分类: 生物学 >> 植物学 >> 植物生物化学、植物生物物理学 提交时间: 2016-05-03
摘要: The role of plant tyrosyl-DNA phosphodiesterase 1 in genome stability is studied using a Medicago truncatula MtTdp1-depleted line. Lack of MtTdp1 results in a 39% reduction of methylated cytosines as compared to control. RNA-Seq analyses revealed that 11 DNA transposons and 22 retrotransposons were differentially expressed in the Tdp1-2a line. Among them all, DNA transposons (MuDR, hAT, DNA3-11_Mad) and seven retrotransposons (LTR (Long Terminal Repeat)/Gipsy, LTR/Copia, LTR and NonLTR/L1) were down-regulated, while the 15 retrotransposons were up-regulated. Results suggest that the occurrence of stress-responsive cis-elements as well as changes in the methylation pattern at the LTR promoters might be responsible for the enhanced retrotransposon transcription.