Subjects: Biology >> Botany >> Applied botany submitted time 2024-06-20 Cooperative journals: 《广西植物》
Abstract： In order to identify the pathogen that caused rust in Bletilla striata in Guangxi Zhuang Autonomous Region and screen resistene resources for B. striata, the rust pathogen was isolated from infected leaves of B. striata and identified using morphology and molecular methods. At the inoculation of pathogen. The results were as follows: (1) The urediniopores of Strain X2 isolated from diseased leaves in B. striata were golden yellow, oval, (21.43-30.95) μm × (13.10-19.05) μm. The teliospores were orange, russet, obovate or clavate, (17.25-30) μm × (5.5-6.65) μm. (2) The length of ITS sequence of strain X2(OQ826009) was 689 bp, which were compared with other ITS sequences in the GenBank, and the similarity reached to 95.86% compared with Coleosporium sp. (KY783686.1). But the sequence was clustered together with two sequences of C. bletiae (MN108161.1, OP363678.1). The strain X2 was identified as C. bletiae by combining morphology and molecular methods. (3) After 14 days of artificial inoculation with strain X2, the disease index of 23 Bletilla striata ranged from 0 to 70.7. And 6 different resistance levels were divided according to the disease index. Among them, one immune materials was identified for the disease index was 0. Four highly resistant materials was identified for the disease index was 1.7- 4.7. Six disease-resistant materials was identified for the disease index was 5.6-9.4. Five mediumresistant materials was identified for the disease index was 12.7-18.3. Five susceptible materials was identified for the disease index was 32.0-49.1 and two highly susceptible materials was identified for the disease index was 62.2-70.7. Five B. striata from diffenrent places can be promoted directely or applied as parent materials for creating excellent germplasm to resistant the rust in B. striata for they expressed immune or highly resistance. The five species came from Honghe City in Yunnan Province, Gongcheng County and Baise City in Guangxi Zhuang Autonomous Region, Zunyi City in Guizhou Province and Yichang City in Hubei Province, respectively. This study can provide a basis for further research on pathogenesis and control of rust in B. striata.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-09-20 Cooperative journals: 《广西植物》
Abstract： In order to identify the pathogens that caused tuber rot in Bletilla striata and study the inhibiting effects of herbal extracts on pathogens, the pathogens that caused tuber rot of Bletilla striata were isolated using usual tissue isolation. Morphological and molecular biological techniques were used to identify the strains. And seven herbal extracts were used to study the inhibiting effects on the pathogen. The results were as follows:（1） A total of 14 fungi and 4 bacteria were isolated from diseased leaves, leaf sheaths and tubers. But only strain GF-1 caused disease, whose symptoms consistent with those in the field. The incidences of GF-1 disease reinoculated in the field and laboratory were 100％, respectively. （2）GF-1 was identified as a memmber of Epicoccum, and its colonial morphology is a circular form, with white mycelium, prostrate on the medium, aerial, diaphragms and branches. There are conidia and chlamydospores. （3）At last, the sequence of internal transcribed spacer (ITS) region of GF-1 were analyzed, the length was 522 bp. The sequence was compared with other species in the GenBank and reached 99.62% similarity to E. sorghinum (MN493119.1) isolated form Sorghum, which was closer than others, including E. sorghinum (MF948994.1) isolated form leaves of B. striata. （4）GF-1 could be fully inhibited when the medium contained 0.1- 0.2 g·mL-1 extracts that extracted from 7 Chinese herbal, respectively. And it also could be fully inhibited by 0.05 g·mL-1 of C. cassia or Syringa oblate. In summary, the pathogen that caused tuber rot in Bletilla striata was identified as E. sorghinum. And GF-1 could be fully inhibited cultivated on the medium which contained 0.1- 0.2 g·mL-1 herbal extracts, e.g.: C. cassia, S. oblate, Cyclocarya paliurus, B. striata, Houpoea officinalis, Illicium verum or Cnidium monnieri.
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