Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Animal Husbandry and Veterinary Medicine submitted time 2023-06-11
Sun, Honglei
Tong, Qi
Yu, Haili
Han, Qiqi
Li, Han
Chang, Haoyu
Qu, Yajin
Xu, Jie
Liu, Sidang
Liu, Jinhua
Abstract： The novel avian influenza viruses (AIVs) can cause serious economic losses to the poultry industry. Systematic surveillance of novel AIVs in poultry is essential for control of avian influenza. In the present research, we isolated H3N3 subtypes AIVs from chicken farms reporting illness in China during 2022–2023. All of these chickens showed respiratory disease signs and a 10-40% reduction in egg production. We conducted epidemiological surveys, virus isolation and identification, sequence analysis, and chicken infection experiments. The results showed that: 1) In December 2022, H3N3 AIVs was first found in a layer chicken flock in Eastern China, and the virus was transmitted to multiple Provinces with high density chicken populations in a short time. 2) Sequence analysis showed that the novel H3N3 AIVs were evolving as a triple reassortment event, which bears H3N8-derived HA gene, H10N3-derived NA gene and H9N2-derived internal genes. 3) The novel H3N3 AIVs were highly susceptible to SPF chickens. The virus replicated efficiently in the concha nasalis, trachea, lungs and Harders glands and infected chickens showed pathological damage. 4) H3N3 viruses were airborne transmission among chickens, whereas H3N8 viruses were not. In conclusion, the novel reassortment H3N3 virus showed pathogenicity and airborne transmissibility in chickens. Therefore, comprehensive surveillance of H3N3 AIVs in domestic poultry is imperative and control of the virus endemic is needed.
Peer Review Status:
Awaiting Review
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