分类: 物理学 >> 核物理学 分类: 天文学 >> 天文仪器与技术 提交时间: 2023-08-29
摘要: In the future, the Very Large Area gamma-ray Space Telescope (VLAST) is expected to observe high-energy electrons and gamma rays in the MeV to TeV range with unprecedented acceptance. As part of the detector suite, a high-energy imaging calorimeter (HEIC) is currently being developed as a homogeneous calorimeter that utilizes long bismuth germanate (BGO) scintillation crystals as both absorbers and detectors. To accurately measure the energy deposition in the BGO bar of HEIC, a high-dynamic-range readout method using a silicon photoMultiplier (SiPM) and multiphotodiode (PD) with different active areas has been proposed. A prototype readout system that adopts multichannel charge measurement ASICs was also developed to read out the combined system of SiPMs and PDs. Preliminary tests confirmed the feasibility of the readout scheme, which is expected to have a dynamic range close to 106.
分类: 生物学 >> 生物物理学 >> 生物物理、生物化学与分子生物学 提交时间: 2016-05-12
摘要: UNC-13-Munc13s have a central function in synaptic-vesicle priming through their MUN domains. However, it is unclear whether this function arises from the ability of the MUN domain to mediate the transition from the Munc18-1-closed syntaxin-1 complex to the SNARE complex in vitro. The crystal structure of the rat Munc13-1 MUN domain now reveals an elongated, arch-shaped architecture formed by a-helical bundles, with a highly conserved hydrophobic pocket in the middle. Mutation of two residues (NF) in this pocket abolishes the stimulation caused by the Munc13-1 MUN domain on SNARE-complex assembly and on SNARE-dependent proteoliposome fusion in vitro. Moreover, the same mutation in UNC-13 abrogates synaptic-vesicle priming in Caenorhabditis elegans neuromuscular junctions. These results support the notion that orchestration of syntaxin-1 opening and SNARE-complex assembly underlies the central role of UNC-13-Munc13s in synaptic-vesicle priming.