermore, two types of new parameters for shoot architecture and 3D leaf shape were defined. Plant girth was defined to quantitatively describe the looseness or compactness by fitting 3D discrete coordinates based on the least square method. For leaf shape, wheat leaf curling and twisting were defined and quantified according to the direction change of leaf surface normal vector. Three wheat cultivars including FK13, XN979, and JM44 at three stages (rising stage, jointing stage, and heading stage) were used for method validation. The Open3D library was used to process and visualize wheat plant data. Visualization results showed that the acquired 3D digitization data of maize plants were realistic, and the data acquisition approach was capable to present morphological differences among different cultivars and growth stages. Validation results showed that the errors of stem length, leaf length, stem thickness, stem and leaf angle were relatively small. The R2 were 0.93, 0.98, 0.93, and 0.85, respectively. The error of the leaf width and leaf inclination angle were also satisfactory, the R2 were 0.75 and 0.73. Because wheat leaves are narrow and easy to curl, and some of the leaves have a large degree of bending, the error of leaf width and leaf angle were relatively larger than other parameters. The data acquisition procedure was rather time-consuming, while the data processing was quite efficient. It took around 133 ms to extract all mentioned parameters for a wheat plant containing 7 tillers and total 27 leaves. The proposed method could achieve convenient and accurate extraction of wheat phenotypes at individual plant and organ levels, and provide technical support for wheat shoot architecture related research.
Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Other Disciplines of Agriculture, Forestry,Livestock & Aquatic Products Science submitted time 2023-02-17 Cooperative journals: 《智慧农业（中英文）》
Abstract： Plant micro-phenotype mainly refers to the phenotypic information at the tissue, cell, and subcellular levels, which is an important part of plant phenomics research. In view of the problems of low efficiency, large error, and few traits of traditional methods for detecting kernel microscopic traits, Micro-CT scanning technology was used to carry out precise identification of micro-phenotype on 11 varieties of maize kernels. A total of 34 microscopic traits were obtained based on CT sequence images of 7 tissues, including seed, embryo, endosperm, cavity, subcutaneous cavity, endosperm cavity and embryo cavity. Among the 34 microscopic traits, 4 traits, including endosperm cavity surface area, kernel volume, endosperm volume ratio and endosperm cavity specific surface area, were significantly different among maize types (P-value<0.05). The surface area of endosperm cavity and kernel volume of common maize were significantly higher than those of other types of maize. The specific surface area of endosperm cavity of high oil maize was the largest. The endosperm cavity of sweet corn had the smallest specific surface area. The endosperm volume ration of popcorn was the largest. Furthermore, 34 traits were used for One-way ANOVA and cluster analysis, and 11 different maize varieties were divided into four categories, of which the first category was mainly common maize, the second category was mainly popcorn, the third category was sweet corn, and the fourth category was high oil maize. The results indicated that Micro-CT scanning technology could not only achieve precise identification of micro-phenotype of maize kernels, but also provide supports for kernel classification and variety detection, and so on.
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